pCDH-puro-cMyc
(Plasmid #46970)

• Purpose: lentiviral expression of c-Myc
• Depositing Lab: Jialiang Wang
• Publication: Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12.

Backbone

• Vector backbone: PCDH-CMV-MCS-EF1-PURO
• Backbone manufacturer: System Biosciences
• Total vector size (bp): 7384
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MYC
• Alt name: v-myc avian myelocytomatosis viral oncogene homolog
• Alt name: c-MYC
• Alt name: MRTL
• Species: H. sapiens (human)
• Insert Size (bp): 1323
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 46 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry