We narrowed to 19 results for: cre recombinase
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TypeCollection...Plasmid Collections Recombinases Cre-Lox and Other Site-Specific Recombinases Cre Plasmids Dre Plasmids...your favorite site-specific recombinase (Cre, Flp, Dre, other recombinases). Use the search bars or sort...expressing Cre and other site-specific recombinases. Many of our in-stock viral preps feature Cre-dependent...Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome...Plasmids Flp Plasmids Other Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled...into two distinct families, serine recombinases and tyrosine recombinases, based on sequence similarity and...best characterized SSR systems, Cre and Flp, are tyrosine recombinases derived from P1 E. coli bacteriophage...
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Retrograde AAV viral preps
TypeCollection...AAV-pgk-Cre PGK Cre expression Recombinases Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate... 0.4.Cre.SV40 CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression...mCherry expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Deisseroth 55637...pEF1a-DIO-FLPo-WPRE-hGHpA EF1a Cre-dependent Flpo recombinase Recombinases Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent...expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases Wilson 105558... expression Recombinases Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases Wilson 55634...Flp-dependent Cre recombinase Recombinases Engel , Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression ... -
Deisseroth INTRSECT Collection
TypeCollection...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn Con/Foff EYFP Cre AND...AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT.../Von-EYFP Cre AND Flp AND Vcre Yes 137165 pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre No References...plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression of...approaches combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression of...intersectional cell population definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional... -
Zhang Lab CRISPR Page
TypeCollection...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...targeted to NeuN, plus Cre recombinase 60228 : sgRNA targeted to LacZ, plus Cre recombinase 60229 : sgRNA cloning...cloning backbone with Cre recombinase 60230 : sgRNA targeted to NeuN, plus Cre recombinase-2A-EGFP-KASH 60231...NeuN)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and an sgRNA...LacZ)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and an sgRNA...backbone)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and an sgRNA...cassettes, Cre recombinase-2A-EGFP-KASH and an sgRNA. #60231 - AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR... -
Penn Vector Core Partnership with Addgene
TypeCollection...pENN.AAV.hSyn.Cre.WPRE.hGH Cre Recombinase James M. Wilson AV-9-PV4175 105555-AAV9 pENN.AAV.hSyn.Cre.hGH Cre Recombinase James... 0.4.Cre.SV40 Cre Recombinase James M. Wilson AV-5-PV2408 105550-AAV5 pAAV.GFAP.Cre.WPRE.hGH Cre Recombinase...pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 Cre Recombinase James M. Wilson AV-9-PV2394 107788-AAV9 AAV.rTH.PI.Cre.SV40 Cre Recombinase... Recombinase James M. Wilson AV-10-PV1090 105537-AAV1 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James M...AAV.TBG.PI.Cre.rBG Cre Recombinase James M. Wilson AV-8-PV2004 105545-AAV8 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 Cre Recombinase... 0.4.Cre.SV40 Cre Recombinase James M. Wilson AV-1-PV2676 105553-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH Cre Recombinase...Wilson AV-1-PV1090 105537-AAV1 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James M. Wilson AV-1-PV1848 105540... -
Recombinases AAV Preps
TypeCollection...Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following... site-specific recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page...Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11, rg* Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9,...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1,....CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry...tdTomato 9 Pu 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D ... 5 Carmichael 107788 AAV.rTH.PI.Cre.SV40 rTH none 9, rg* Wilson 24593 AAV-pgk-Cre PGK none rg* Aebischer... -
Brain Initiative Collection
TypeCollection...83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAVrg pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-... -
Brain Armamentarium
TypeCollection...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression in striatal...pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase expression in striatal...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression in striatal...in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...network function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium Project... -
Caltech Systemic Capsids
TypeCollection...105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression... Fishell Recombinases 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550...Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV...Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent ...sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent...sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG Calcium sensor, Cre-dependent...Activator, Cre-dependent ChR2 Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG Activator, Cre-dependent... -
