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We narrowed to 13 results for: ins/1000

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Showing: 1 - 13 of 13 results

  1. Lentiviral Prep Service

    Type
    Collection
    ...unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human... unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,674 mouse genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...

  2. Antibody Guide

    Type
    Collection
    ...sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may...four protein chains: two large heavy chains and two small light chains. These four domains come together...their monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin ...ankyrin repeat proteins. These are antibody mimics instead of modified antibodies and are not closely related...synthetic binding proteins which use the Fibronectin type III domain as their scaffolding instead of the antibody...Denature proteins, using heat and/or chemicals, and run on an SDS-PAGE gel to separate proteins by size...your antibody’s specificity. A blank (contains water or buffer instead of a biological sample), which captures...

  3. Plant Plasmids and Resources

    Type
    Collection
    ...Description Cloning Type PI 1000000147, 1000000148, 1000000149, 1000000150 2in1 Plasmid Toolkit Plasmids...Lenaghan 1000000241 GreenGate 2.0 Toolkit Compatible with GreenGate Cloning System (#1000000036). Includes... Voytas 1000000241 GreenGate 2.0 Toolkit Compatible with GreenGate Cloning System (#1000000036). Includes... Gene Silencing Reporters Addgene’s repository contains many plasmid tools that were specifically designed...expression vectors. ID Kit Description Cloning Type PI 1000000036 GreenGate Cloning System Plasmids for rapidly...Gate method. Golden Gate (GreenGate) Jan Lohmann 1000000076 GoldenBraid 2.0 Kit Plant synthetic biology kit...parts. Golden Gate (GoldenBraid) Diego Orzaez 1000000135 MoClo Plant Parts II and Infrastructure Kit Modules...

  4. Bacterial Expression Systems

    Type
    Collection
    ...biofactories to express plasmids and produce proteins in high yields. Proteins can then be extracted from the culture...recombinant proteins to the periplasmic space between the inner and outer membranes. Targeting proteins to the...Plasmid Collection contains plasmids for non-toxic protein labeling and visualization inside living or fixed...Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent ...In BiFC, proteins of interest are fused to each part of a split FP. When the candidate proteins interact...Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain plasmids and an engineered strain...repression. The BIOFAB plasmid set (Addgene #1000000037) contains specific bacterial promoters that vary in...

  5. Optogenetics AAV Preps

    Type
    Collection
    ...optogenetics AAV collection, including channelrhodopsins, halorhodopsins, and more....Inhibitory Arch and variants eNpHR and variants InSynC (non-opsin) Jaws WiChR stGtACR iC++ OPN3 PdCO PPO...ChR2/H134R GFP Constitutive 8, rg* Edward Boyden 100054 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 CAG ChR2...ChrimsonR tdTomato Flp dependent 8 Edward Boyden 100049 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Syn...Syn ChETA EYFP Constitutive 1, 9 Karl Deisseroth 100050 pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH CaMKII...Constitutive 1, 5, 9, PHPeB Jordane Dimidschstein 100061 pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry EF1a C1V1 (...Edward Boyden 50972 AAV-SYP1-miniSOG-T2A-mCherry Syn InSynC (non-opsin) mCherry Constitutive 8 Roger Tsien ...

  6. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ... (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to ... packaged plasmids and 3 individually packaged strains, which are shipped as bacterial stabs at room temperature...publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: Oligomerized pool...

  7. Control AAV Preps

    Type
    Collection
    ... control AAV collection, including fluorescent proteins, spaghetti monster, and more....Browse our control AAV that express fluorescent proteins under general promoters. These AAV can be used...Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Viviana Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet Constitutive...dTomato Cre dependent 1, 2, 5, 9, rg* Gordon Fishell 100043 pAAV.synP.DIO.EGFP.WPRE.hGH Syn EGFP Cre dependent...

  8. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...comprehensive human transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two ...scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is a comprehensive...ID PI Description MORF library 137000, 192821, 1000000218 Zhang A collection of human transcription factor...

  9. Adenovirus Plasmids

    Type
    Collection
    ...with large inserts Vogelstein 1000000176 AdenoBuilder Toolkit Plasmids containing modular inserts that span...reaction Bunz Return to top Adenoviral Strains Browse bacterial strains for adenoviral vector production. ...plasmids, adenoviral backbone plasmids, and bacterial strains for adenoviral vector production in Addgene's repository...Shuttle Plasmids Adenoviral Plasmids Adenoviral Strains Adenoviral vectors are widely used for gene delivery... Search our database for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production...16407 pAdEasy 2-GFP beta-gal Test plasmid that contains β-gal and GFP and a ∼10 Kb genomic DNA stuffer...Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains the pAdEasy-1 (Addgene #16400) plasmid Vogelstein...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ... System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one ...plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru...message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden Gate TALEN 2.0 36033...sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this...under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression...mutation induction when using TALENs. The FokI domains (DD, RR) used are obligate heterodimers that require...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...

  11. CRISPR Guide

    Type
    Collection
    ...inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ...exhibit almost unidirectional insertions but are more prone to off-target insertions. And in the case of both... edits. These proteins, like Cre recombinase or phage derived serine integrases, insert donor DNA at “...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...

  12. Biosensor AAV Preps

    Type
    Collection
    ...Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under the control ...minBG RiboL1-jGCaMP8s none Constitutive 1 Allen Institute for Brain Science , Jonathan Ting Calcium Sensor...dependent 5, 9 Lin Tian Calcium Sensor: Twitch2B 100040 pAAV.hSyn1.Twitch2B.WPRE.SV40 Syn Twitch2B none...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV Parkinson's L. Miguel...Miguel Martins 14124 pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2...Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag CMV Parkinson's L. Miguel Martins 15940 pcDNA3-HtrA2...Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's L. Miguel Martins 16155 pET20b-...His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's L. Miguel Martins 16158 pET20b-...
Showing: 1 - 13 of 13 results