ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes.
Murphy KC, Nelson SJ, Nambi S, Papavinasasundaram K, Baer CE, Sassetti CM
MBio. 2018 Dec 11;9(6). pii: mBio.01467-18. doi: 10.1128/mBio.01467-18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108319 | pKM444 | For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase |
| 108320 | pKM461 | For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB |
| 108321 | pKM446 (Flag-DAS+4)) | Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker. |
| 108322 | pKM464 (deletions) | Orbit integrating plasmid to delete target gene. |
| 108434 | pKM468-EGFP | ORBIT integrating plasmid for C-terminal tagging with EGFP. |
| 108437 | pKM489-Snap | ORBIT integrating plasmid for C-terminal tagging with SNAP tag. |
| 109281 | pKM490-Clip | ORBIT integrating plasmid for C-terminal tagging with CLIP tag |
| 109282 | pKM491-Flag-His | ORBIT integrating plasmid for C-terminal tagging with FLAG-His tag |
| 109283 | pKM492-Myc-His | ORBIT integrating plasmid for C-terminal tagging with Myc-His tag |
| 109284 | pKM495 (Flag-Das+4) Zeo | ORBIT integrating plasmid for C-terminal tagging with degradation tag DAS+4, using zeocin as a selection marker. |
| 109301 | pKM496 (deletions) Zeo | ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker. |
| 140191 | pKM493 | ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP. |
| 140192 | pKM512 | For curing of Orbit-integrated plasmids and associated drug marker |