We narrowed to 34 results for: mme

1. Western Blot

   Type

   Protocol

   ...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...a clean microcentrifuge tube. Use the lysate immediately or store at -80 °C until ready to use. Section...

2. Antibody Validation Using the Indirect ELISA Method

   Type

   Protocol

   ...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...wash, aspirate the wash buffer from the wells. Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide...

3. Protocol - How to Create a Bacterial Glycerol Stock

   Type

   Protocol

   ...100% glycerol in dH20. Snap top tubes are not recommended as they can open unexpectedly at -80°C. Freeze...

4. Protocol - How to Streak a Plate

   Type

   Protocol

   ...setting is important for organization, and it is recommended that you keep a standard labeling system for ...

5. Protocol - How to Purify DNA from an Agarose Gel

   Type

   Protocol

   ...from the gel. This is most commonly done with a commercial gel purification kit, such as the (Link opens...

6. Protocol - How to Inoculate a Bacterial Culture

   Type

   Protocol

   ...Antibiotic Concentrations Commonly Used Antibiotics Recommended Concentration Ampicillin 100 µg/mL Bleocin 5 ...

7. Centrifugation

   Type

   Protocol

   ... unbalanced or improperly loaded centrifuge. Immediately stop the centrifuge and investigate the issue...

8. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

   Type

   Protocol

   ...Kit Extract RNA from tissues or cells without a commercial kit Plasmid Cloning Name Description (Link opens...

9. Transfection for Recombinant Antibodies

   Type

   Protocol

   ...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...

10. What is Polymerase Chain Reaction (PCR)

    Type

    Protocol

    ...385µl of water. After making your 100uM stock, immediately make a working concentration of each primer (...

11. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...efficiency will vary between cell lines. It is not recommended that lentiviral supernatants be subjected to ...

12. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...

13. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type

    Protocol

    ... was used for the initial selection). Addgene recommends that the use of selective medium throughout experiments...

14. AAV Production in HEK293 Cells

    Type

    Protocol

    ...Pro-Tip Stirring during the cooling period is recommended or the solution may separate into phases. Aliquot...