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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14 results for: addgene/1000

Showing: 1 - 14 of 14 results

  1. Lentiviral Prep Service

    Type
    Collection
    ...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human... unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls. This backbone does not contain...

  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...proteins that result in a diverse set of colors. Addgene has assembled a collection of empty plasmid backbones...exctinction coefficient and quantum yield divided by 1000. pKa is the pH at which fluorescence intensity is...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource! Jump to Top...

  3. Antibody Guide

    Type
    Collection
    ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may...intensive. Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made...

  4. Bacterial Expression Systems

    Type
    Collection
    .... Additional Addgene Genome Engineering Resources Learn more about CRISPR with Addgene's CRISPR Guide ... Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain plasmids and an engineered...localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous...Pichia pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and purification... The cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is a vector library for the generation of...ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is a collection of Golden Gate compatible...activation or repression. The BIOFAB plasmid set (Addgene #1000000037) contains specific bacterial promoters that...

  5. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... distribution via Addgene. For a full list of Addgene's viral vectors See Addgene's Current AAV inventory...for distribution at Addgene: Penn ID Addgene ID Name Function PI AV-1-49531P 100040-AAV1 pAAV.hSyn1.Twitch2B.WPRE.SV40...Penn Vector Core and Addgene partnership to provide AAV viral preps, includes listing of transfer status...Pennsylvania Vector Core (Penn Vector Core) and Addgene previously partnered to provide high-quality AAV...be helpful to the scientific community. Penn ID Addgene ID Name PI AV-1-18916P 18916-AAV1 AAV-FLEX-rev-...36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P 100037-AAV1 ...Looger AV-1-ALL856 100048-AAV1 pAAV.CAG.LSL.tdTomato Hongkui Zeng AV-9-ALL856 100048-AAV9 pAAV.CAG.LSL....

  6. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...pick a library. The pooled CRISPR libraries in Addgene’s collection differ based on some important criteria...filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode Knockout...Blast 1000000173 Knockout Mouse Liu 3rd 10 185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid...Varies Bassik Mouse CRISPR Knockout Library 1000000121 — 1000000130 Knockout Mouse Bassik 3rd 10 Varies Berger...83978 1000000091 Activation Human Weissman 3rd 5 10 104,540 209,080 CRISPRa-v2 83996 1000000093 Activation...83969 1000000090 Inhibition Human Weissman 3rd 5 10 104,535 209,070 CRISPRi-v2 83987 1000000092 Inhibition...Birsoy 3rd 8 20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd...

  7. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: Oligomerized...article in which they were created, and include Addgene in the Materials and Methods of your future publications... Zinc Finger Consortium: OPEN Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists...

  8. Optogenetics AAV Preps

    Type
    Collection
    ...preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory Wild-type ChR2 ChR2...GFP Syn ChR2/H134R GFP Constitutive 8, rg* Boyden 100054 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 CAG ChR2...] CAG ChrimsonR tdTomato Flp dependent 8 Boyden 100049 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Syn...Syn ChETA EYFP Constitutive 1, 9 Deisseroth 100050 pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH CaMKII... EYFP Constitutive 1, 5, 9, PHPeB Dimidschstein 100061 pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry EF1a C1V1 (...

  9. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... transcription factor collection, available at Addgene. The collection can be used for screening transcription... entire pooled collection, can be purchased at Addgene. Each transcription factor isoform has a unique...ID PI Description MORF library 137000, 192821, 1000000218 Zhang A collection of human transcription factor...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ...plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids.... TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc ...amino acid repeats in the TAL effectors. View Addgene's TALEN Guide. These collections and accompanying...Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing ...Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  11. CRISPR Guide

    Type
    Collection
    ...window) . Addgene Website & Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101...cell lines Viral service at Addgene Viral plasmids and resources at Addgene Blog CRISPR topic page How ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...You Use a CRISPR Library? CRISPR libraries from Addgene are available in two formats: as liquid DNA, or...located. The majority of the CRISPR plasmids in Addgene’s collection are from S. pyogenes unless otherwise...Gene and Genome Editing , 3–4 , 100013. DOI: 10.1016/j.ggedit.2022.100013 Zetsche, B., Gootenberg, J. S....

  12. Biosensor AAV Preps

    Type
    Collection
    ...Ready-to-use AAV available from Addgene's viral service encoding biosensor tools, including calcium sensors...Cre dependent 5, 9 Tian Calcium Sensor: Twitch2B 100040 pAAV.hSyn1.Twitch2B.WPRE.SV40 Syn Twitch2B none...

  13. Adenovirus Plasmids

    Type
    Collection
    ...in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain modular...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection of neurodegeneration plasmids..._CTD_del321-343 TARDBP His T7 ALS Nicolas Fawzi 100092 pET28-his-3C_Tau352 MAPT His T7 Parkinson's, FTD...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 1 - 14 of 14 results