...expression of dLight1.1 under synapsin promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated...for expression of dLight1.1 under CAG promoter Lin Tian 111067-AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated...for expression of dLight1.1 under CAG promoter Lin Tian 111068-AAV1 pAAV-hSyn-dLight1.2 To generate Adeno-Associated...expression of dLight1.2 under synapsin promoter Lin Tian 111068-AAV5 pAAV-hSyn-dLight1.2 To generate Adeno-Associated...expression of dLight1.2 under synapsin promoter Lin Tian 111069-AAV1 pAAV-CAG-dLight1.2 To generate Adeno-Associated...for expression of dLight1.1 under CAG promoter Lin Tian 111261-AAV9 pAAV-hSynapsin1-axon-GCaMP6m For enriched... enriched expression of GCaMP6m in axons Lin Tian 111262-AAV1 pAAV-hSynapsin1-axon-GCaMP6s For enriched...

...Constitutive 1 Tian 112000 pAAV-hSynapsin1-axon-GCaMP6f Syn axon-GCaMP6f none Constitutive 1 Tian 112005 pAAV-hSynapsin1...5, 9 Tian 112010 pAAV-hSynapsin1-FLEx-axon-GCaMP6s Syn axon-GCaMP6s none Cre dependent 5, 9 Tian Calcium...Constitutive 1 Tian 111067 pAAV-CAG-dLight1.1 CAG dLight1.1 none Constitutive 1, 5 Tian 111068 pAAV-hSyn-dLight1.2...Constitutive 1, 5 Tian 125560 AAV-CAG-dLight1.3b CAG dLight1.3b none Constitutive 1, 5, 9 Tian 135762 pAAV-...none Constitutive 9 Tian 125707 pAAV-CAG-RdLight1 CAG RdLight1 none Constitutive 9 Tian 125708 pAAV-hSynapsin1...Constitutive 9 Tian 128486 pAAV-CAG-FLEx-iSeroSnFR CAG iSeroSnFR none Cre dependent 9 Tian 163909 pAAV_hSynapsin_psychLight2...axon-GCaMP6m Syn axon-GCaMP6m none Constitutive 9 Tian 131004 pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE...

.... 2018 Jun 29;360(6396). pii: science.aat4422. Lin Tian Dopamine Yellow or Red genetically encoded dopamine... vivo. Nat Methods. 2020 Nov;17(11):1147-1155. Lin Tian Endocannabinoid GRAB_eCB2.0/eCBmut biosensor for...Cell. 2020 Dec 12. pii: S0092-8674(20)31612-3. Lin Tian Serotonin Serotonin (5-HT) sensor psychLight Psychedelic-inspired... Cell. 2021 Apr 8. pii: S0092-8674(21)00374-3. Lin Tian Serotonin Cilia-targeted serotonin sensor GRAB-HTR6...In Vivo. Cell. 2016 Jun 30;166(1):245-57. Michael Lin Voltage Ultrafast fluorescent voltage sensor for ...voltage sensor. Nat Neurosci. 2014 Apr 22. Michael Lin Voltage Fluorescent voltage reporters Archon 1 and...high-light signalling mechanism. Nat Commun. 2017 Jun 29;8(1):49. Philip Mullineaux Insulin Sensor for...

...1171. PMID: 23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X.,...Science , 339 (6121), 819–823. PMID: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang,...L., Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., & Liu, D. R. (2018). Evolved Cas9 variants with..., Richter, M. F., Everette, K. A., Banskota, S., Tian, K., Tao, Y. A., Tolar, J., Osborn, M. J., & Liu...Molecular Sciencess . 25 (5), 2456. PMID: 38473704 Lin, S., Staahl, B. T., Alla, R. K., & Doudna, J. A. ..., S., Pruitt, B. W., Tuttle, M., Iyer, E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand, D. J., Ter-Ovanesyan...single-stranded DNA, unraveling DNA as it goes using its helicase properties, often called a reeling mechanism. ...

...PubMed (Link opens in a new window) Gao ZR, Chen WZ, Liu MZ, Chen XJ, Wan L, Zhang XY, Yuan L, Lin JK, Wang...recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing animal lines and retro-grade...producing their own INTRSECT plasmids. Online spreadsheet (Link opens in a new window) of Flp-recombinase-expressing...transgenic mouse and rat lines. The Fenno Lab maintains a public folder (Link opens in a new window) of...lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786. PubMed (Link opens in a new window...International 129:104482. PubMed (Link opens in a new window) Poulin JF, Caronia G, Hofer C, Cui Q, Helm... INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular...

...-COMP-b-lac His-tag Cd4d3+4-6his Bio-linker-his Cd4d3-bio-linker-6His* FLAG prey CD200-Cd4d3+4-COMP-blac...Mouse sperm Mouse egg Laboratories using AVEXIS: Christian Sollner, Max Planck Institute Mitch Levesque University...and its resolution by spatiotemporal expression profiling. Martin S, Söllner C, Charoensawan V, Adryan B...

...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...examples of useful worm CRISPR resources, along with links to protocols, can be found below: SapTrap-SEC kit...Jorgensen Lab. A collection of plasmids that streamlines the production of targeting vectors for tag insertion...from Michael Nonet. Protocol can be found at the (Link opens in a new window) Seydoux lab website . Co-...on miniMos and MosSCI plasmids can be found on Christian Frøkjær-Jensen's Wormbuilder website. General ..., which diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression...

...Bogdanove TALEN kit came online in the summer of 2011 and has already become the best-selling kit in Addgene’s... effectors, scientists in several biological disciplines are coming closer to this DNA targeting ideal...arrays from 12-31 repeats. Perhaps one of the most appealing features of the TAL effector arrays is the ease...can be made. Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into...early in 2011 and they described their kit for assembling TAL effector arrays in a 2012 issue of Nature...Whether you work in a C. elegans lab and have been struggling to mutate a specific gene or your anxiously investigating...constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia...