TALENs for Endogenous Zebrafish Gene Targets
Recent reports have shown that engineered TALENs can be used to induce targeted mutations with high efficiency in zebrafish (Sander et al., Nat Biotechnol. 2011; Huang et al., Nat Biotechnol. 2011). The table below lists pairs of TALENs that have been engineered by the Joung, Peterson, and Yeh labs at MGH to target sites within various endogenous zebrafish genes; many of these were generated as part of an NIH-sponsored effort to provide gene-editing nucleases to the zebrafish community.
| Target Gene Name | TALENs | Full Target Site (5' to 3'; half-sites in CAPS, spacer in lowercase) |
|---|---|---|
| aak1 | TAL3208 & TAL3209 | TCTTTGACTCCCGGAGAGagctggtgggctccgggCCAGGTTCTGGAGGGGGA |
| abcg2a | TAL3400 & TAL3401 | TGTCCTCATCCCCGTCCCgccgcggcgccaccgtCAGCTTCCACAACATCAA |
| adam15 | TAL3016 & TAL3017 | TGTTTCTGGCGGACTCTCgtcaccattcttctgtTTGAAGGCACACCTTTGA |
| ahcyl1 | TAL3018 & TAL3019 | TGACAGACTGTGGCGAGGatgccaagctggaggcGACGAAACAGGCCAGCAA |
| amh | TAL3210 & TAL3211 | TCCAGGCAAGATTTGGGCtgatgctgatgatgacCGTGGCGATTGGGTCGTA |
| Aminopeptidase-o | TAL3020 & TAL3021 | TGATCTGGATCCTAACAAggatgacttgcccctgtGTGCGAACACCAACCATA |
| arf6 | TAL3212 & TAL3213 | TGGGGAAGATGCTTTCCAagatcttcggcaacaaGGAGATGAGAATATTGA |
| arnt1 | TAL3214 & TAL3215 | TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA |
| apoa4 | TAL3022 & TAL3025 | TGGCAGGACCAACCAATGcccagcatggacctggTGAAAAATGCTTTCTGGA |
| apoa4-like | TAL3023 & TAL3024 | TGAAGGTTCTTGTGGTGCtcacacttgctgtggtTACAGGTAAGAAACTAAA |
| apoeb | TAL3402 & TAL3403 | TGCCAGGCTCGTAGCCTGttccaggctgatgccccTCAGCCCAGATGGGAGGA |
| arap3a | TAL3026 & TAL3027 | TGGCAACAATCGGTCCGTgctcagatgcagaggaCCTGTTGGCTTCCATCCA |
| ARSA | TAL 3002 & TAL 3003 | TCAAGAGGCGGTCTCCCTctcaacgagaccaccaTAGCCGAGGTGCTGAAGA |
| asun | TAL3404 & TAL3405 | TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA |
| atf6 | TAL3406 & TAL3407 | TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA |
| atg5 | TAL3218 & TAL3219 | TAAAGTCAAGAAACATTTtctcaaagtcatgaagGCAGAGGATGTGGAGGAA |
| atg12 | TAL3216 & TAL3217 | TTAATCAGTCGTTTGCGCcatcaccggaccaagaAGTTGGTGTGCTTTTTGA |
| avpl | TAL3220 & TAL3221 | TGCTGTGTGTGCTCGCGCtctccacgctctcgtcTGCCTGCTACATCCAGAA |
| avpr1b | TAL3028 & TAL3029 | TCACGGCGAACAACACTAccgatccgttcgggagGAACGAAGATGTGGCCAA |
| bbc3-(puma) | TAL3030 & TAL3031 | TGGCACGCCGAACAGCTGcaggatggaggtgctgCGTCAGGACGCCTGGCCA |
| bmp10 | TAL3032 & TAL3033 | TCAGCTCCCCGGAGAGGCaccgcactgctccagggTTGGATGATGGACATGGA |
| bsx | TAL3034 & TAL3035 | TCTCAGACTCACAGTTATccggactcgagaaaagGTTCGAGATCCAGAGATA |
| btr30 | TAL3036 & TAL3037 | TGGACGCGGAGCTGTTCTcggagcaggagctgacCTGCTCCATCTGTCTGGA |
| casp8ap2 | TAL3038 & TAL3039 | TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA |
| Ccl2 | TAL3222 & TAL3223 | TTTTCGTTTTCCACTATAaagatcccagttaagcAGGTTCAGAGTTATCATA |
| cebpa | TAL3040 & TAL3041 | TATTCCACAACAGCTCCAagcaagagaagctcaaACTGGCGAGCGGAGACTA |
| cebpb | TAL3224 & TAL3225 | TGGAAGTGGCCGGTTTTTacgagggggactacctCGCTTTCCACAGCACTAA |
| cebpd | TAL3042 & TAL3043 | TGACTTCTGCTGCGTCCAtgtctgacatgtacaatCTGGACTCGCAGTGCGTA |
| CHCHD4 | TAL3408 & TAL3409 | TCTACCTAATGGCGACATaaactggaactgcccgtGTTTGGGCGGCATGGCCA |
| cish.b-(socs8) | TAL3044 & TAL3045 | TTCTCAAAATGAGAGGGGtgaatctgaagttcaaCAAACCCAGCAGCACCAA |
| CIT1 | TAL3226 & TAL3227 | TGAAGTTTAAATATGGAGgtcatggcagtgttaaAGATCTGTCCGCAGTCGA |
| cited2 | TAL3228 & TAL3229 | TGGTAGACCGCATGATGGcaatgaaccatggtcgTTTCCCAGATGCTGTGAA |
| cited4a | TAL3046 & TAL3047 | TGAACCATGGTTCTGCTGgcggaggcctgcatggTTATCGCATGGGAATGAA |
| cited4b | TAL3048 & TAL3049 | TGAACCACGGCCAGGGAAacgggggtctgcatggCTACCGGATGGGCATGAA |
| clcn1a | TAL3050 & TAL3051 | TCCTCTGTTCTCAGCTATatggagagtacagagaGCACCCTGGTGGGTCTCA |
| clcn1b | TAL3052 & TAL3053 | TTTTATTGTGTTACAGACtgcctagcccgtgtccAGAGGTATATTGTGACAA |
| col22A1 | TAL3410 & TAL3411 | TGGATGGCGAGAACAGGGcggtggagtataacgcTGTGGGCCTGACGAAGGA |
| Complement Factor H | TAL3230 & TAL3231 | TGATTACATGTGAACCTCcatcacgtgaccagcatgTGTCCACCTCCTTTTGGA |
| cln3 | TAL3054 & TAL3055 | TGTGTCTTCAGGTCGTTGtcaacgctggaggaatTGTGTTGCGTTCTGGTAA |
| crispld2 | TAL3232 & TAL3233 | TATCAGGAGGAGCTAGAAcccaacagcactaaacCCGACGCTCCTGTCCGAA |
| cx52.6 | TAL3056 & TAL3057 | TGGGATGATGAGCAAAGTgagtttgtgtgcaacaCAGAACAGCCTGGATGCA |
| cx52.7 | TAL3058 & TAL3059 | TGGGTGGACGAGCAGAGCgagttcgtctgcaacaCGGACCAGCCTGGATGCA |
| Cyp19a1 | TAL3412 & TAL3413 | TGCTCCAGCCCTGTGGAAtgaagccggtgcgtctCGGCGAGGCTGTGGTGGA |
| CYP20A1 | TAL3414 & TAL3415 | TAGACTTTCCTGAAAAGCaaaaatgctagattttGCCATTTTTGCTGTGACA |
| dachc | TAL3234 & TAL3235 | TCCAGACCTGGCAGACCTcctaaaaggacccagaATGTGACATCACCCGACA |
| dazl | TAL3060 & TAL3061 | TATGAAGGTGGATGAGAAcgagatcagggaattcTTTGCCAAGTATGGCTCA |
| dbx1b | TAL3236 & TAL3237 | TCCCGTGCAAGGAATGAAtgcaaaaacatttcctTTCCCTTGTTTTGACGGA |
| dcps | TAL3416 & TAL3417 | TGAACAGTCCACCGATAAcccagtggtagacttcCAGCTCACCCGCATTCTA |
| ddx26b | TAL3418 & TAL3419 | TGAAGGCCAGCACGACACtcacctgcgtcaacctCTTCGTCATGCCTTACAA |
| def6a | TAL3420 & TAL3421 | TCAGAACTGCTCAAGTCCatttggtacgctttcaCAGCTCTGGATGTGGAGA |
| DGKE | TAL3238 & TAL3239 | TATGGTGTCAAACCACAGtgcatgatgactgtctTTCCAGTCTGACCGATGA |
| Dlk1 (site #1) | TAL3240 & TAL3241 | TGGTGGCGCAGCAGAGGGagctgatccaggaccaGGCCACCGTGAACATCA |
| DLK1 (site #2) | TAL3422 & TAL3423 | TGAGGTACAGGCAGCTGGtggcgcagcagagggagcTGATCCAGGACCAGGCCA |
| Dlk2 | TAL3242 & TAL3243 | TTGGCCACGGAAAATGTGccgaaaatggcgactgCAGGTGTGATCCGGGA |
| drd4a | TAL 3006 & TAL 3007 | TCAATATGACCGTATGTGatggactgatgaccatgGATGTCATGCTTTGCA |
| drd4b | TAL3244 & TAL3245 | TTCACTTACAATGTTCCCgcgctcgtgtttgggaTATTGCTTATCATTGTTA |
| dyrk1aa | TAL3424 & TAL3425 | TGTTCCGAAACCACCTGTgtctggtgttcgagatGCTGTCCTATAACCTGTA |
| dyrk1b | TAL3426 & TAL3427 | TCCTGAACCAGGCCCAGAtcgagctgcggcttctTGAGCTCATGAACAAACA |
| ednrb1b | TAL3062 & TAL3063 | TACATCAGCAGCGTGGTGtcgtgtgtggtgttccTGCTCGGCATGCTGGGAA |
| egf-like-domain | TAL3064 & TAL3065 | TTGGTGATGTCTGGAGTCgtcgcgtgtcttacagCACAGAGTCTTTTCTTCA |
| eif2a | TAL3428 & TAL3429 | TCACCTACAGAAAGTGTCtgagtttgctctgtctcCTGGATCACAGCCGAGCA |
| elk1 | TAL3066 & TAL3067 | TTACATTGTGGCAGTTCCtgcttcacctcctggaTGATCAGAATCAGAAGCA |
| eml2 | TAL3068 & TAL3069 | TCAGAAAAGTGGGAAGACggatttgaggacaggcGTTTTAAAATAGGGAGAA |
| EMP2 | TAL3246 & TAL3247 | TCAGGCAGTGCAGGCCACcatgatactggccaccATCTTGTGCTGTGTGGGA |
| eomesb | TAL3430 & TAL3431 | TCTTCTCACCCGGAGGCGgaggaggagaaagtttcTCCGGTAGTTTGTGGTGA |
| epd | TAL3432 & TAL3433 | TGGGCTTCTCATCACTCTcatcggcagccatgtcATTCACCACAGCTGACCA |
| eps15 | TAL3248 & TAL3249 | TTGCCCATTGCCTATCGCaacgcaggatgaactaGTGGGAACAGAAACACTA |
| esr1 | TAL3250 & TAL3251 | TCAGTGGGATACTACCCGgctcccccagacccccACGAGGAACATCTACAGA |
| ets1a | TAL3070 & TAL3071 | TCAGAAGAGGAACATCAGcacactccagatctggTGGAGAAAACACAAACAA |
| f11r | TAL3434 & TAL3435 | TTGACTTGCAATGTTCCTacacttctgactttggAGCAACACCCAGAGTAGA |
| fabp3 | TAL3436 & TAL3437 | TGTTTGTCTTCTGTTGTTtgtattcagtctgttaTAACTTTAGATGGAGGGA |
