We narrowed to 38 results for: gfp control plasmid
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TypeCollection...pAAV-CAG-GFP CAG GFP Control Boyden 50465 pAAV-hSyn-EGFP hSyn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKIIa... 135631 pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein 192552...pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI....CamKIIa EGFP Control Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell...pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10 These viral vector preparations...
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Brain Initiative Collection
TypeCollection...under the control of the mDlx enhancer element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression...under the control of the mDlx enhancer element Gordon Fishell 83900-AAV2 pAAV-mDlx-GFP-Fishell-1 GFP expression...under the control of the mDlx enhancer element Gordon Fishell 83900-AAV5 pAAV-mDlx-GFP-Fishell-1 GFP expression...under the control of the mDlx enhancer element Gordon Fishell 83900-AAV8 pAAV-mDlx-GFP-Fishell-1 GFP expression...under the control of the mDlx enhancer element Gordon Fishell 83900-AAV9 pAAV-mDlx-GFP-Fishell-1 GFP expression... the control of the mDlx enhancer element Gordon Fishell 83900-AAVrg pAAV-mDlx-GFP-Fishell-1 GFP expression... the control of the mDlx enhancer element Gordon Fishell 83900-PHPeB pAAV-mDlx-GFP-Fishell-1 GFP expression... -
Retrograde AAV viral preps
TypeCollection...Dlx NLS-mRuby2 Control Viviana Gradinaru 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Gordon Fishell ... 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent...105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control James M. Wilson 116869 pAAV-CAG-H2B-GFP CAG GFP Control...Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control Gordon Fishell 83894 pAAV-hDlx-Flex-dTomato-Fishell...Cre-dependent Control Hongkui Zeng 50465 pAAV-hSyn-EGFP Syn EGFP Control Bryan Roth 50469 pAAV-CaMKIIa-EGFP CamKII...CAG tdTomato Control Edward Boyden 50457 pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent Control Bryan Roth ...CamKII EGFP Control Bryan Roth 114472 pAAV-hSyn-mCherry Syn mCherry Control Karl Deisseroth 114469 pAAV-CaMKIIa-mCherry... -
Penn Vector Core Partnership with Addgene
TypeCollection...pAAV.GfaABC1D.PI.Lck-GFP.SV40 Control Baljit Khakh AV-5-PV2380 100896-AAV5 pAAV.GFA104.PI.eGFP.WPRE.bGH Control Philip...pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-9-PV0101 105530-AAV9 pAAV.CMV.PI.EGFP.WPRE.bGH Control James ...pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng....SV40 Control Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren ...pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-2-ALL854 51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng...pAAV.CMV.LacZ.bGH Control James M. Wilson AV-5-PV1917 105541-AAV5 pENN.AAV.CamKII0.4.eGFP.WPRE.rBG Control James ...pENN.AAV.CMVs.TurboRFP.WPRE.RBG Control James M. Wilson AV-5-PV2213 105547-AAV5 pENN.AAV.EF1a.eGFP.WPRE.rBG Control James M. ... -
Control AAV Preps
TypeCollection...CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Viviana Gradinaru 116869 pAAV-CAG-H2B-GFP CAG H2B-GFP Constitutive...Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our control AAV that express...quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including fluorescent...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 6, 8, 9, 11, rg*, PHPeB...PHP.eB, PHP.S Edward Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB ...Constitutive 1 Kiryl Piatkevich 214147 pAAV-hIBA1a-GFP-miR124T hIBA1a GFP Constitutive 5 Chun-Li Zhang 20299 pAAV-EF1a-double... Viviana Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Gordon Fishell... -
Viral Production
TypeCollection...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview of... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma The 293T... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...Addgene's viral production methods and quality control for lentivirus and AAV. Viral...virus preps are titered and subjected to quality control by Addgene before being distributed to customers... -
Lentiviral Prep Service
TypeCollection...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource...Purpose PI 17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV...Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our... 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 ...library expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse...our repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from the...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert... -
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid CLIP-tag...pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid...SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag Control for cell...CLIP-tag Control for mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-tag Control for cell...pMCP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101131 pACP-GPI Control Plasmid...-tag Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag Control for cell...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental... -
Serotype Testing AAV
TypeCollection...). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various...These AAV are made from plasmid 37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG... AAV available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing...37825-AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various...These AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human...list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized... catalog item, including the titer and quality control, visit the material page (linked for each catalog... -
Tetracycline Inducible Expression
TypeCollection...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters...Lentiviral plasmid for Tet-controlled expression of transgene of interest with EGFP (On or Off) None TRE, miniCMV...the transactivator and the tet-controlled gene of interest from one plasmid. A single vector approach may... to use for cloning to control expression of your gene of interest. ID Plasmid Description Transactivator...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for ...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible... -
Bacterial Expression Systems
TypeCollection...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also...Expansion Collection Microbiology Plasmids Collection Synthetic Biology Plasmids Collection Empty Backbones ...under the microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent...New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and... This collection contains plasmids whose expression levels can be controlled by a variety of small molecules... -
Optogenetics AAV Preps
TypeCollection...pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Jaws GFP Constitutive 5, 8, rg* Edward Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2...neuroscience to control electrical potentials in neurons. See our Optogenetics Plasmids and Optogenetics...Karl Deisseroth 58880 pAAV-Syn-ChR2(H134R)-GFP Syn ChR2/H134R GFP Constitutive 8, rg* Edward Boyden 100054...Gradinaru 107708 pAAV-hSynapsin- soCoChR-GFP Syn CoChR (soma-targeted) GFP Constitutive 9 Edward Boyden 107712...107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn CoChR (soma-targeted) GFP Cre dependent 9 Edward Boyden 35505 ...Deisseroth 122063 pAAV-EF1α1.1-ChrimsonR-GFP EF1a ChrimsonR GFP Constitutive 2 Edward Boyden 124650 pAAV-CamKIIa-C1V1...Christopher Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, rg* Edward Boyden 59171... -
Luciferase Plasmid Collection
TypeCollection...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...interest in a controlled environment under various conditions. When choosing what plasmid backbone to use...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral... -
Antibody Production
TypeCollection... undergo stringent quality control by Addgene or a trusted quality control partner before distribution... to an untransfected control and the protein size is confirmed. Residual Plasmid Sanger Sequencing Purified... low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...Addgene's antibody production methods and quality control processes for purified recombinant antibodies. ...production protocols, titering methods, and quality control are described below. Information for a specific...embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light chains. Antibodies...samples with >90% purity are distributed. Quality Control Each lot of antibody undergoes testing, either ... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection... with or without GFP and mCherry controls. Do you have suggestions for other plasmids that should be added... Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...out PCR for 10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled... 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library... added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve... -
Zhang Lab CRISPR Page
TypeCollection...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA...sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: These plasmids contain two expression...guide acceptor: The PX552 plasmid (#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor...acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...present within the mouse genome. This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA... -
Bikard Lab - CRISPR Repression Collection
TypeCollection... reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding...collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds to sfgfp with either 0, ...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols...target sequence lower the repression level. By controlling the number of mismatches we can obtain a range...18, or 20 matching nucleotides. Together, these plasmids allow to explore the whole range of expression...amount and stoichiometry of enzymes. The pLC97 plasmid allows for quick integration of the aTc-inducible... -
AAV Molecular Tools
TypeCollection...-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev...lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev... These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used...Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals...5, 8 Nirao Shah , Jim Wells 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent expression...expression of diphtheria toxin receptor fused to GFP for studying cell ablation. 2 Thomas Jessell , Eiman Azim...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need.... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations, MERS and...David Nemazee 161030 Zhou-pLOV-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter...