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Addgene

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We narrowed to 5 results for: broad host range

Showing: 1 - 5 of 5 results

  1. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...considered to not integrate into the host genome. When wild-type AAVs enter a host cell, they uncoat in the nucleus...sites of the host genome can occur, although at a low frequency which has been estimated to range from 0.01%...serotype, and a very high infectivity across a broad range of cell types in vivo. AAV-DJ has been optimized...adenovirus or herpes simplex virus to replicate in the host cell and complete the lytic cycle. AAVs are therefore...establish latent infections by integrating into the host genome at a specific site, the AAVS1 site on human...proteins and are therefore unable to integrate in the host’s genome. Instead, the linear rAAV DNA is typically...avoids the effects local chromatin structures in the host genome can have on transgene expression and the ...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...to infected host cells. When these antibiotics are added to the growth medium of the host cells, they ...by their ability to integrate their genomes into hosts in order to continue their lifecycle and replicate...models, such as stable long-term expression in a host and low immunogenicity. This guide contains many...into DNA before being integrated in the genome of a host. Going from RNA to DNA is the reverse of what you...Gamma-Retroviral Vectors The genome of gamma-retroviruses ranges from 9–11 kb, encoded on RNA (Figure 1). This RNA...provirus, the cDNA that will be integrated into a host genome. This single strand of RNA contains packaging... anything in between will be integrated into the host genome. To produce gamma-retroviral vectors, these...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...to infected host cells. When these antibiotics are added to the growth medium of the host cells, they ...by their ability to integrate their genomes into hosts in order to continue their lifecycle and replicate...non-dividing cells, enable stable long-term expression in a host, and exhibit low immunogenicity. This guide contains...into DNA before being integrated in the genome of a host. Going from RNA to DNA is the reverse of what you...Lentiviral Vectors The genome of lentiviruses typically ranges from 8–10 kb, encoded on RNA (Figure 1). This RNA...provirus, the cDNA that will be integrated into a host genome. This single strand of RNA contains packaging... anything in between will be integrated into the host genome. To produce lentiviral vectors in the laboratory...

  4. Molecular Biology Reference

    Type
    Guide
    ...conjugation), usually providing a benefit to the host, such as antibiotic resistance. This benefit can...plasmid exists in a symbiotic relationship with the host cell. Like the bacterial chromosomal DNA, plasmid... providing a survival advantage to the bacterial host. Each bacterium can contain multiple copies of an... our popular Plasmids 101 series covers topics ranging from the newest breakthroughs in plasmid technologies...by scientists today come in many sizes and vary broadly in their functionality. In their simplest form,...

  5. CRISPR Guide

    Type
    Guide
    ... DNA, which scientists have modified for a wide range of genome engineering techniques . Collectively,... often found in plants, expanding the targeting range for plant genome engineering. Read more about Cas9...non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes TTTV (V = A.... R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum inhibitor of CRISPR-Cas9. Cell , 170 (6...& Liu, D. R. (2018). Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature...screens in E. coli identify essential genes and phage host factors. PLOS Genetics . 14 (11), e1007749. PMID...with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nature Biotechnology...
Showing: 1 - 5 of 5 results