| fam123b | TAL3252 & TAL3253 | TGGAGATCGCCACAAGATgtgaggttggtgcaatGAGGGGTCCATCGTCAGA |
| fan1 | TAL3072 & TAL3073 | TTGCCTTATTATCTGCGAaactttcggactgtccTTGAGGCTGTGTTGGAGA |
| fascin-2b | TAL3074 & TAL3075 | TGCCCTCCAATGGCAGCAaagcccttaagcttcaATTCGGGCTTATTAATCA |
| fastkd3 | TAL3438 & TAL3439 | TACCTGTCTTCTAATGAGgaccacgcttttatgaAACGCCTCTCCTCTTGTA |
| fgfbp1a | TAL3254 & TAL3255 | TGTCTCTCCGCGGAATTTtcgcgttcctgctcttTCTCGCGTGCGTCTCACA |
| FoxF2a | TAL3440 & TAL3441 | TCAGGTCCAGCCCGGCATccaacagcatgcactcGGCTCTCCAAAACACGCA |
| foxl2 | TAL3256 & TAL3257 | TAACGGCATGATACTCATggacacaacctcgagtTCAGCGGAGAAAGACCGA |
| foxp3a | TAL3260 & TAL3261 | TGCTTCTTAATGCTACTGgaacacacagaggggaTGATAACAGGAGTAGTCA |
| FoxP1b | TAL3258 & TAL3259 | TCGGGGACAGAGGCGGCAaacggaacggcccatcAGAACGGCGCTCCGCCCA |
| Frizzled 5 | TAL3262 & TAL3263 | TCGGATTTTGGCTGCATGtcctgctgctgtttcaACTGTCTGGGCTCGGAGA |
| Fryl | TAL3264 & TAL3265 | TTCCAGGCGGTGCGTAAGaagttcatcacagagcTGAAGGAACTGAGGCTGA |
| fscn 2a | TAL3266 & TAL3267 | TAAATCACGAAAATCGATacctaactgcagaggcATTTGGTTTCAAGATCAA |
| fshr | TAL3442 & TAL3443 | TGTTGTCAATGATGCTGTgctttattcttggctgCTCCATGGCAAACACCGA |
| gabra4 | TAL3076 & TAL3077 | TAAAGAGAATATCTGGACagatcagaaaggatgaGAAATTATACCCGGAAAA |
| gabra5 | TAL 3014 & TAL 3015 | TGATAACATCACAGTATTcacacgaatccttgacGGATTGCTGGATGGCTA |
| gabrg2 | TAL3078 & TAL3079 | TGGAAAGCGATGATGACGaagtgacaaacaaaacATGGGTTCTGACTCCCAA |
| gata2b | TAL3080 & TAL3081 | TCAATGTGTCGACCTCACcttatcaacactcacgGTCTTCCTTTACTGGACA |
| gbas | TAL3082 & TAL3083 | TACCGGCAGCACAACCGTgaggccgtcggtggattCTTCTCTCAGATCGGCGA |
| GDF2/BMP9 | TAL 3010 & TAL 3011 | TTGAACAAGGTGGAGAGTttcttaggctttatgaAGGAAGATTTTTTGAGGA |
| ghsra | TAL3084 & TAL3085 | TCTCTCAGCATGCCTACCtggacgaaccggtccaACTGTTCCTTCAACTGCA |
| ghsrb | TAL3086 & TAL3087 | TGGACAAACGTGTCCATCtgcccgctcagcatcaCTTTATGCGCTGAGAACA |
| gna13a | TAL3088 & TAL3089 | TTCCTGCCCTCCCGGACTgctatagtttgtattcCCAATTGTCTTCTCTCCA |
| gna13b | TAL3090 & TAL3091 | TCCTGCAGTCAGGGTCCTttctatccctttgtttTCCAAACTGTTTTCTCGA |
| GnIH | TAL3268 & TAL3269 | TGTAAGATGTCCTACTTCgctcttctttctttagCCCTCGGCATCCTGAGCA |
| GnRH3 | TAL3270 & TAL3271 | TTGGAGGTCAGTCTTTGCcagcactggtcatacgGTTGGCTTCCCGGTGGAA |
| gopc | TAL3092 & TAL3093 | TCACCGGGATCGGGCCTCtccgccggcccggctgCGGCTACCGGCTCCGGTA |
| gper | TAL3272 & TAL3273 | TGGAGGAGCAGACTACCAatgtgattcagatttaTGTGAATGGCACCGAGCA |
| gria3a | TALEN1258 & TALEN1260 | TCGTCCAATAGCTTCTcagtcacgcacgcctgtGAGTTTCTGCTCTTTA |
| gria3a | TALEN1258 & TALEN1259 | TCGTCCAATAGCTTCTcagtcacgcacgcctgTGAGTTTCTGCTCTTTA |
| gria3a | TALEN1295 & TALEN1260 | TCGTCCAATAGCTTCTCagtcacgcacgcctgtGAGTTTCTGCTCTTTA |
| GRIN2B | TAL3444 & TAL3445 | TCCAGGTGTACCCGTACCccgtgaggccgacggagtGCGCCACCTGGAAGATGA |
| GRM2b | TAL3446 & TAL3447 | TATCTTGCCCGGCTTGAAattgggagcccatataCTGGACACCTGCTCGAAA |
| hcrt | TAL3448 & TAL3449 | TACAAGACAGGGGACAGAagctaaactcatggactgCACAGCTAAGGTAAGA |
| hemogen (site #1) | TAL3274 & TAL3275 | TGGAAGACCCGTTGGAGAaagagatcccaccaacTGAAATAAAAGATTCAGA |
| hemogen (site #2) | TAL3276 & TAL3277 | TGATTTGTTTGTTTGCTaggaggaattcggcggCGACTCAGAGACAGAGA |
| her6 | TAL3094 & TAL3095 | TGACAGCGTTTACTCCACtagaagaagctgtgcgGGAGGATTTCTTTTTATA |
| hey2 | TALEN1297 & TALEN1257 | TCTTCCGTTTCCACATCCaccacatcccaacagagcAGCGGGAGCAGCAGTAAA |
| hif1al (site #1) | TAL3278 & TAL3279 | TGTTTCAGCAGAGCCCCGctgaagagctccccatGGAGATGGAAGGAGTGGA |
| hif1al (site #2) | TAL3280 & TAL3281 | TGCCCTCAGGACTTCTGCacgcctgaactccgcaAGCTTCTGTCTCCAATA |
