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pET21-Streptavidin-Dead Aspartate Loop
(Plasmid #46368)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 46368 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 5800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Dh5alpha for propagation of the plasmid. Bl21[DE3] or similar required for protein expression.
  • Copy number
    Low Copy


  • Gene/Insert name
    Streptavidin-Dead Aspartate Loop
  • Alt name
  • Species
    E. coli
  • Insert Size (bp)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7F
  • 3′ sequencing primer T7R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21-Streptavidin-Dead Aspartate Loop was a gift from Mark Howarth (Addgene plasmid # 46368 ; ; RRID:Addgene_46368)
  • For your References section:

    Plug-And-Play Pairing Via Defined Divalent Streptavidins. Fairhead M, Krndija D, Lowe ED, Howarth M. J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016. 10.1016/j.jmb.2013.09.016 PubMed 24056174