pAAVS1-NDi-CRISPRi (Gen1) Citations (22)
Originally described in: CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10. PubMed Journal
Articles Citing pAAVS1-NDi-CRISPRi (Gen1)
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Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development.
Genga RMJ, Kernfeld EM, Parsi KM, Parsons TJ, Ziller MJ, Maehr R.
Cell Rep. 2019 Apr 16;27(3):708-718.e10. doi: 10.1016/j.celrep.2019.03.076.
PubMed
Associated Plasmids |
KLF4 protein stability regulated by interaction with pluripotency transcription factors overrides transcriptional control.
Dhaliwal NK, Abatti LE, Mitchell JA.
Genes Dev. 2019 Jun 20. pii: gad.324319.119. doi: 10.1101/gad.324319.119.
PubMed
Associated Plasmids |
| Molecular mechanism for the multiple sclerosis risk variant rs17594362. Kim D, Park Y. Hum Mol Genet. 2019 Nov 1;28(21):3600-3609. doi: 10.1093/hmg/ddz216. PubMed |
| FGF primes angioblast formation by inducing ETV2 and LMO2 via FGFR1/BRAF/MEK/ERK. Chen PC, Hsueh YW, Lee YH, Tsai HW, Tsai KJ, Chiang PM. Cell Mol Life Sci. 2021 Mar;78(5):2199-2212. doi: 10.1007/s00018-020-03630-8. Epub 2020 Sep 10. PubMed |
| KRAS(G12C) Can Either Promote or Impair Cap-Dependent Translation in Two Different Lung Adenocarcinoma Cell Lines. Kyriakopoulos G, Katopodi V, Skeparnias I, Kaliatsi EG, Grafanaki K, Stathopoulos C. Int J Mol Sci. 2021 Feb 23;22(4). pii: ijms22042222. doi: 10.3390/ijms22042222. PubMed |
| Identifying oligodendrocyte enhancers governing Plp1 expression. Kim D, An H, Fan C, Park Y. Hum Mol Genet. 2021 Jul 6. pii: 6316230. doi: 10.1093/hmg/ddab184. PubMed |
| Generation of AAVS1 integrated doxycycline-inducible CRISPR-Prime Editor human induced pluripotent stem cell line. Bharucha N, Ataam JA, Gavidia AA, Karakikes I. Stem Cell Res. 2021 Nov 24;57:102610. doi: 10.1016/j.scr.2021.102610. PubMed |
| Comprehensive analysis of prime editing outcomes in human embryonic stem cells. Habib O, Habib G, Hwang GH, Bae S. Nucleic Acids Res. 2022 Jan 25;50(2):1187-1197. doi: 10.1093/nar/gkab1295. PubMed |
| Isogenic human SNCA gene dosage induced pluripotent stem cells to model Parkinson's disease. Zafar F, Nallur Srinivasaraghavan V, Yang Chen M, Alejandra Morato Torres C, Schule B. Stem Cell Res. 2022 Apr;60:102733. doi: 10.1016/j.scr.2022.102733. Epub 2022 Mar 1. PubMed |
| Generation of a dual edited human induced pluripotent stem cell Myl7-GFP reporter line with inducible CRISPRi/dCas9. Metzl-Raz E, Bharucha N, Arthur Ataam J, Gavidia AA, Greenleaf WJ, Karakikes I. Stem Cell Res. 2022 May;61:102754. doi: 10.1016/j.scr.2022.102754. Epub 2022 Mar 18. PubMed |
| Active endogenous retroviral elements in human pluripotent stem cells play a role in regulating host gene expression. Zhang T, Zheng R, Li M, Yan C, Lan X, Tong B, Lu P, Jiang W. Nucleic Acids Res. 2022 May 20;50(9):4959-4973. doi: 10.1093/nar/gkac265. PubMed |
| Identifying an oligodendrocyte enhancer that regulates Olig2 expression. Fan C, Kim D, An H, Park Y. Hum Mol Genet. 2023 Feb 19;32(5):835-846. doi: 10.1093/hmg/ddac249. PubMed |
