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We narrowed to 22 results for: cas9 expression vector

Showing: 1 - 20 of 22 results

  1. Zhang Lab CRISPR Page

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    .... The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another...constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with Cas9 in a wide...information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for cancer modeling... (20bp). CRISPR-Cas9 Cre expression vectors for genome editing in the brain Using Cas9 mice Platt et al...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system ...formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and...2013). SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human ...

  2. CRISPR Plasmids - Empty gRNA Vectors

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    ...the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR...all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase...cassettes are assembled into a Cas9 expressing vector. Yang A Robust CRISPR/Cas9 System for Convenient, High-Efficiency...single lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs...in which Cas9 has been integrated into the genome. gRNA Empty Vectors Many gRNA empty vectors have been... plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can cut, activate...meningitidis Joung Cas9 sgRNA vector 68463 Mammalian U6 none S. pyogenes Zhang Cas9/pTREX-n 68708 Other...

  3. Plant Plasmids and Resources

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    ...creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene silencing...intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series of vectors and DNA modules ...full selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. ...CRISPR/Cas9 Toolkit for use in a variety of plant species (e.g. maize and Arabidopsis) and vectors for easy... assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice...CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector... GreenGate Cloning System to create plant expression vectors containing several cassettes and generate...

  4. Plan Your Experiment

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    .../or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and ...Figure 1). You will decide how to express Cas9, the delivery system for Cas9 and the guide RNA (gRNA), the...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity...

  5. Lentiviral Prep Service

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    ... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to...4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral Service...

  6. Worm Expression Resources

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    ... genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific...Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous... the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans... via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...that expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens in a new window...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...and spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from...

  7. CRISPR References and Information

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    ...sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your...ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini and...CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF,... gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, ...gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro... Injection and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct ; pMA122...

  8. Zebrafish Plasmid Collection

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    ...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of...Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas...RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis...mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs...in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides ...

  9. CRISPR Plasmids - Tagging

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    ...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general... one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged...plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA, based on the Zhang...consists of two components: An all-in-one CRISPR/Cas9 vector based on the Zhang lab's PX330 , simultaneously...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the... that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for...

  10. CRISPR Plasmids - Prime Edit

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    ...contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection... top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...below. Use the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA (e.g...transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing...template. During prime editing, the pegRNA directs the Cas9 nickase to the target sequence where it nicks the...nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used in PE2...PE6 — small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding exonuclease...

  11. CRISPR Guide

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    ...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included in ...a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing...strand SpCas9-HF1 — disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading...more Cas9 fusion tools later in the guide. Figure 3: Overview of Cas9n. Increasing fidelity of Cas9 Researchers...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...

  12. AAV Molecular Tools

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    ...encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118...transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must...Cre-dependent expression of ER-localized TurboID (with HA and V5 tag). 8 Jonathan Long Overexpression Tools These...These AAV encode various genes for overexpression. ID Name Expression System Activity Serotype PI 58376...

  13. CRISPR Plasmids - dCas9-FokI

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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian ...Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian... CRISPR dCas9-FokI CRISPR: dCas9-FokI...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of the genome...

  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-...versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins ...Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP...tag; 2NLS; pCDNA3 vector backbone, mammalian expression pENTR4-myc-nuc - pEntry vector that adds a C-terminal...

  15. CRISPR Plasmids - Parasites

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    ...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Parasite"....CRISPR expression plasmids that have been designed for use in parasites. CRISPR...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...

  16. Botman-Teusink Yeast FP Collection

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    ...Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for...Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...

  17. Distribution to Industry

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    ...while also adding additional filters such as expression, vector type, species, and more. Plasmids Browse ...CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...

  18. Cre-Lox and Other Site-Specific Recombinases

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    ... plasmids expressing Cre recombinase and filter the results by expression system and vector type. Find... GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout/Knock-In... plasmids expressing Flp recombinase and filter the results by expression system and vector type. Find... plasmids expressing Dre recombinase and filter the results by expression system and vector type. Find...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with loxP...

  19. Tetracycline Inducible Expression

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    ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter ...Church 87360 TLCV2 Lentiviral vector for tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 ....Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...Experimental Considerations Expression System If your gene of interest should only be expressed occasionally, use...

  20. Bacterial Expression Systems

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    ... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...systems. ID Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline...
Showing: 1 - 20 of 22 results