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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 36 results for: LUM;

Showing: 21 - 36 of 36 results

  1. Pouring LB Agar Plates

    Type
    Protocol
    ...200 mL of agar in 500 mL bottles. The extra empty volume is necessary to prevent your molten agar from boiling...Cover the opening of the bottle with its cap or aluminum foil (but do not make an air-tight seal!) and ...the first plate to get a good idea of what that volume looks like in your particular plate. For the remainder...

  2. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...loosely cover the entire top of the bottle with aluminum foil. Autoclave and allow to cool to room temperature... swirl. Loosely cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate ...

  3. Ligation Independent Cloning

    Type
    Protocol
    ...Inactivate T4 Pol by heating to 75° for 20 minutes. Volume (μl) Reagent Final Conc. 4 10X Buffer 1X 20-30 ...annealing reaction. This should be done in a small volume with no additional water (<5 μl). It is advisable...

  4. Centrifugation

    Type
    Protocol
    ...often an extra tube filled with water to the same volume as your samples. If your samples are significantly...need to fill the blank based on weight instead of volume. Pro-Tip For microfuge tubes, place them with the...

  6. Protocol - Bacterial Transformation

    Type
    Protocol
    ...you to recover all transformants. If the culture volume is too big, gently collect the cells by centrifugation...centrifugation and resuspend in a smaller volume of LB so that there isn't too much liquid media on the agar...

  7. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...of HCl at a time. Add deionized water to a final volume of 1 L and recheck pH to ensure that it has not...up to 4 times for a total of 16% of the culture volume. At 72-96 h post-transfection, supplement the flask...

  8. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... . Simply adjust the mass of agarose in a given volume to make gels of other agarose concentrations (e.g...sample you want to load on a gel, make 10% more volume than needed because several microliters can be ...

  9. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...to a microfuge tube. Add 8 µL of PBS to a total volume of 13 µL . Prepare the recombinant antibody Dilute... issues. Plot the mg/mL versus the HC + LC Area column for the IgG standards in Google Sheets. Open exported...

  10. DNA Quantification

    Type
    Protocol
    ...purity. Note: Purity is measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for...

  13. Weighing Reagents Protocol

    Type
    Protocol
    ... of reagent that will be resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may place...

  15. Lentivirus Production

    Type
    Protocol
    ...Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume 500 μL *Plasmid concentrations and ratios should...
Showing: 21 - 36 of 36 results