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(Plasmid #19993)


Item Catalog # Description Quantity Price (USD)
Plasmid 19993 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5524
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Neurofibromatosis type I
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    NF1 (a.k.a. NFNS, VRNF, WSS)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site kpnI (not destroyed)
  • 3′ cloning site PvuII (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The authors note that there is a mutation in the coding sequence: C3918T

"This created a T to I change within the GAP domain of NF1. However, this
change is outside of the conserved blocks within the GAP domain. This
particular residue (1195 of NF1-GRD shown in figure 4 in the chapter by
M.R. Kim and F. Tamanoi, chapter 6 in Neurofibromatosis type I from
genotype to phenotype, editor by M. Upadhyaya and D. N. Cooper BIOS
scientific publisher) is not conserved among different GAP proteins.
Therefore, we believe that the amino acid change does not affect the GAP
activity. In fact, we have used this construct for the wild-type GAP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT9-NF1-GRD was a gift from Fuyuhiko Tamanoi (Addgene plasmid # 19993 ; ; RRID:Addgene_19993)
  • For your References section:

    Identification of neurofibromin mutants that exhibit allele specificity or increased Ras affinity resulting in suppression of activated ras alleles. Morcos P, Thapar N, Tusneem N, Stacey D, Tamanoi F. Mol Cell Biol. 1996 May . 16(5):2496-503. PubMed 8628317