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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 42 results for: MAL

Showing: 21 - 40 of 42 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes prior...302411100 Droplet Digital PCR System, Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad...plate has been sealed, proceed to thermocycling. Thermal Cycling Run the following PCR parameters. Cycling...

  2. Weighing Reagents Protocol

    Type
    Protocol
    ...accuracy as these types of scales display more decimal places. Pro-Tip If you’re weighing out an amount...amount of reagent that will be resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may...

  3. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...time (approximately 18-30 hr). On the other hand, smaller plasmids can be present in large numbers, 50 or...may help to increase the density of the culture. Normally cultures shake at 150 - 250 rpm, increase this...

  4. Ligation Independent Cloning

    Type
    Protocol
    ...sterile water (instead of TE buffer) to ensure optimal salt concentrations in subsequent reactions. Step...per annealing reaction. This should be done in a small volume with no additional water (<5 μl). It is advisable...

  5. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...the bacteria back into suspension, but within a smaller volume of buffer that is compatible with the next... genomic DNA to precipitate, while leaving the smaller plasmids free in solution. Pellet the proteins ...

  6. Pouring LB Agar Plates

    Type
    Protocol
    ...~220 mL) just in case we spill anything or have small errors in measurement. You should also always make...-mix slightly. Even so, you should always use thermally insulated gloves when removing anything from the...

  12. Centrifugation

    Type
    Protocol
    ...height. They often spin at similar speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges...

  15. Lentivirus Production

    Type
    Protocol
    ...discard the tube and thaw a new working stock. The optimal mass DNA:mass PEI ratio will need to be empirically...
Showing: 21 - 40 of 42 results