Kazuhiro Oka Lentiviral Vectors
TypeCollection...infectious virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP... a variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer...tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255... 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1-DIO-tdTomato...72255 NOT AVAILABLE YET Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express... pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato... -
Tetracycline Inducible Expression
TypeCollection...Neo-TRE-CMV-Cre-rtTA Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible...pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of Cre recombinase. Takuji Iwasato...pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase. Floris Foijer...-inducible expression of a light-activated Cre recombinase. Jacco van Rheenen 104102–104112 TetBow Series...Repressible Promoters Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids...tetracycline-controlled transactivator (tTA) was created by fusing TetR with the activation domain of the...tetracycline-controlled transactivator (rtTA) was created by fusing rTetR with VP16. In the absence of tetracycline... -
AAV Viral Preps
TypeCollection...Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate... -
Retrovirus Plasmids
TypeCollection...63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian ...overexpression plasmid; deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the ...largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized cells (see Weinberg Lab page for...pBABE-hygro-hTERT MoMLV Expression of hTERT for creation of immortalized cells; additional plasmids include...cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE... post-transcriptional regulatory element that increases export to the cytoplasm Hahn 11375 MDH1-PGK-GFP... -
Worm Expression Resources
TypeCollection...RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are.... elegans by combining expression of the FLP recombinase from either a heat-shock promoter or a tissue-specific... -
Bacterial Expression Systems
TypeCollection...Ashwani Kumar 134405 pBbAW4k-loxP-TT-loxP-mRFP1 Cre recombinase activity Fluorescence (mRFP1) Escherichia coli... Check our Cre-Lox Plasmids Collection for a selection of plasmids containing recombinases to induce targeted...Guide CRISPR Guide Bacteria are commonly used to create, store, and replicate plasmids of all types, but...and an engineered strain for a B2H system used to screen the activity of arrays of zinc finger nucleases...benzoate (cumate) Sphingomonas sp. , Caulobacter crescentus , Paracoccus denitrificans , Methylobacterium... -
CRISPR Guide
TypeCollection...Plasmids: CRISPR transposases CRISPR Recombinases and Integrases Recombinases, and their subfamily integrases...for large scale edits. These proteins, like Cre recombinase or phage derived serine integrases, insert ...mechanisms, including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon...lab , pairs prime editing with a large serine recombinase (LSR). A prime editor is used to install the ...evoBxb1 or eeBxb1 are evolved versions of the recombinase with improved integration efficiencies in mammalian...& Hsu, P. D. (2022). Systematic discovery of recombinases for efficient integration of large DNA sequences... GAT; increase nuclease fidelity SpCas9-NG - NG; increase in vitro activity SpG - NGN; increase nuclease... -
CRISPR Plasmids - Tagging
TypeCollection...vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid...selection markers are available. An improved FLP recombinase expression vector allows for efficient marker..._CEBPA_1 PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...genome modification. This system can be used to create C- and N-terminal epitope tags. The plasmids in...has developed a systematic and scalable method to create marker-free mutations, insertions, and deletions...stem cell (hiPSC) lines. These cell lines were created using CRISPR and the donor plasmids containing ... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...Isocortex 230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT Isocortex 214583 pAAV-AiE2543m-minBG-SYFP2...Isocortex 220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex 224004 pAAV-AiE0680m-minBG-SYFP2...Isocortex 230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT Isocortex 223974 pAAV-AiE0050m...224056 pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA AiP13152 AiE0050m_3xC2 Cre Layer 4_IT Isocortex 224038 ...220734 pAAV-AiE0671m_3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_IT Isocortex 224043 ...220735 pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_IT Isocortex 214522 ...229920 pAAV-AiE0459m_3xC2-minBG-iCre(R297T)-BGHpA AiP14187 AiE0459m_3xC2 Cre Layer 5_ET Isocortex 224013 ... -
CRISPR References and Information
TypeCollection... (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid summary...- a CRISPR Screen Tool (Link opens in a new window) Design gRNAs for custom CRISPR screens targeting a...in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable eBook about...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole ...batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) ...considerations, and more. CasPEDIA is a community resource created and maintained by the Jennifer Doudna Lab . Read...