| hirip3 | TAL3282 & TAL3283 | TGGCCAAAGAAAAGGACGcgataagaaagtttgtTGTGGCTCAACTGCAGAA |
| hist2h3ca1 | TAL3284 & TAL3285 | TCTGTTTGAGGACACCAAcctgtgcgccatccacgCCAAGAGGGTCACCATCA |
| hnrnpk | TAL3096 & TAL3097 | TCAGCAACAAAGAGAGTAatggtaagatctagtaaAAAATCTACATAAATGGA |
| hnrnpr | TAL3286 & TAL3287 | TGGATGCGGGTCTGCCCCagaaggtggccgagagCCTGGATAACATCTTCCA |
| hnrnpu | TAL3288 & TAL3289 | TTAAGGTGAACGAGCTGAaggacgagctaaagaaGCGGCAGCTCTCTGACAA |
| hs3st1 | TAL3292 & TAL3293 | TCAATCCTCTGTCCTGGTgtctggcgtcatctctCCGCCTGATGCTGCGCTA |
| hs3st1l1 | TAL3290 & TAL3291 | TCTTCTGAGTTATGTGTTattcctggcgctccgcGCATACGCCGCTCCTCCA |
| hs3st3l | TAL3450 & TAL3451 | TCACCTGCATCTCCATCCtctaccttctgctgggATGCTGCGACTCCTCCGA |
| hsd17b1 | TAL3452 & TAL3453 | TTGTTCTCATCACTGGATgctcttcagggattggACTCAGCCTTGCTGTGCA |
| hsd17b4 | TAL3098 & TAL3099 | TCTGGGTGGAGATATTAAaggtggaggcaagagcTCAGCGGCTGCTGATAAA |
| Hsf1 | TAL3454 & TAL3455 | TCACCGGGAACAATGTGCctgcgtttctgaccaaACTCTGGACGCTGGTGGA |
| id2a | TAL3100 & TAL3101 | TAACGACGACGGAGCACAgtctgggaatctcgcgGAGCAAGACCCCCGTGGA |
| Id4 | TAL3294 & TAL3295 | TTTATTACAATGAAGGCCagcgtgccggttcgccCTCATAAGCTTCCTTCTA |
| igf3 | TAL3456 & TAL3457 | TGCCAGACAACACTGAAGgggccagagcacgctgCGGACGAGAACTAGTGGA |
| igfbp1a | TAL3458 & TAL3459 | TGCTTGCCGAAACCGGGAgaagcccgacccctgcaCGCGCTCACCCGGGGACA |
| igfbp1b | TAL3460 & TAL3461 | TCCGGGATCCGGGCTGCGgctgctgttcctcctgCGCCCTGCGGAGCGGCGA |
| Impact homolog (mouse) | TAL3462 & TAL3463 | TGGACAAATTAGAGGATCatgacgataataatctGCAGTCTCAGATAGAGGA |
| INF2 | TAL3464 & TAL3465 | TAAACGCCCTGATCTTCAgcgccgacggcctgcagcAGAGAGACAAGATGCGCA |
| inhibitor of DNA binding 3 | TAL3466 & TAL3467 | TCAACATTGGATATCTATagagctttacagctagTTTATCCCAGTGTCCAGA |
| IPIP27A | TAL3296 & TAL3297 | TGAACGAGAGGAGTGTGGcacactatgccacatgCAACTCTCCTCCAGACAA |
| ippk | TAL3102 & TAL3103 | TCCTCAACATTATGGACTatagcaaacatgtcatGAAGCCTTTGCTTGGGGA |
| irf6 | TAL3104 & TAL3105 | TCTCATCCACGGCGTGTGcgtctgaagccctggcTGGTCTCTCAGGTGGACA |
| jarid2a | TAL3298 & TAL3299 | TGGGATCCCGTGGTCAGAagagagagtggtccgcCGGGTGCTCTACCTCTCA |
| kcnma1a | TAL3468 & TAL3469 | TGCCTGGCACAGGGCCTGtccaccatgctcgccaacCTCTTCTCCATGAGGTCA |
| kcnma1b | TAL3470 & TAL3471 | TGCCTGGCACAGGGGCTctccaccatgctggccAACCTGTTCTCAATGA |
| kinesin-family-member-13a | TAL3106 & TAL3107 | TGTTCTCTGTTTGAGCGAgtctccacacagcagaGCGACAGTAACAGCTTCA |
| KISS1RA | TAL3472 & TAL3473 | TTTTCTGGTGAAGACTGGaattctagtgagcttcTGAATGGCTCTTTCAGAA |
| KISS1RB | TAL3474 & TAL3475 | TGATGGCAGAAACTAACAgcaccggcgatgctgcAGAGCACATCATGTGCAA |
| kiss2 | TAL3108 & TAL3109 | TCGACTAGCACGCAGTAAattcaactacaatccgTTTGGGCTGCGATTCGGA |
| klf4a | TAL3478 & TAL3479 | TGTCTCACCTGAAACGCCcctgcatgtctacaggTGCGACCTGCGCTGACCA |
| klf8 | TAL3476 & TAL3477 | TGAGCCCACAGACCTCAAaatcaagtcctcgcctcCACCGCTCTGCATCATCA |
| LBH | TAL3480 & TAL3481 | TGTGCTCGTCTTCAGATCttcccggacccgtcagACTTCGAGCGCTGCTGTA |
| lhx1a | TAL3300 & TAL3301 | TTTATCGGACAAAGAAACgggtaacaatgaaaatGATGACCAGAACCTTGGA |
| lrrtm1 | TAL3302 & TAL3303 | TCTAATTGGTCTGTACCTaaagtggccgctaaggAAGCCCCCTGGGCTGATA |
| lycat | TAL3482 & TAL3483 | TCCTTCATCTTCATCCAGcggcgctgggaggacgACCGCACACACATGAGCA |
| lyl1 | TAL3110 & TAL3111 | TCTTTCCTCATGCCGCCGgcaggattcgggatctTCAGTAACGCTCGTATGA |
| mak | TAL3304 & TAL3305 | TCAAGCAGCTGGGTGATGgaacctatggcagcgtTCTAATGGGGAAGAGCAA |
| mapk14a | TAL3306 & TAL3307 | TCTTCTTGTGCTCTTATTggcaggtacctggtgacTCACCTTATGGGCGCTGA |
