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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 30 results for: abo.1

Showing: 21 - 30 of 30 results

  1. Protocol - How to Streak a Plate

    Type
    Protocol
    ...of the plate, as shown in the diagram above, to create streak #1. Pro-Tips Hold your tooth pick at an ... or freshly sterilized loop, drag through streak #1 and spread the bacteria over a second section of the...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...

  2. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million bp depending on the polymerase...recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each varies,...cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent cells...Background In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same ...

  3. Using a Light Microscope Protocol

    Type
    Protocol
    ...Figure 1 depicts an image of a compound light microscope with the main components labeled: Figure 1: Diagram...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of...

  4. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...annealing can be achieved by one of two methods: Method #1 Place the mixed oligos in a 1.5mL microfuge tube. ...vector in molar ratios (vector:insert) between 4:3 and 1:6 in a standard ligation reaction (ex. to ligate an...water and quantify the concentration (should be about 8ng/μl). Mix the annealed oligos with cut vector...

  5. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each varies,...cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent cells...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...window) New England Biolabs for more information about restriction enzyme buffers). If you select enzymes...

  6. Weighing Reagents Protocol

    Type
    Protocol
    ...resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may place a microcentrifuge tube on the balance...balance in the photo above has a capacity of 200 g, as indicated by the number above the "Zero" button. ... reagents for a stock mixture is an essential laboratory technique, as imprecise measurements can affect...

  7. Water Bath Protocol

    Type
    Protocol
    ...quicker. Pro-Tip Set up the water bath 30 minutes to 1 hour before you need to use it to allow the temperature... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to ...even if you are using disinfectants as described above in step 3. You will also need to maintain the appropriate...

  8. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...insert, but significantly off center (ideally around 1/3 of the way from one end), and also cuts in the backbone...verification, such as DNA sequencing . In the example above, digestion with enzyme RE1 will linearize the 6200bp...that you get double confirmation. In the example above, digestion with either RE3 or RE4 will give a very...

  9. Gibson Assembly Protocol

    Type
    Protocol
    ...ssDNA. (Rabe & Cepko, 2020). Incubate the mix for 1 hour at 50 °C or follow manufacturer's instructions... the right). When designing your plasmid, think about what DNA segments you will need to join to create...

  10. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...solution, add 2-2.5 volumes 95% or 100% ethanol and 1/10 volume of 3 M Na-acetate (pH 4.8). Invert the microfuge... supernatant out of the tube if you are worried about losing the pellet. Dry with vacuum or by inverting...
Showing: 21 - 30 of 30 results