We narrowed to 10 results for: car-t

...applications, like chimeric antigen receptor T cell (CAR-T) therapy, the location of integration can greatly...integration and its effect on clinical pre-infusion CAR T-cell products. Journal of Translational Medicine... M., Alvarado, A. F., Moffatt, T. N., Edavettal, J. M., Swaminathan, T. A., & Braun, S. E. (2021). HIV-based...doi.org/10.2174/1566523054546224 PMID: 16101513 Dull, T., Zufferey, R., Kelly, M., Mandel, R. J., Nguyen, ... 33981779 Kim, H. S., Hwang, G., Lee, H. K., Bae, T., Park, S., Kim, Y. J., Lee, S., Park, J., Bae, S.... Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench,..., Y., Liu, H., Lu, A., Ma, J., Cai, Y., Fuksenko, T., Pelayo, A., Shah, N. N., Kochenderfer, J. N., Norberg...

...disorders. Delivery of chimeric antigen receptor T cell (CAR-T) therapy is a particularly popular application...vector purification by high-speed centrifugation for CAR T cell manufacturing. Molecular Therapy — Methods ...options, including: Gibbon Ape Leukemia Virus (GALV) — T and B cell transduction Murine Leukemia Virus (MuLV...M. C., Varshney, G. K., Gildea, D. E., Wolfsberg, T. G., Baxevanis, A. D., & Burgess, S. M. (2014). MLV...doi.org/10.1093/nar/gkt1399 PMID: 24464997 Maetzig, T., Galla, M., Baum, C., & Schambach, A. (2011). Gammaretroviral...should be made on a case-by-case basis. Retroviruses carry a slightly elevated risk of integrating into proto-oncogenes...Naseer, A., De Ridder, J., Allahyar, A., Wang, W., Carpenter, E., Mason, A., Wong, G. K., Cameron, E. R., Kilbey...

...INTEGRATE ( I nsertion of T ransposable E lements by G uide R NA- A ssisted T argeting), developed by Samuel...Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M...PMID: 28041849 Sakuma, T., Nishikawa, A., Kume, S., Chayama, K., & Yamamoto, T. (2014). Multiplex genome... R. T., Silverstein, R. A., Amrani, N., Peng, C., Kramme, C., Savic, N., Pacesa, M., Rodríguez, T. C.,... (22), 5635-5652.e29. PMID: 34653350 Chu, V. T., Weber, T., Wefers, B., Wurst, W., Sander, S., Rajewsky... Gao, L., Cox, D. B. T., Yan, W. X., Manteiga, J. C., Schneider, M. W., Yamano, T., Nishimasu, H., Nureki...Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3 induces...

..., C, or T K G or T M A or C N A, T, C, or G R A or G S C or G V A, C, or G W A or T Y C or T Amino Acids...Guanine T Thymine U Uracil Single Letter Code: Ambiguous bases Nucleobase B C, G, or T D A, G, or T H A, ...A), Thymine (T), Cytosine (C) and Guanine (G). In the double helix, A always pairs with T and C always...thymine, cytosine, and guanine (abbreviated to A, T, C, and G, respectively). The bases are organized ...DNA form hydrogen bonds, where A always pairs with T, and C always pairs with G. The order of nucleotides...vitro DNA replication in addition to the normal A, T, C, or G nucleotides. The four standard bases (dNTPs... each time a base is added. Again, each base (A,C,T, or G) is labeled with a different color, making it...

..., X. P., Slocum, S. T., DiBerto, J. F., Xiong, Y., Urushihata, T., Hirabayashi, T., Fujimoto, A., Mimura...PMID: 25937170 Weston, M., Kaserer, T., Wu, A., Mouravlev, A., Carpenter, J. C., Snowball, A., Knauss, S....Prakash, M., Ikefuama, E. C., Friedman, N., Brown, T. M., Lipscombe, D., Moore, C. I., Hochgeschwender,...Farrell, M. S., Pei, Y., Wan, Y., Yadav, P. N., Daigle, T. L., Urban, D. J., Lee, H. M., Sciaky, N., Simmons...Sysa-Shah, P., Rodriguez, L. A., Ellis, R. J., Richie, C. T., Harvey, B. K., Dannals, R. F., Pomper, M. G., Bonci...Inoue, K. I., Kumata, K., Seki, C., … Minamimoto, T. (2020). Deschloroclozapine, a potent and selective...071013-014048 PMID: 25002280 Strader, C. D., Gaffney, T., Sugg, E. E., Candelore, M. R., Keys, R., Patchett...

