AAV Purification by Iodixanol Gradient Ultracentrifugation
Type
Protocol
.... 0.1% Poloxamer 188 in PBS (A) Add 500 µL of 10% Poloxamer 188 to 49.5 mL PBS 0.01% Poloxamer 188 in ...Stocks of Poloxamer 188 Poloxamer 188, 10% solution A: 0.1% in PBS: 49.5 mL PBS + 500 µl Poloxamer 188 B:... Hamilton 1X PBS pH 7.4 1X PBS-MK buffer 100X Poloxamer 188 NaCl MgCl 2 KCl Centrifugal filter units (... (B) Add 5 mL of Buffer A to 45 mL PBS 0.001% Poloxamer 188 in PBS + 200 mM NaCl (C) (formulation buffer... Cover the filter membrane with 15 mL of 0.1% Poloxamer 188 PBS and incubate for 10 min at RT Spin at ... discard the flow through. Add 15 mL of 0.01% Poloxamer 188 PBS and incubate for 10 min at RT. Spin at...discard the flow through. Add 15 mL of 0.001% Poloxamer 188 + 200mM NaCl PBS. Spin at 3000 rpm for 5 min...