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  1. Ligation Independent Cloning

    Type
    Protocol
    ...Independent Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols...time-consuming ligation step of traditional cloning methods. In traditional cloning, base-pairing in the short...

  3. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... we list the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and...bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant antibody plasmid...

  5. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...two 75 cm 2 flasks, etc. Pro-Tip This selection method results in a polyclonal cell population, meaning...screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4 (Link opens in a new window...

  6. AAV ddPCR Titration

    Type
    Protocol
    ...reached, place the PCR plate with the foil onto the metal support block. Place the block in the plate sealer...thermocycling. Thermal Cycling Run the following PCR parameters. Cycling Step Temperature (°C) Time (min) Ramp...

  7. Kit Free RNA Extraction

    Type
    Protocol
    ...the quality of your RNA sample(s) using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer...

  9. Protocol - How to Streak a Plate

    Type
    Protocol
    ...often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to check this before...

  12. General Transfection

    Type
    Protocol
    ... Introduction This protocol describes a general method for transfecting mammalian cells using linear polyethylenimine...

  15. Lentivirus Production

    Type
    Protocol
    ...incubator Pipet controller Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high ...

  20. Pouring LB Agar Plates

    Type
    Protocol
    ...right temperature, we recommend using a laser thermometer. Light the flame at the plate pouring station...
Showing: 21 - 40 of 41 results