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pCAGGS-flpE-puro Citations (19)

Originally described in: Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells.
Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R Genesis. 2006 Jan . 44(1):23-8.
PubMed Journal

Articles Citing pCAGGS-flpE-puro

Inactivation of a novel FGF23 regulator, FAM20C, leads to hypophosphatemic rickets in mice. Wang X, Wang S, Li C, Gao T, Liu Y, Rangiani A, Sun Y, Hao J, George A, Lu Y, Groppe J, Yuan B, Feng JQ, Qin C. PLoS Genet. 2012;8(5):e1002708. doi: 10.1371/journal.pgen.1002708. Epub 2012 May 17. PubMed
Establishment of a rapid and scalable gene expression system in livestock by site-specific integration. Yu H, Wang X, Zhu L, He Z, Liu G, Xu X, Chen J, Cheng G. Gene. 2013 Feb 25;515(2):367-71. doi: 10.1016/j.gene.2012.10.017. Epub 2012 Oct 23. PubMed
Target-specific variants of Flp recombinase mediate genome engineering reactions in mammalian cells. Shah R, Li F, Voziyanova E, Voziyanov Y. FEBS J. 2015 Sep;282(17):3323-33. doi: 10.1111/febs.13345. Epub 2015 Jul 1. PubMed
Imprinted DNA methylation reconstituted at a non-imprinted locus. Taylor DH, McLean CM, Wu WL, Wang AB, Soloway PD. Epigenetics Chromatin. 2016 Sep 22;9:41. doi: 10.1186/s13072-016-0094-0. eCollection 2016. PubMed
CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance. Feng X, Hsu SJ, Kasbek C, Chaiken M, Price CM. Nucleic Acids Res. 2017 May 5;45(8):4281-4293. doi: 10.1093/nar/gkx125. PubMed
Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange. Pieters T, Haenebalcke L, Bruneel K, Vandamme N, Hochepied T, van Hengel J, Wirth D, Berx G, Haigh JJ, van Roy F, Goossens S. J Vis Exp. 2017 Apr 27;(122). doi: 10.3791/55575. PubMed
The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells. Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger J, Stadler MB, Giorgetti L, Chao JA. Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19. PubMed

Associated Plasmids

Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease. Phan QV, Contzen J, Seemann P, Gossen M. Sci Rep. 2017 Dec 19;7(1):17771. doi: 10.1038/s41598-017-17651-0. PubMed
Three classes of response elements for human PRC2 and MLL1/2-Trithorax complexes. Du J, Kirk B, Zeng J, Ma J, Wang Q. Nucleic Acids Res. 2018 Sep 28;46(17):8848-8864. doi: 10.1093/nar/gky595. PubMed
Genetic Liver-Specific AMPK Activation Protects against Diet-Induced Obesity and NAFLD. Garcia D, Hellberg K, Chaix A, Wallace M, Herzig S, Badur MG, Lin T, Shokhirev MN, Pinto AFM, Ross DS, Saghatelian A, Panda S, Dow LE, Metallo CM, Shaw RJ. Cell Rep. 2019 Jan 2;26(1):192-208.e6. doi: 10.1016/j.celrep.2018.12.036. PubMed
Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing. Huo Z, Tu J, Xu A, Li Y, Wang D, Liu M, Zhou R, Zhu D, Lin Y, Gingold JA, Yen CJ, Xiao H, Zhao R. Stem Cell Res. 2019 Jan;34:101360. doi: 10.1016/j.scr.2018.101360. Epub 2018 Nov 30. PubMed
Increased PHGDH expression promotes aberrant melanin accumulation. Mattaini KR, Sullivan MR, Lau AN, Fiske BP, Bronson RT, Vander Heiden MG. BMC Cancer. 2019 Jul 22;19(1):723. doi: 10.1186/s12885-019-5933-5. PubMed
Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9. Huo Z, Tu J, Lee DF, Zhao R. Methods Mol Biol. 2020;2108:355-369. doi: 10.1007/978-1-0716-0247-8_29. PubMed
KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells. Hao J, Yang X, Zhang C, Zhang XT, Shi M, Wang SH, Mi L, Zhao YT, Cao H, Wang Y. Cell Prolif. 2020 Nov;53(11):e12914. doi: 10.1111/cpr.12914. Epub 2020 Sep 29. PubMed
HOXA13 in etiology and oncogenic potential of Barrett's esophagus. Janmaat VT, Nesteruk K, Spaander MCW, Verhaar AP, Yu B, Silva RA, Phillips WA, Magierowski M, van de Winkel A, Stadler HS, Sandoval-Guzman T, van der Laan LJW, Kuipers EJ, Smits R, Bruno MJ, Fuhler GM, Clemons NJ, Peppelenbosch MP. Nat Commun. 2021 Jun 7;12(1):3354. doi: 10.1038/s41467-021-23641-8. PubMed
Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9. Anzai T, Hara H, Chanthra N, Sadahiro T, Ieda M, Hanazono Y, Uosaki H. Methods Mol Biol. 2021;2320:247-259. doi: 10.1007/978-1-0716-1484-6_22. PubMed
A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals. Holoch D, Wassef M, Lovkvist C, Zielinski D, Aflaki S, Lombard B, Hery T, Loew D, Howard M, Margueron R. Nat Genet. 2021 Dec;53(12):1686-1697. doi: 10.1038/s41588-021-00964-2. Epub 2021 Nov 15. PubMed
Single-molecule imaging reveals translation-dependent destabilization of mRNAs. Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA. Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1. PubMed

Associated Plasmids

Cell autonomous role of leucine-rich repeat kinase in protection of dopaminergic neuron survival. Kang J, Huang G, Ma L, Tong Y, Shahapal A, Chen P, Shen J. bioRxiv [Preprint]. 2024 Mar 7:2023.10.06.561293. doi: 10.1101/2023.10.06.561293. PubMed

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