| matrixmetallopeptidase15 (membrane-inserted) | TAL3484 & TAL3485 | TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA |
| matrixmetalloproteinase14a (membrane-inserted) | TAL3486 & TAL3487 | TGTTCCTGGTGGCGGTGCatgaattgggtcatgcaCTTGGTTTGGAGCACTCA |
| max | TAL3308 & TAL3309 | TGAGCGACAACGATGAtatcgaggtcgacagtGATGTGAGTATAGTTA |
| mchr1a | TAL3310 & TAL3311 | TGGCCATCTGTTTGGCCTttttcatctgttgggcTCCATATTACATCCTTCA |
| MCHR1B | TAL 3008 & TAL 3009 | TGTGCACTGTTATCACAGcgctggactctaacagCCAGATCGTGAGCACTTA |
| mcu | TAL3112 & TAL3113 | TGCGAAAGTGTGTAGATCggtcctcctcctgtccCGGAGCAGCGGAGCCGTA |
| mdm2 | TAL3114 & TAL3115 | TTATGTTTTATTTAGGGAaatatatcatgagcaaGGAGTTATATGACAAGCA |
| mef2aa | TAL3312 & TAL3313 | TGGGACGGAAGAAGATACagatcacgcggatcatGGATGAGAGGAACCGGCA |
| mef2d | TAL3488 & TAL3489 | TGGGGAGGAAAAAGATCCagattcagcgaatcacAGACGAGCGTAACAGACA |
| meis1 | TAL3116 & TAL3117 | TGTACGGGGACCCGCACGccgccagatccatgcaGGCTGTTCACCTGAATCA |
| meis2a | TAL3314 & TAL3315 | TGAGCTGGCTCACTACGGcggcatggacggggtcGGGATGTACGGGGATCCA |
| meis2b | TAL3490 & TAL3491 | TGCTGGCGCTGGTGTTTGagaagtgcgagctggcCACATGCACACCGAGAGA |
| meis3 | TAL3118 & TAL3119 | TTGGTGCAGTACCCAGGCtctgacgccatgcctgTGGGAGGCTACAGGGACA |
| meis4.1a | TAL3316 & TAL3317 | TACGAGGATCTGGTCCACtacggcatggatgcggTCGGGATCCCGTCTCCGA |
| mical2b | TAL3492 & TAL3493 | TGGGGGAGACGGAGGAAGaacggacatctcaggcCGGTCAGCTTTTTGAGAA |
| mir-726 | TAL3120 & TAL3121 | TCAGTCGTATGCCTGGAAttccgctagttctgaaCTATTCGTGATTGGCAAA |
| mrc1a | TAL3318 & TAL3319 | TGTTGTACAAGGGATCAGgtatatggagtcgctgGCAGGTGTATGGGACCAA |
| mtdhb | TAL3494 & TAL3495 | TGCCAGCACAATAGATTGcaatggcaccgggttgGCAAGAGATCGCGTCGCA |
| musashi-2a | TAL3122 & TAL3123 | TGGAAGCGGACGCGAGTCaagtcacctctggaagCCTGAACGATTCGCAACA |
| mycn | TAL3124 & TAL3125 | TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA |
| NADPH oxidase, EF-hand calcium binding domain 5 | TAL3496 & TAL3497 | TGCCGCCAACTGGCTGAAacctccagatctggagCAGAACAAGCGCAAAA |
| Neurl1a | TAL3498 & TAL3499 | TCTCTAGGTGGACCATTTccctcaacagctcaccGATGCCATCACAAGCCCA |
| nphs2 | TAL3126 & TAL3129 | TTCACACAGAACCATACTgaaatcggtaagaaaaGGATGACCACTGACAGGA |
| NPHP5/iqcb1 | TAL3500 & TAL3501 | TGGTCTGGCTGCTTTCAGagacatcgaggtgttcAGTCATGAATTTCTGCCA |
| nptx2a | TAL3128 & TAL3127 | TCACCTTTTTTCGTTTGGaagaacgaataagtcgtgAGAGCTTTCTTTGGGGTA |
| NPVF | TAL3320 & TAL3321 | TTAGCCCTCGGCATCCTGagcagcttcatgctaaGTGAAGTTACGGCTCTCA |
| NR1I2 | TAL3502 & TAL3503 | TCGGAGCTCGCCGTGTCTgctcgtgatgaggcagtACGTCAATGTCCCGCTTA |
| nr2e3 | TAL3130 & TAL3131 | TCCTGAACACGGGACTTCtttgtaaagtgtgttcTGACACCAGCAGTGGGAA |
| nr5a2 | TAL3322 & TAL3323 | TGGACTACTCCTACGACGaagacttggatgagatGTGTCCAGTGTGCGGAGA |
| nup155 | TAL3324 & TAL3325 | TCGCTGTCTGGCGTGTCCgacatggattatcctcTGCAGGGTCCCGCGCTGA |
| NUPL2 | TAL3326 & TAL3327 | TGGATAAACCCATCTCAGcgtggtggcggcggctCCTCGTCAGCAGGGGGCA |
| olig3 | TAL3132 & TAL3133 | TTCCACGTGTTCCTTGTAacaaggattgtgaaatATGACATTTCAGATGAAA |
| ovol1 | TAL3328 & TAL3329 | TGTTCCAGTTCCAGCGCAtgctaaaccgacacctGAAATGTCACAGCGAGCA |
| oxtr | TAL3330 & TAL3331 | TTCAAGGATCAAGACTTTtggtcctttaatgagtCATCCAGAAACGCCACCA |
| oxtrl | TAL3332 & TAL3333 | TGATCCTCTGGCCCATTAacgactcctgggccaaCTCAAGTAGGGGAAACGA |
| Park2 (site #1) | TAL 3012 & TAL 3013 | TGGAGCAGGGTGCGAGTGtgtctgagctgaaggaGGCGGTGGGTCGTTTACA |
| park2 (site #2) | TAL3334 & TAL3335 | TGATCGTTTTCGTGCGGTttaattccagccatggGTTCCCGGTGGAGTTGGA |
| parn | TAL3134 & TAL3135 | TCCCATACCTAACTCAGGaggaggagtgtcagctGAGGGAGCAGTATGAGGA |