...26289977 (Link opens in a new window) He, T. C., Zhou, S., da Costa, L. T., Yu, J., Kinzler, K. W., & Vogelstein... 21453277 (Link opens in a new window) Bulcha, J. T., Wang, Y., Ma, H., Tai, P. W. L., & Gao, G. (2021...Bullard, B. L., Corder, B. N., Gordon, D. N., Pierson, T. C., & Weaver, E. A. (2020). Characterization of a..., A. J., Gilbert, S. C., Hill, A. V. S., & Lambe, T. (2017). Chimpanzee adenoviral vectors as vaccines...: 29083948 (Link opens in a new window) Geisbert, T. W., Bailey, M., Hensley, L., Asiedu, C., Geisbert..., A., Lee, M. J., Wolf, J. M., Reid, R. R., & He, T. C. (2017). Adenovirus-mediated gene delivery: potential...Haydon, R. C., Kinzler, K. W., Vogelstein, B., & He, T. C. (2007). A protocol for rapid generation of recombinant...

...ChR2; increased photocurrent amplitude 450–470 C1V1(t/t) ChR1-VChR1 chimera with E122T and E162T mutations...F., Davidson, T. J., O'Shea, D. J., Sohal, V. S., Goshen, I., Finkelstein, J., Paz, J. T., Stehfest, K...-Benson, A., Cho, Y. K., Morimoto, T. K., Chuong, A. S., Carpenter, E. J., Tian, Z., Wang, J., Xie, Y...., V., Matthews, G. A., Acker, L. C., Sørensen, A. T., Young, A., Klapoetke, N. C., Henninger, M. A., Kodandaramaiah...Beltramo, R., Scanziani, M., Hegemann, P., Oertner, T. G., & Wiegert, J. S. (2015). An improved chloride-conducting...VChR1 Red-shifted channelrhodopsin from Volvox carteri 570 Chronos High-speed, red-shifted channel from...channel from Chlamydomonas noctigama ; ChrimsonR carries K176R mutation with increased on/off kinetics 590...

...group on the free 3' T. The topoisomerase acts as a ligase that joins the A and T compatible ends together...complementary base pairs adenine (A) and thymine (T). TOPO cloning utilizes the Taq polymerase, which ... on the 3' end of PCR products. The complementary T comes from a pre-cut, linear, cloning-ready TOPO vector...Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can be time-consuming compared...entire cloning step (digestion and ligation) can be carried out in one reaction with a single type IIS restriction... the final product, with no undesired sequence ("scar") retained. A disadvantage of type IIS assembly ...

...Link opens in a new window) Matsushita, T., Okada, T., Inaba, T., Mizukami, H., Ozawa, K., & Colosi, P....17763830 (Link opens in a new window) Liu, D., Li, T., Liu, L., Che, X., Li, X., Liu, C., & Wu, G. (2024...Tervo, D. G., Hwang, B. Y., Viswanathan, S., Gaj, T., Lavzin, M., Ritola, K. D., Lindo, S., Michael, S...Huang, C. C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A. W., Looger, L. L., Schaffer, D. V., ...viral (rAAV) vectors in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed... transgene of interest. The size of the genetic cargo that can be included between the ITRs is limited...for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins and...

...Marjanovic, N. D., Dionne, D., Burks, T., Raychowdhury, R., Adamson, B., Norman, T. M., Lander, E. S., Weissman...-0490-7 PMID: 29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA... producing stable cell lines, since the genetic cargo (in this case, CRISPR components) integrates into...