...1) and biosafety level 2 (BSL-2). The BSL-1 classification is for labs working with low-risk microbes ...not be possible depending on the type of sleeve. Lastly, don’t touch your face! Close-toed shoes and long... be used as needed. For BSL-2 work always wear glasses/goggles in addition to the BSL-1 requirements. ...here has been designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene...

...good idea to place the gel on a glass plate if available. Unlike the plastic tray, this will not significantly...Gel You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis DNA Ligation Background...for your eyes!), remove it from any gel tray as plastic will block much of the UV and with a clean, sterile...

...media Day 4–5: Count fluorescent cells Equipment Class II, Type A2 Biological Safety Cabinet 0.5–10 µL ...obtaining accurate titers. Check the cells for mycoplasma (Link opens in a new window) regularly Do not...mCherry expression using fluorescence microscopy. Last reviewed on: September 9, 2023...

...Immunocytochemistry You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Western... intended to support reproducibility in science. Last Update: January 20, 2022 Workflow Timeline Day 1... the protein, such as cells transfected with a plasmid to express the protein of interest, and a negative...

...Stain cells and count resistant colonies Equipment Class II, Type A2 Biological Safety Cabinet 0.5–10 µL ...obtaining accurate titers. Check the cells for mycoplasma (Link opens in a new window) regularly Do not...single colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

...PCR) You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background...detailed instructions and check out our protocol on plasmid cloning by PCR to learn how to design primers for...

...Protein A or Protein G You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Transfection...following your institution’s hazardous waste procedures. Last Update: February 7, 2022 Protocol Video Watch this...antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological Safety Cabinet 4 °C Refrigerator...

...compliance with your institution’s biosafety regulations. Last Update: July 7, 2023 Workflow Timeline Day 1: Seed...harvest cells Day 4+: ddPCR and analysis Equipment Class II, Type A2 Biological Safety Cabinet Aspirating...green) and negative (black) droplets Sample ID Plasmid ID Dilution Copies/20 µL RRE (FAM) Copies/20 µL...

...Culture Recovering Plasmid DNA from Bacterial Culture Restriction Digest of Plasmid DNA Background Information...

... does not provide enough stability to hold the plasmid together through the transformation/replication...reaction is shown. Mix the components (add the T4 last) and incubate at room temperature for 30 minutes...

...Indirect ELISA Method You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibodies 101: ...plotted against the micrograms of antigen loaded. Last reviewed on: October 8, 2024...

... institution’s biosafety regulations. Equipment Class II, Type A2 Biological Safety Cabinet, Labconco ...on AAV titering using ddPCR, read our blog post. Last reviewed on: August 31, 2023...

...You may also like... Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer...

...Balance Scoopula or spatula Container (beaker, tube, flask, etc.) Reagents Material that will be weighed out...

... familiar with your lab’s instrument before use. Last Update: September 2022 Equipment Centrifuge Microfuge...

...correct pipette and pipette tip and using the pipette. Last Update: September 2022 Video Watch the video for...

...10.1089/hgtb.2015.051 (Link opens in a new window) . Last reviewed on: November 7, 2023...

...this blog post on RNA extraction without a kit . Last Update: April 10, 2020 Equipment Refrigerated microcentrifuge...