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We narrowed to 16 results for: mcherry reporter

Showing: 1 - 16 of 16 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells pBI-MCS-EGFP -...GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression pLV-mCherry - 3rd...tagging vectors in budding yeast pHT101-mCherry - Expresses mCherry tagged fusion proteins in worms ins:MCS...MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable marker...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent...

  2. Bacterial Expression Systems

    Type
    Collection
    ...Christopher Voigt 65008 pCRISPReporter-mCherry Promoter activity Fluorescence (mCherry) Escherichia coli Mattheos...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...Tolker-Nielsen 90217 pMV762-Peredox-mCherry NADH:NAD+ ratio Fluorescence (mCherry) Mycobacterium sp. Ashwani Kumar...Fluorescence (mCherry) Escherichia coli David Summers 24659 pCHERRY3 Promoter activity Fluorescence (mCherry) Mycobacterium...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...

  3. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry to genes of interest, ...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...or 20 matching nucleotides, the second binds to mcherry with either 0, 11, 18, or 20 matching nucleotides...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...

  4. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection. Dapeng...

  5. Chemogenetics AAV Preps

    Type
    Collection
    ...and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry hM3D(Gq) - Activation...pAAV-hSyn-DIO-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth...Roth 50458 pAAV-hSyn-DIO-rM3D(Gs)-mCherry rM3D(Gs) - Activation mCherry fusion Cre-dependent 8 Bryan Roth...Roth 50474 pAAV-hSyn-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan...Bryan Roth 50475 pAAV-hSyn-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 2, 5, 8, 9, rg* Bryan...154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent 8, rg* ...154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent 8, rg* ...

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...

  7. Viral Production

    Type
    Collection
    ...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  8. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes...

  9. Fluorescent Proteins: FRET

    Type
    Collection
    ...mCitrine-pBAD EGFP mCherry 488 0.60 610 72,000 0.22 5.3 1.9 mEGFP-N1 , mCherry2-N1 , pcDNA3.1 CMV mCherry-eGFP BGH..., mKO2-N1 mOrange2 mCherry 549 0.60 610 72,000 0.22 6.1 4.4 mOrange2-N1 , mCherry2-N1 miRFP670 miRFP720...single-nanometer scale, scientists can use FRET to report on protein binding or conformational changes. Organic...your experiment, be sure to refer to published reports and include appropriate controls to help interpret...

  10. Lentivirus Plasmids

    Type
    Collection
    ...resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven expression of mCherry fusion. See plasmids 36083 and...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452...

  11. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...second expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry fragment. Upon...Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid 112030 pNAS1b split mCherry NEDD4 WW2 neg ctrl Plasmid...112029 pNAS1b split mCherry NEDD4 WW2 pos ctrl Plasmid 111883 pNAS1b split mCherry NEDD4-2 WW2 Plasmid...Plasmid 111882 pNAS1b split mCherry NEDD4 WW2 Plasmid 111881 pNAS1b split mCherry 14-3-3σ Plasmid 111880 pNAS1b...the phosphobinding domain and the phosphosite, mCherry fluorescence is restored, so interactions can be...pNAS1b split mCherry 14-3-3β Pooled Library 111708 Mode #2 Library (NEDD4-2 WW2 domain) Pooled Library...

  12. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 83898-AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...Gordon Fishell 83898-AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic... Fishell 83898-AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...135634-AAV1 pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder...135634-AAV9 pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder...-PHPeB pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder...expression. Bryan Roth 75033-AAV1 pAAV CD68-hM4D(Gi)-mCherry Expression of hM4D(Gi) using the CD68 promoter....

  13. Caltech Systemic Capsids

    Type
    Collection
    ...pAAV-CaMKIIa-mCherry CamKIIa mCherry Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth...pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor...tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (...Activator C1V1 Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator ChR2 Dimidschstein...213815 pAAV_BiSSTe10_ChR2_mCherry BiSSTe10 Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27...213856 pAAV_BiVIPe4_ChR2_mCherry BiVIPe4 Activator ChR2 Fishell 213915 pAAV_BiLAMP5e3_ChR2_mCherry BiLAMP5e3... 213937 pAAV_BiPVe4_ChR2_mCherry BiPVe4 Activator ChR2 Fishell 213941 pAAV_BiPVe3_ChR2_mCherry BiPVe3 ...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...Calcium Ratiometric calcium biosensors from nested reporters of green- and orange-emitting proteins Ratiometric...imaging (Perceval) A genetically encoded fluorescent reporter of ATP:ADP ratio. Nat Methods. 2009 Feb . 6(2)...

  15. Genetic Code Expansion

    Type
    Collection
    ... express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells. You ...Abhishek Chatterjee 220800 EF1a-L274GMmMetRS-T2A-mCherry MetRS M. musculus azidonorleucine (Anl) Mammalian...RF1 function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all...removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG...

  16. Tetracycline Inducible Expression

    Type
    Collection
    ...efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet, miniCMV Howard Gu...monitored by the dilution of a dox-inducible H2B-mCherry marker. Aviv Regev , Joan Brugge Content last reviewed...
Showing: 1 - 16 of 16 results