| patz1 | TAL3136 & TAL3137 | TGGAGAGGACAGAGCAGCcgtggaattcctcctaCACGTACCAGGTGAGCAA |
| pax1a | TAL3504 & TAL3505 | TGAAAAACATACGGGACTacaaacaaagtgacccGGGAATTTTTGCATGGGA |
| pax1b | TAL3506 & TAL3507 | TGGGAATCAGACCCTGCGacatcagtcgacaactCAGGGTCTCTCATGGA |
| pax7a | TAL3508 & TAL3509 | TTAGAAGTCTCAACACCTctgggtcaaggtcgagTCAATCAACTCGGTGGA |
| pbx2 | TAL3510 & TAL3511 | TGGAGCAGACCCCGCGGGcctcgaaggcacagaaaACGGACACGACAACCGCA |
| pcdh10a | TAL3512 & TAL3513 | TCGCCGAAGATCTGGGGCtggacatcaccaaactTTCCGCGCGCCGTTTCCA |
| pcolce2b | TAL3336 & TAL3337 | TCCTCACATTTCGTTTCCtggatttagagagtgaCAACCTGTGTCGGTACGA |
| per2 | TAL3138 & TAL3139 | TCAGCACTACTGGTGTCAtcgggaagcaacaagaGGTAATTTGTGATTTTCA |
| pex2 | TAL3140 & TAL3141 | TGACAAGCCTTTCGCCAAggccggcccaagccctTTATCTCGGGTTTTGAGA |
| pink1 | TAL3338 & TAL3339 | TTTCCTCTCATAGTCAATattaattctctatttgGCTCAAAATGTCAGTAAA |
| Plekho1b-1 | TAL3142 & TAL3143 | TCGTCAAAGCGGGGCCCTcaggatgccaatcaacAGCCTGTGCAGCCCGACA |
| pls1 | TAL3514 & TAL3515 | TACGGGAGATTGTAGAGAagttcattgccggtgaCACCAACAAAGATGAGAA |
| Popdc2 | TAL3340 & TAL3341 | TGAATTGGAGACAGCAGAggctcaaacatgaataGCGGCGACTCTTCATTGA |
| Popdc3 | TAL3342 & TAL3343 | TCTCACTGTTAAGATTATtcataatccacaatggATCTTTTACCTGAGAATA |
| Prdx3 | TAL3516 & TAL3517 | TGCTTTCACGCATTGCACtacacattggcaagatgGCAGCCACCATCGGGAGA |
| prothymosin alpha a | TAL3344 & TAL3345 | TTATCATTCGCATCTCGTatttctctttatattaTTTTATTCCGAGACCCCA |
| prp19 | TAL3518 & TAL3519 | TTCCAGTTTCAAACGAAGtgccggagcacccctgTGTGTCCCCTGTTTCCAA |
| prrx1a | TAL3520 & TAL3521 | TGGGTTTCGTCAATTTGCgtcatgacttccagctATGCTCATGTAATGGACA |
| prrx1b | TAL3346 & TAL3347 | TAATACACTTGCCAATTTttatcgtatttgcgtcATGACTTCTAGCTATGCA |
| ptp4a3 | TAL 3000 & TAL 3001 | TCTCCTGAAAAGGCGCTTcatcgaggagcccggcTGCTGCGTGGCTGTGCA |
| pum1 | TAL3144 & TAL3145 | TCATGGTGCAGCCGTTACtggacagccgcgttccCTACGGACGCCTCCAGCA |
| Purinergic receptor Y2 | TAL3348 & TAL3349 | TTCAACGCATCAGTGAATgttcaaaaccacagttCTGCACACAGCTGCAGCA |
| pycr1 | TAL3146 & TAL3147 | TAAAGAGACCGTGAGCAAgagtgacgtccttttccTGGCGGTCAAGCCTCATA |
| rasa1a | TAL3148 & TAL3149 | TGAGGGGCGAGGACAGGGgatcggacggcagcagAGGCCCCGACTCGACCCA |
| rasa1b | TAL3150 & TAL3151 | TGGAGGCAAGGAGCCTGAtcccttttcaatggtgCCCCGCATAACCAGAGCA |
| Rasd1-201 | TAL3152 & TAL3153 | TTTGACATCCCGGCGAAAaactgctaccggatggTGATCCTCGGGTCCACCA |
| rassf7b | TAL3350 & TAL3351 | TGAAGGTGTGGGTGGATGgcattccccgtgtcgtCTGTGGCCTCTCAGAAGA |
| rbfox1l | TAL3154 & TAL3155 | TGTCTTCTCCTACTGTGAtccttcagccctacggACTCCCAGTTTACCCTCA |
| rbfox2 | TAL3522 & TAL3523 | TGAGGAGGGCGCAGTGGTcccgttcttcccccctCCTCCTCCTCCTCCTCCA |
| rbp4 | TAL3524 & TAL3525 | TGTTAAGGCTCTGTATAGcagtgtgtgtcctggcCACTTGCTGGGCTCAGGA |
| retinitis pigmentosa 2 (X-linked recessive) | TAL3526 & TAL3527 | TCTTTTTGTGCTGCGCCAcccagcccataatcgaGTCTTCTACAGGCATGAA |
| retinitis pigmentosa GTPase regulator b | TAL3528 & TAL3529 | TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA |
| retinitis pigmentosa GTPase regulator b ORF15 | TAL3530 & TAL3531 | TAAGAGGGCGGAGCTTTTatctcgcaaagctctgCCCACTGAGTTACTTAGA |
| rps19 | TAL3156 & TAL3157 | TGGTGTAACAGTGAAAGAcgtcaaccagcaggagTTTGTCCGGGCGCTGGCA |
| ryr2b | TAL3158 & TAL3159 | TATCTGCAGCCTTTCCCGtgtgtttcttggagctGAGTGACCTTCATTCTCA |
| scoc-a | TAL3160 & TAL3161 | TTTATGAGTCTTGTTTTCtcctcttgctcaacttGATTCTCCATATCTCCTA |
| Scoc-b | TAL3162 & TAL3163 | TATGAGTCTGGTCTTCTCctccaactccacttgaTTTTCTATGTCACCTGGA |
| scrt2 | TAL3352 & TAL3353 | TTTTCTGGTGCGTCTGTGcgaccgtaaaccatgcCTCGCTCGTTTCTGGTGA |
