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We narrowed to 12 results for: Cre expression vector

Showing: 1 - 12 of 12 results

  1. Adenovirus Guide

    Type
    Guide
    ...Guides Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production Integration...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...-generation rAdV vectors. Created with BioRender.com. Second-generation rAdV vectors Second-generation...third-generation rAdV vectors. Figure 3: Second-generation rAdV vectors. Created with BioRender.com. Third-generation...Third-generation rAdV vectors and helper virus. Created with BioRender.com. Adenoviral Vector Production Cloning...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...

  2. Plan Your Experiment

    Type
    Guide
    ...Delivery method Expression System Components of System Application Mammalian expression vector Cas enzyme ...electroporation Transient expression of CRISPR components Expression decreases as RNA is degraded within...system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA expressed in your target...to the transient Cas9 expression. The table below summarizes the major expression systems and variables...details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP...gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing both Cas9 and ...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Guides AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...Plasmids required for rAAV vector production. Created with BioRender.com. AAV Vector Production Cloning Cloning...Addgene’s Viral Vector Protocols . Figure 3: Overview of rAAV vector production. Created with BioRender.com...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...used as viral vectors for gene therapy applications. This guide contains many viral vector-specific terms...adenovirus, read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral Vectors Vector Production...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols... Gamma-retroviral vector packaging methods. Created with BioRender.com. Viral Vector Integration Much ...long-term expression in a host and low immunogenicity. This guide contains many viral vector-specific ...Gamma-retroviral vectors do not have defined “generations” of plasmids, as lentiviral vectors do. For more...lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral Vectors The genome of gamma-retroviruses...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations Vector Production...Lentiviral Vectors Due to their integration and long-term expression of a transgene, lentiviral vectors are ...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...Overview of lentiviral vector production. Created with BioRender.com. Viral Vector Integration Genome-wide...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have ...models. Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host, and...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...

  6. Molecular Biology Reference

    Type
    Guide
    ...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors... protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian, yeast...process to stop. An expression vector can also include an enhancer sequence which increases the amount of protein... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA...combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule...

  7. Chemogenetics Guide

    Type
    Guide
    ...chemogenetic receptors. FLEx vectors block expression in the absence of Cre and are especially useful in AAV...tissue-specific Cre is provided. Want to know more about FLEx vectors? Read our Plasmids 101: FLEx Vectors blog!... Dlx Interneurons EF1a, CAG General expression Targeted expression. Depending on the experiment, an AAV-encoded...which they drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells... our AAV guide . FLEx Vectors are also used to achieve cell-specific expression of AAV-encoded chemogenetic.... The opsin may excite or inhibit the neurons expressing the LMO, depending on its biophysical properties...more than one domain, both of which need to be expressed. However, neuronal control through PSAMs and LMOs...

  8. Optogenetics Guide

    Type
    Guide
    ...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...fiber. Using viral vectors for opsin delivery results in more localized expression of the opsin. Depending...specific recombinase technology like Cre-lox, which allows you to express an opsin ONLY in a genetically defined...site of the viral vector, rather than in all cells. For example, using a viral vector with a floxed opsin...opsin in a VGAT-cre animal will result in expression of the opsin only in inhibitory neurons near the injection...adeno-associated viruses for Cre activated and inactivated transgene expression in neurons. Front Neural ...procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). When exposed to light of...

  9. CRISPR Guide

    Type
    Guide
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... gRNAs will be expressed in the same cell. This is called multiplexing , and increases the likelihood ...lentivirus or use a Cas9-expressing stable cell line. After applying your screening conditions to your transduced...also been used for genome-wide screens to activate or repress gene expression in mice and human cells. The...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...

  10. Cloning

    Type
    Guide
    ... TOPO® ready, and it is not feasible to create a TOPO® vector yourself. Additionally, the efficiency can...activity creates “chewed-back” overhangs of 10-12 base pairs on the 5' end of both the vector and insert...them into plasmids for replication and protein expression. So how do scientists recombine DNA? There are...majority of plasmids used for DNA cloning and expression contain several popular restriction enzyme sites...shuttled into any compatible Gateway® Destination vector, which contain attR sites via LR clonase enzymes...a simple and efficient cloning method, gateway vectors and recombination enzymes can be quite expensive...comes from a pre-cut, linear, cloning ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ ...

  11. Guide to Using Pooled Libraries

    Type
    Guide
    ...parsing the effects of expressing multiple genes at once. A well-designed screen can help you begin to ...information on genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our...given library have the same backbone, but they express or target different genes. Some libraries cover...Negative Screen Negative screens are a little trickier than positive screens. In a negative screen, the goal...plasmid. Library screens can be divided into two types: positive screens and negative screens. Both types ...reproducibility. Types of Pooled Library Screens In pooled library screens, cells are infected at a very low ... cells. DIVERGE: Positive and negative screens POSITIVE screen: Apply library Apply selection Most cells...

  12. Science Guides

    Type
    Guide
    ...Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune system, antibodies...given library have the same backbone, but they express or target different genes. Some libraries cover...restricted to certain gene sets. Read More Viral Vectors Find educational guides to learn more about the... Science Guides Addgene has created the following guides to help you start learning...
Showing: 1 - 12 of 12 results