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We narrowed to 27 results for: lentiviral plasmid

Showing: 1 - 20 of 27 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent... Myc, and Brd3R from five separate lentiviral plasmids The acetyllysine reader BRD3R promotes human nuclear..., and Lin28 from four separate lentiviral plasmids Induced Pluripotent Stem Cell Lines Derived from Human...Sox2, Klf4, and c-Myc from four separate lentiviral plasmids A drug-inducible system for direct reprogramming...

  2. Lentivirus Plasmids

    Type
    Collection
    ...Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer ... Viral Plasmids Lentiviral Plasmids Lentiviral...of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...Looking for lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table... Also see plasmid 46948 . Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based...

  3. CRISPR Plasmids - Empty gRNA Vectors

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    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited.... Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome...

  5. Luciferase Plasmid Collection

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    Collection
    ...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... Plasmid Collections Luciferase...Luminescent Imaging with Nano-lanterns Promega Plasmid Collection Luciferase is the enzyme responsible...

  6. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...

  7. Kazuhiro Oka Lentiviral Vectors

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    ... Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors...Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral and ...Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and Resources Addgene Blog...deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors ...Vectors Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not...infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral...

  8. Viral Production

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    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...our virus protocols . Browse our plasmid collection of popular lentiviral vectors ....AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR...additional plasmids. tetR-KRAB TRE Patrick Aebischer , Didier Trono 41393 pCW57.1 Lentiviral Tet-On vector...Tight TRE Markus Ralser 64238 pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression of transgene...

  10. Mammalian RNAi Tools

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    Collection
    ...viral delivery of a plasmid. shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression...protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid... Plasmid Collections Mammalian RNAi Mammalian shRNA...

  11. Lentiviral Prep Service

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    Collection
    ... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our repository...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository ...available in lentiviral format. Viral...repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from the inventory...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits,...

  12. Plan Your Experiment

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    Collection
    ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular option for producing...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene ...

  13. Zhang Lab CRISPR Page

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    Collection
    ... Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice...

  14. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Species) Organism/Expression System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP...Verkhusha Lab Plasmids - Enhanced colors, including PSmOrange, iRFP Gradia Lab Mammalian Plasmids - Includes...Lab Plasmids - Includes many N- and C-terminal fluorescent proteins Insect Sutherland Lab Plasmids - For...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...colors. Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you...

  15. Viral Prep Service for Plasmids and Pooled Libraries

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    Collection
    ...the right to use the original plasmid. Distribution of the original plasmid is important because it is the... service for plasmids and pooled libraries. Viral preparation options include lentivirus and AAV. Viral...produces high-quality viral vector preps from select plasmids in our collection. We know that viral vector production... production is a common step after requesting plasmids from Addgene, and by providing this service, we...In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer preps...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene...

  16. CRISPR Plasmids and Resources

    Type
    Collection
    ... of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety .... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in...

  17. Adenovirus Plasmids

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    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

  18. Retrovirus Plasmids

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    Collection
    ...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

  19. CRISPR Guide

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    Collection
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...

  20. Neurodegeneration Research Collection

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    Collection
    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...
Showing: 1 - 20 of 27 results