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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 40 results for: ANG

Showing: 21 - 40 of 40 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ... can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight. ...DNA will vary upon intended use. Typically yields range from several hundred micrograms to a milligram of...taken to perform adequate NGS based QC to ensure no change in representation compared to the pre-amplified...

  2. Weighing Reagents Protocol

    Type
    Protocol
    ...material that you are weighing by looking for a weight range on the scale. Make sure that the weight of the material... material you’re weighing out is within this range. If you need less than a gram of material, use an analytical...

  3. Protocol - Bacterial Transformation

    Type
    Protocol
    ...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific treatments...competent cells. Pro-Tips Commercial competent cells range significantly in their transformation efficiency...

  4. Immunocytochemistry

    Type
    Protocol
    ... antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL of the diluted antibody... antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL fluorescently-labeled...

  5. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...much as several miligrams and at concentrations ranging from 150 ng/μL to several μg/μL. The protocol below...pellet (can be done by inverting the tube at an angle over kimwipe) for 20-30 min. Resuspend pellet with...

  6. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...reverse primers are stable within this temperature range to anneal to each of the single stranded DNA template...polymerase that can withstand radical temperature changes during a typical PCR. The DNA polymerase has an...

  7. AAV Production in HEK293 Cells

    Type
    Protocol
    .... Optional: perform a media exchange at ~18 h post transfection. Changing the media can reduce toxicity...

  8. DNA Quantification

    Type
    Protocol
    ...measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample. Notes:...

  14. Fluorescence Titering Assay

    Type
    Protocol
    ...Data Figure 1: 293T cells were transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h ...

  16. General Transfection

    Type
    Protocol
    ... cells. Refer to the table below for a possible range of ratios to test: Ratio of DNA:PEI Amount of DNA...

  17. Lentivirus Production

    Type
    Protocol
    ... cells. Refer to the table below for a possible range of ratios to test: Ratio of DNA:PEI μg of DNA μL...

  20. Western Blot

    Type
    Protocol
    ...Prepare serial dilutions of the BSA standard that range from 0–2000 µg/mL . In duplicate, dilute 10 µL of...
Showing: 21 - 40 of 40 results