| CRISPRi Gene Modulation and All-Optical Electrophysiology in Post-Differentiated Human iPSC-Cardiomyocytes. Han JL, Heinson YW, Chua CJ, Liu W, Entcheva E. bioRxiv. 2023 May 9:2023.05.07.539756. doi: 10.1101/2023.05.07.539756. Preprint. PubMed |
| Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo. Riemenschneider H, Simonetti F, Sheth U, Katona E, Roth S, Hutten S, Farny D, Michaelsen M, Nuscher B, Schmidt MK, Flatley A, Schepers A, Gruijs da Silva LA, Zhou Q, Klopstock T, Liesz A, Arzberger T, Herms J, Feederle R, Gendron TF, Dormann D, Edbauer D. Acta Neuropathol Commun. 2023 Jul 11;11(1):112. doi: 10.1186/s40478-023-01592-z. PubMed |
| CRISPRi gene modulation and all-optical electrophysiology in post-differentiated human iPSC-cardiomyocytes. Han JL, Heinson YW, Chua CJ, Liu W, Entcheva E. Commun Biol. 2023 Dec 7;6(1):1236. doi: 10.1038/s42003-023-05627-y. PubMed |
| OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology. Heinson YW, Han JL, Entcheva E. J Mol Cell Cardiol Plus. 2023 Dec;6:100054. doi: 10.1016/j.jmccpl.2023.100054. Epub 2023 Nov 9. PubMed |
Pioneer and PRDM transcription factors coordinate bivalent epigenetic states to safeguard cell fate.
Matsui S, Granitto M, Buckley M, Ludwig K, Koigi S, Shiley J, Zacharias WJ, Mayhew CN, Lim HW, Iwafuchi M.
Mol Cell. 2024 Jan 4:S1097-2765(23)01026-2. doi: 10.1016/j.molcel.2023.12.007.
PubMed
Associated Plasmids |
Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi.
Edenhofer FC, Termeg A, Ohnuki M, Jocher J, Kliesmete Z, Briem E, Hellmann I, Enard W.
iScience. 2024 May 23;27(6):110090. doi: 10.1016/j.isci.2024.110090. eCollection 2024 Jun 21.
PubMed
Associated Plasmids |
| In vivo single-cell CRISPR uncovers distinct TNF programmes in tumour evolution. Renz PF, Ghoshdastider U, Baghai Sain S, Valdivia-Francia F, Khandekar A, Ormiston M, Bernasconi M, Dure C, Kretz JA, Lee M, Hyams K, Forny M, Pohly M, Ficht X, Ellis SJ, Moor AE, Sendoel A. Nature. 2024 Aug;632(8024):419-428. doi: 10.1038/s41586-024-07663-y. Epub 2024 Jul 17. PubMed |
| Protocol for establishing inducible CRISPR interference system for multiple-gene silencing in human pluripotent stem cells. Matsui S, Shiley JR, Granitto M, Ludwig K, Buckley M, Koigi S, Mirizio G, Hu YC, Mayhew CN, Iwafuchi M. STAR Protoc. 2024 Sep 20;5(3):103221. doi: 10.1016/j.xpro.2024.103221. Epub 2024 Jul 31. PubMed |
| Protocol for establishing inducible CRISPRd system for blocking transcription factor-binding sites in human pluripotent stem cells. Matsui S, Shiley JR, Buckley M, Lim HW, Hu YC, Mayhew CN, Iwafuchi M. STAR Protoc. 2024 Sep 20;5(3):103233. doi: 10.1016/j.xpro.2024.103233. Epub 2024 Aug 11. PubMed |
| Prostatic lineage differentiation from human embryonic stem cells through inducible expression of NKX3-1. Wang S, Yu Y, Li Y, Zhang T, Jiang W, Wang X, Liu R. Stem Cell Res Ther. 2024 Sep 2;15(1):274. doi: 10.1186/s13287-024-03886-y. PubMed |
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