| sdc4 | TAL3532 & TAL3533 | TGGAGTCCTCCGGCAACTcacagaatgaagacttTGAGTTCACCGACTTAAA |
| sdcbp | TAL3534 & TAL3535 | TCTCCCCGAACCTTCCAGtgaccagaatgaacccAGTACCTACTATGATTCA |
| sdccag8 | TAL3536 & TAL3537 | TGGTTCCCATCATAAACAaccagtctgagtacatTCAGCACCTGGAGGCTGA |
| sema6dl | TAL3354 & TAL3355 | TGCTGATACTCCTGCTGGtggcgtcccgtctggaTGCTGTGAGCTTTCCTGA |
| Semaphorin 6bb | TAL3356 & TAL3357 | TTGAAAAGTGCTGAGAAAtgtgatgtcattggagTTTGATTTAGGAGACAAA |
| si:ch211-120j21.1 | TAL3358 & TAL3359 | TGAACTGTCAGTCGGGACtgcgatggttggtaacTTTATGCGCTTTCTTTCA |
| si:dkey-208k22.3 | TAL3538 & TAL3539 | TTATTCTCAGGCCAACTTtggcttggctgccaagAAGATGCACAAGTCGAAA |
| six4a | TAL3540 & TAL3541 | TTTGTTTGCGCACAGTTCgcccgcagtaaccgcgACCTCTTTGGCTTTCTCA |
| slc17a9a | TAL3542 & TAL3543 | TCTCCATTGGTCACGGATggccatgccgatctgtGCCGTCACCATGGCTAGA |
| slc17a9b | TAL3544 & TAL3545 | TGCTCTACTGTGCGCGTGtggccatgcctatctgTGCCGTGAGCATGGCGGA |
| slc26a6 | TAL3164 & TAL3165 | TAATTTGAGTGAAGTATTtaagagaataaaaaagCATTTGTAAAATCGAGAA |
| slc50a1 | TAL3360 & TAL3361 | TTTCTGCAGCTGCTATCAtgtgcgtgcatcatatTCACCGTCGGGATGTTCA |
| slit3 | TAL3546 & TAL3547 | TGGACAGGAATAACATCAcccggatcaccaaagtGGACTTCTCTGGCCTCAA |
| slmapb | TAL3548 & TAL3549 | TGGAGGCCAGTGAGTCTGagaaggtcatccagcgGCTGAACCAGGAACTGCA |
| smad2 | TAL3166 & TAL3167 | TAGCTTACTATGAACTTAaccagcgggttggagaAACATTCCACGCCTCTCA |
| smad3b | TAL3168 & TAL3169 | TTTCATACTATGAGCTGAatcagcgtgtcggtgaGACTTTCCATGCCTCACA |
| smad9 | TAL3170 & TAL3171 | TCTCCTTCACCAGTCCGGctgtgaagcgtctgctCGGCTGGAAGCAAGGAGA |
| smyd1a | TAL3550 & TAL3551 | TGGAGGTGTTCGGGGCCGggctgaagggccggggTCTGCGGGGTACCAGAGA |
| snai2 | TAL3172 & TAL3173 | TGCCTCGTTCATTCCTAGtaaagaagcatttcaaTGCAGCTAAGAAACCGAA |
| snai1b | TAL3552 & TAL3553 | TGAAGTTTCGAGGGGATAtttacaaagatgccacGCTCATTTCTTGTCAAGA |
| snrnp35 | TAL3362 & TAL3363 | TCCGGTGGCGAAGGTGTAcgaccctctgaaagcgGGCAGCATTGACGGCACA |
| snrnp48 | TAL3364 & TAL3365 | TGTGTGAAACATCGTTTGtttcgggtctccaggaGCGCATGCAGTGTCTTCA |
| socs3b | TAL3174 & TAL3175 | TGCGCAAACTGCAGGAGAgtggcttctactggagCACCGTGAGCGGGAAGGA |
| Solute-carrier-family-16 | TAL3176 & TAL3177 | TTGTGTGTGTGTGTGTGTgtcagtgagcagcagcTCGCCACAGTGTTGGAGA |
| sox1a | TAL3366 & TAL3367 | TATAGCATGATGATGGAAacggaccttcattcccCGGGACCCCAAACCAACA |
| sox2 (site #1) | TAL3178 & TAL3179 | TGGAAACCGAGCTGAAGCccccggcgccccagcccaACACCGGGGGCACGGGGA |
| Sox2 (site #2) | TAL3368 & TAL3369 | TGGTGGGGTAGACTTTCGagaaaatcggtttaaaTGTATAACATGATGGAAA |
| sox9b | TAL3180 & TAL3181 | TTATGGTTTGGGCTCAAGccgcgcggaggaaactGGCGGACCAGTACCCGCA |
| sperm associated antigen 7 | TAL3554 & TAL3555 | TTCTGGGCTCGATCTTGAgctcgatggaaaaaccTCCAACCGTTCACGATCA |
| spry4 | TAL3182 & TAL3183 | TCATGGTGCAGCCGTTACtggacagccgcgttccCTACGGACGCCTCCAGCA |
| sqstm1 | TAL3556 & TAL3557 | TGTCGATGACAGTGAAAGcttaccttatcggtaaGGAGGACTGCAACAAGGA |
| srf | TAL3558 & TAL3559 | TGGACGCTCTGGTAATGGcacgggattgacacctCTACAGGTGGGCAGCACA |
| stac | TAL3184 & TAL3185 | TGCAGAGGCTAAAGCGTTcgctgtccttcaagacCAAGAGTCTTCGCAGCAA |
| Stat3 | TAL3560 & TAL3561 | TGCAGCAGTTGGAGACGCggtatctggagcagctGTATCACCTGTACAGCGA |
| stxbp1a | TAL3562 & TAL3563 | TCAGCTTGCCCTGACCCCctgtttaatgaagtggTGAAGAGCCGTGCCTCCA |
| Sun1 | TAL3564 & TAL3565 | TGGATTTTTCCCGTCTGCacacgtacacccctccTCACTGTACTCCGGATAA |
| svila | TAL3186 & TAL3187 | TCGATAATGATGTTCTCCaaagtcttccaggttgTGGTGGTCTAGTTTCTAA |
| swap70b | TAL3566 & TAL3567 | TAAGGGACGAGCTTCTCaaggcaatctggcatgCCTTCACAGCCCTGGA |
| SYNE2 | TAL3568 & TAL3569 | TATGGGATGAGCTGGACCagtggcacactcggatAGCTGAGTTAGAGGGCGA |
