... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves... eat, drink, chew gum, or apply makeup in the laboratory. Before you start your experiment, make sure ...Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some times...officer. BSL-2 Guidelines Remember, the BSL-1 laboratory guidelines above are expected to be followed ...not be worn outside of the BSL-2 area. BSL-2 laboratories must be clearly marked as “BSL-2.” The names...names and contact information of the laboratory manager should be clearly visible in the room and on the ...vaccination or titer prior to starting work in the laboratory. For some biohazardous waste, an autoclave or...

...before putting the CS5 in the incubator. Return the CS5 to the incubator for 96 h. This incubation time...Hemocytometer or cell counter Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH ...pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T cells (...media and mix. Seed all cells in 1 CS2. Return to incubator for 48 h. Trypsinize and resuspend cells from ... with a total volume of 850 mL. Return to the incubator for 24 h–36 h. Proceed with transfection: Calculate...2% HI-FBS. Mix well. Take the CS5 out of the incubator and pour the media into a waste container. Carefully...evenly between the layers. Return the CS5 to the incubator for ~72 h. Harvest cells and media by tapping ...

...bacterial colonies. Equipment Shaking incubator at 37 °C Stationary incubator at 37 °C Water bath at 42 °C Ice...temperature and then (optional) incubate in 37°C incubator. Mix 1 - 5 μl of DNA (usually 10 pg - 100 ng) ...antibiotic) to the bacteria and grow in 37°C shaking incubator for 45 min. Pro-Tip This outgrowth step allows... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing...Class II, Type A2 Biological Safety Cabinet 4 °C Refrigerator Pipette controller Benchtop centrifuge compatible...tubes NanoDrop spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead...upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate filtered tissue culture supernatant...

...Harvest antibody Equipment Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes...conical tubes Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead... mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip ...swirl 5–10 times to mix. Return the flask to the incubator. Section 3: BCD Feed and valproic acid supplementation...

... the two, use a conversion calculator or chart like the G Force calculator (Link opens in a new window...Introduction A centrifuge is a common piece of laboratory equipment used to separate different components...

...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...

...pipette 200–1000 µL single channel pipette CO 2 incubator Pipet controller Hazardous waste container Microscope...orange/yellow if using a standard phenol red pH indicator) because the buildup of waste products could be...difficult to find. Leave the cells undisturbed in an incubator for 7–14 days. After ~7 days, scan the plate for...

...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents...time for a total of eight electroporations): Electroporator Conditions: Bio-Rad Micropulser Ec1 0.1 cm ...

...and bathroom. These items should remain in the laboratory. When traveling between lab spaces, take your...adhered to. None of the authors, contributors, administrators, or anyone else associated with Addgene, can...

...loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar plate (with appropriate...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...

...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...spreading Bunsen burner (or other small flame source) Incubator Reagents 6 cm diameter LB/agar plates without ...

...Addgene’s Mammalian RNAi Tools page. Several other laboratories have deposited pLKO derived vectors that may... temperature. g. Retrieve HEK-293T cells from incubator. The cells should be 50-80% confluent and in DMEM...should be decontaminated with at least 10% bleach. Laboratory materials that come in contact with viral particles...

... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to .... Water baths can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room...

... Protocols Video protocol guides for standard laboratory procedures. How-To Videos Screencapture walk-...Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization...

...and Two (BSL-1 and BSL-2) Safety measures for laboratories operating at BSL-1 and BSL-2 Watch the Video... its use Weighing Reagents Learn how to weigh laboratory materials on a balance Basic Molecular Biology...

.... Use a (Link opens in a new window) ligation calculator to easily quantify how much vector and insert...vector end. (Link opens in a new window) Ligation calculators are easily found on the web. Just enter the concentration...

...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents...Swirl gently to mix and place the dish in the incubator. Incubate for 48–72 h. Pro-Tip Different cells...

...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of...

...liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing liquid ensures the accuracy...Reagents Liquid for pipetting Background Information Anatomy of a Pipette This front view photo of a P1000 pipette...