We narrowed to 24 results for: ids

1. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ... a new one. When developing the assay multiple plasmids containing ITR were tested. Plasmid #59462 is ... Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs have reported...

2. AAV Production in HEK293 Cells

   Type

   Protocol

   ...protocol, but their conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid... the total number of base pairs from all three plasmids, we can determine the total μg/bp we need to achieve...

3. Water Bath Protocol

   Type

   Protocol

   ...as ethanol. Writing on the tops of the tubes and lids of items to be placed in the water bath is often...

4. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

   ...contaminants with lower densities, including empty capsids. The 60% step serves as a cushion for genome-containing...