We narrowed to 17 results for: lenti crispr plasmid
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TypeCollection... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...Packaging Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes...libraries 92385 Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A Human CRISPR inhibition library...Root 92386 Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set B Human CRISPR inhibition library...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,...GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids...
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Lentivirus Plasmids
TypeCollection...Looking for lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer ... Viral Plasmids Lentiviral Plasmids Lentiviral...of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway ... Also see plasmid 46948 . Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection... CRISPR Empty gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited... limited to a single CRISPR function. Alternatively, gRNA-only plasmids can be used in cells or organisms.... Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that...none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none, need plasmid 65776 S. aureus... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome...-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our Empty...of your cells will take up your plasmid. Thus, many plasmids have markers on them so that you... -
Luciferase Plasmid Collection
TypeCollection...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing plasmid and the effects...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout plasmid. Renilla luciferase is used to monitor ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...luciferase Scott Lowe 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase... -
CRISPR Guide
TypeCollection...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...miRNAs to restrict CRISPR editing only to the target tissue. Browse Plasmids: Anti-CRISPR Small Precision...modification using CRISPR Browse Plasmids: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging...genomic regions using CRISPR Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S.... -
CRISPR References and Information
TypeCollection...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library...Description Plasmids in protocol Download protocol Addgene CRISPR pooled library amplification CRISPR pooled... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community... -
Zhang Lab CRISPR Page
TypeCollection... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...invading phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas...PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery... -
Plan Your Experiment
TypeCollection... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene depositors )...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...relevant. Delivery Using Plasmids The most straightforward method to deliver a CRISPR system into mammalian... -
CRISPR Pooled gRNA Libraries
TypeCollection... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function Genome...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ...virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...steps for using pooled CRISPR libraries and keep the following in mind: CRISPR screening experiments require...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ... -
CRISPR Plasmids and Resources
TypeCollection...preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used ...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed.... C. elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for ... Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for... parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR... -
Plasmid Collections
TypeCollection...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 161,184 plasmids . We've...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent proteins...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids from a depositor organized...Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection for Solute Carrier Proteins... -
Plasmids for Stem Cell Research
TypeCollection... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent...RNA p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive...Differentiation and Transdifferentiation Plasmids Browse the table below for plasmids used for differentiation of ...Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms ... -
Neurodegeneration Research Collection
TypeCollection... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ... -
Neurodegeneration Plasmid Collection
TypeCollection...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...Bonifacino 173579 Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...others. The table below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent... -
Tetracycline Inducible Expression
TypeCollection...Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...system. ID Plasmid Description Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On ...KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma Rawlins 72835 pMK243 (Tet-OsTIR1-PURO) Expresses... -
Immunology Research Plasmids and Resources
TypeCollection...Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...rapidly initiate a secondary immune response. Browse Plasmids for Immunology Research Genes have been grouped...