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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 35 results for: cat.3

Showing: 31 - 35 of 35 results

  1. Protocol - Bacterial Transformation

    Type
    Protocol
    ...transformation tube by placing the bottom 1/2 to 2/3 of the tube into a 42°C water bath for 30-60 secs ...bacteria are used as the means for both storing and replicating plasmids. Because of this, nearly all plasmids...expression) carry both a bacterial origin of replication and an antibiotic resistance gene for use as ...bacteria. Scientists have made many genetic modifications to create bacterial strains that can be more...plasmid DNA for the purposes of storage and amplification. Higher efficiency cells are more important ...

  2. Western Blot

    Type
    Protocol
    .... Boil the samples for 10 min at 100 °C . Section 3: SDS-PAGE Prepare the precast gel as follows: Remove...Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Introduction Western blot is a technique...lysis buffer will vary depending on the cellular location of the protein of interest. RIPA buffer is suitable...most proteins but more stringent buffers and a sonication step may be required for hard to extract proteins...BSA standard that range from 0–2000 µg/mL . In duplicate, dilute 10 µL of standard, blank, and lysate samples... nm . Calculate the average absorbance of the duplicate samples on the plate. Subtract the average absorbance...vary depending on the sample type and cellular location of the protein of interest. You may need to try...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...hand for 10 seconds. Incubate the sample(s) for 2-3 minutes on ice and centrifuge for 15 minutes at 12,000...You may also like... Kit-Free DNA Purification Agarose Gel Purification Molecular Biology Reference Introduction...information on nucleic acid quantification, see our protocol for DNA quantification , which can be modified... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

  4. Pouring LB Agar Plates

    Type
    Protocol
    ...on plates without any antibiotic. Negative Result 3: Only the Non-resistant Strain Grows If only the non-resistant...Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated, the antibiotic powder can be dissolved in dH ...test results. Sample Data In all cases below (-) indicates that the tested strain is not supposed to be resistant... resistant to the antibiotic, (+) indicates that the tested strain is supposed to be resistant to the ...

  5. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...stable cell pool were then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western...without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) Trypsin...orange/yellow if using a standard phenol red pH indicator) because the buildup of waste products could be...
Showing: 31 - 35 of 35 results