...potential splice site mutations. The CRISPResso suite accommodates single or pooled amplicon deep sequencing... you'll need Sanger sequencing reads from PCR amplicons that cover your locus of interest and correspond... from a deep sequencing experiment a reference amplicon sequence to assess and quantify the efficiency...efficiency of the targeted mutagenesis The amplicon sequence expected after HDR can be provided as an optional...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Description Gene/Insert Antibiotic Resistance PI Publication Back to Top Do you have suggestions for other...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions for other plasmids that...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI... Plasmid Gene/Insert Vector Type Promoter PI Publication Back to Top Do you have suggestions for other...

...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...

...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

...signaling network for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential...classification and treatment. On each pathway page, click on a component to find plasmids with your gene of... described in the initial Target Accelerator publications, focused on somatic variants found in lung adenocarcinoma...

...Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert PI Publication Plant ID Plasmid Gene...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Gene/Insert PI Publication Do you have suggestions for other plasmids that should be added to this list?...

...Marker PI Publication Bacteria Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...together because they are intended for use as a set. Click here to view all kits. Kit Types Fluorescent Proteins... backbone and only differing in a small region. Click here to view all pooled libraries. Pooled Library...plasmids from a depositor organized by a common use. Click here to view all collections. Depositor Collection...

... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger...

...RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link...plasmids! To get started with your deposit, please click here ....