We narrowed to 38 results for: nes

1. Transfection for Recombinant Antibodies

   Type

   Protocol

   ...Tuesday (120 h post-transfection): Add 4% BCD Feed. Wednesday (144 h post-transfection): Add 4% BCD Feed. Thursday...

2. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

   ...SYPRO Ruby stain to determine purity. Only the cleanest fractions should be kept and pooled for dialysis...

3. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

   Type

   Protocol

   ... comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder standard...

4. What is Polymerase Chain Reaction (PCR)

   Type

   Protocol

   ...cations can also be used for PCR-mediated DNA mutagenesis. A higher cation concentration increases the ...

5. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ... 3125000X Dilutions highlighted in green are the ones loaded onto the qPCR plate for most samples. If ...

6. Pouring LB Agar Plates

   Type

   Protocol

   ...Plasmids can carry one or more antibiotic resistance genes, which confer resistance to a specific antibiotic...

7. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ... comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder standard...

8. Lentivirus ddPCR Titration

   Type

   Protocol

   ...Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...