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Showing: 21 - 40 of 45 results

  1. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...use one or the other, but there is very little difference between the two. Note: Make sure to use the same... buffer as the one in the gel box (do not mix different buffers and do not use water). Microwave for 1...be in the top portion and your bands will be differentially intense. If this happens, you can just soak...to load 1.0 μg in 10 μL, make 1.1 μg in 11 μL. Reference Page Top Index...

  2. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...plasmid Resuspension buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated..., including plasmid DNA, into solution. Add a renaturing solution to the denatured bacteria. Note: This...Solution II at room temperature Solution III - Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium...included in the resuspension buffer of most kits. Reference Page Top Index...

  3. Western Blot

    Type
    Protocol
    ...SDS-PAGE gel tank. Pro-Tip Different SDS-PAGE gel tanks will have different gel orientations. Refer to...may need to be adjusted to accommodate slight differences between products. Addgene does not endorse or... Inclusion of this information is solely for transparency intended to support reproducibility in science...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol describes how to use...1814040 Microseal adhesive seal, Bio-Rad, MSB1001 Polystyrene Reservoirs, VWR, 89094-662 Microcentrifuge tubes... the NTC. Pour the 1X dilution buffer into a polystyrene reagent reservoir. Using a 20–200 µL multichannel...cartridge. Add 800 µL of droplet generation oil to a polystyrene reagent reservoir. Using the 20–200 µL multichannel...

  5. Video Library

    Type
    Protocol
    ...career path, differences in research culture between the US and Australia, and advice for current graduate...Specialist Jane Hannon discusses her career path, current projects in the works, and all of the things she...

  6. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... at specific sequences. There are hundreds of different restriction enzymes, allowing scientists to target...(such as a digestion with another enzyme in a different buffer), but will not be gel purifying it, you... not cut your insert, you could try using two different enzymes that have compatible sticky ends. See...

  7. General Transfection

    Type
    Protocol
    ... This approach can be adapted for different cell lines and different transfection reagents. Workflow Timeline...5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS can promote or inhibit ...

  8. Immunocytochemistry

    Type
    Protocol
    ...method. The protocol may need to be optimized for different cells, target proteins, etc. Sharing speeds science...may need to be adjusted to accommodate slight differences between products. Addgene does not endorse or... Inclusion of this information is solely for transparency intended to support reproducibility in science...

  9. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ... to use, you can check out our Empty Backbone Reference . Background Subcloning by restriction digest ... a fusion protein) Ideally, you will find two different restriction enzymes for your subcloning. It is...expression vector and can begin your studies. Reference Page Top Index...

  10. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...for pipetting error. In this example, we have 7 different reactions (7 unique primer pairs), so we multiply...sequence. FAQ How do I design primers? See our reference page on how to design primers for detailed instructions...environment for the DNA amplification reaction. Reference Page Top Index...

  12. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Sample Data Selection of E.coli on LB-agar using different concentrations of carbenicillin plated over-agar...over-agar Plate shows a lawn of E. coli and no apparent selection. 150 µL of 1 mg/mL Carbenicillin plated...

  13. Water Bath Protocol

    Type
    Protocol
    ... biology, and thaw cell lines. There are many different types of water baths that exist as scientists ...scientists work with tubes and bottles of different sizes in the lab. Some water baths contain individual slots...

  14. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ... of the four levels is to distinguish between different types of hazards and to provide protective measures...measures for each level, as each level has different safety requirements. BSL-1 is designated for those...

  15. Lentivirus Production

    Type
    Protocol
    ...21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter, Nalgene...and 5 mL of glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS can promote or inhibit ...

  16. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...may need to be adjusted to accommodate slight differences between products. Addgene does not endorse or... Inclusion of this information is solely for transparency intended to support reproducibility in science...

  18. Kit Free RNA Extraction

    Type
    Protocol
    ...Purification Agarose Gel Purification Molecular Biology Reference Introduction This protocol describes how to extract...ThermoFisher Scientific . Figure 1: A diagram of the different steps in RNA extraction. Before Starting RNA is...
Showing: 21 - 40 of 45 results