| tac3a | TAL3188 & TAL3189 | TCAGCAGTCAGAGTCTCAaagatcagtttcaagcGTAAGTGACAGATTATGA |
| Tac3b | TAL3370 & TAL3371 | TCCACGTGCTGCTGCTGCtcgcgtgcccgagactCTCGCGGAGCGCCCTCGA |
| taok2b | TAL3372 & TAL3373 | TGCGGGCTGGGAGTCTCAaggacccggaggtggcGGAACTTTTTTACAAGGA |
| tbx4 | TAL3570 & TAL3571 | TGCTTCAGGAGAAGGCCTcagttgtggctgatgaAGGAATGACCGTTGCCCA |
| tcof | TAL3374 & TAL3375 | TAAAGACTCCTCCACCAGccaaacctgcggccccTGCAGTGTCCAGTAGTGA |
| tes | TAL3190 & TAL3191 | TTGAGGACCATGACGTGCagctggagactgaagaGAGCAAGAAAGTTGGGAA |
| tet2(ENSDART00000113020) | TAL3376 & TAL3377 | TCAAGACCAGATACTATCcccaagcttcctttccCAGCTACCACCTACTGAA |
| tet3(ENSDART00000137355) | TAL3378 & TAL3379 | TTGCTCCTTGGTTGCATCtctgtcttcattatcaCAAACATTTTCTTCATTA |
| tetmethylcytosinedioxygenase 1 | TAL3572 & TAL3573 | TCAACTCGCGCATCCACAaagcgaaatgttaagaGGGTGAAGGCTTCCATGA |
| tetmethylcytosinedioxygenase 3 | TAL3574 & TAL3575 | TTATTAGGCACTTTTTTGtaaacgctgtattccaTGCACTTTCCTGTCCACA |
| tetmethylcytosinedioxygenase 3 (previous si:ch211-220a11.1) | TAL3576 & TAL3577 | TCAAATCCTGAATCTCAAcaaggtgtttggagtgGTCAAAATGCCAGCACAA |
| tfe3a | TAL3380 & TAL3381 | TAAAATAGCCAGTCAGAcactggcaacgtcgacCCCACCACAGCCCTCA |
| tfe3b | TAL3382 & TAL3383 | TCGCTCAGCTCCGCCCTgcagacaccactcctgccATCTCCGTCTCGCTGCCA |
| tfeb | TAL3192 & TAL3193 | TTGGATGTGTACACGGGGcccggtatgtctggatCTGCAATAGCCATGACCA |
| tfec | TAL3194 & TAL3195 | TTTCCGATGGATGAAGTTattgacgatcttattgGCCTTGAAAACGGGTTTA |
| TIMM22 | TAL3578 & TAL3579 | TTGTTCTGGGAGGTGCTtttggagtcttcacagCCGGCATTGATGCAAA |
| tmem88 | TAL3196 & TAL3197 | TTCCACGAAACGGTACTTtggagaagctcgcgtcCCTGCAGACCCCAGCGGA |
| tnfb | TAL3384 & TAL3385 | TGTCGGGGGAGTTTATCAgacaaccgtggcacctGTACCGGTTAATTCTTCA |
| tppp3 | TAL3580 & TAL3581 | TGAGGGTTCGGGTGAAATatcgctgggagaggttGAGATGGCATTTCGTAAA |
| TRABD2A | TAL3386 & TAL3387 | TCAGCTGGAGCGCTTTTCtgcagatctgctggatACTCATGGTGAGAGCAAA |
| trappc11 | TAL3582 & TAL3583 | TCCTATGAGTGGTACATTcctaaagggattctgaAGACGGGCTGGATGAACA |
| TTC19 | TAL3388 & TAL3389 | TGCCAGGAGGAGCAGGGAgagtcccatccgcagacGCTGGTCCTGATGAGTGA |
| ucma | TAL3584 & TAL3585 | TGCTGATCGCGCTGACTGgggttgaaagtgcagcTGTTAAAGATGGAAAA |
| ulk2 | TAL3198 & TAL3199 | TGAGGGTCTTTCTACAGCagattgcagccgccatGCGAATCCTTAACAGCAA |
| v2rl1 (Q4VDB3) | TAL 3004 & TAL 3005 | TTACGAAGGACGCTTTTTgttggttttatgcatgATTTCTTACCTTTTCACA |
| Vangl1 | TAL3390 & TAL3391 | TTCTCTGCCCAGGATAGcagtgtttcgtgccctGCTGGGTTTACTGACA |
| vasa | TAL3392 & TAL3393 | TGACTGGGAGGAAGATCAgagtcccgttgtgtctTGCAGCTCAGGATTTGGA |
| Vascular-endothelial growth-factor-aa (vegfaa) | TAL3200 & TAL3201 | TCTCGCTTTGCTTCCTCCtcagtgattcccttcaTGGATGTGTATAAAAAGA |
| vclbAL772263.1 | TAL3586 & TAL3587 | TGGCCCAGCAGATCTCCCatctggtgatcatgcaCGAGGAGGGAGAGGTGGA |
| Vrk2 | TAL3394 & TAL3395 | TGTACTTGGCTGACTATGgtctctcatacagataCTGTCCTAATGGCGAGCA |
| ybx1 | TAL3588 & TAL3589 | TGAGCAGCGAGGCCGAGAcacaacagccgccgcaGCCTGCCGCCGACGCGGA |
| yjefn3 | TAL3590 & TAL3591 | TTGAGACGGAGCTCCTGAgggactaccgcttcggCCAGCAGCAGCTGATTGA |
| ywhai | TAL3396 & TAL3397 | TACAAGAATGTGGTGGGAgctcgccggtctgcctGGAGGGTAGTGTCCAGCA |
| zbtb7b | TAL3202 & TAL3203 | TGCGGGACGTCCTGAAGGgggcgcagctgctgggCATCCAGTGTGTGGCCGA |
| zgc-193690 | TAL3204 & TAL3205 | TCCTGAACAAGCTGATCAgaagcaatctggtcaaTAACACAAACCAGGTGGA |
| zmynd11 | TAL3398 & TAL3399 | TATGTTTGGTCGGCCATCgaagtcattcgcaatcAGAAACAAATCGCCAACA |
| znf385c | TAL3206 & TAL3207 | TCACAACATGCTGATCGGtacggctgctcaggggAGCCCCCTGCTGGCCTCA |