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Fungal Toolkit for Modular Cloning (FTK)
(Kit # 1000000191 )

Depositing Lab:   Arnold Driessen

The Fungal Toolkit for Modular Cloning consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native fungal promoters, terminators, selection markers, various CDSs for transcriptional activation and DNA-binding domains, fluorescent reporters, and the AMA1 sequence for fungal autonomous replication as well as CRISPR components such as Cas9, dCas9 sequences, and single guide RNA (sgRNA) transcription units for filamentous fungi.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$ 400 USD + shipping

Available to academics and nonprofits only.

Original Publications

Modular Synthetic Biology Toolkit for Filamentous Fungi Mózsik L, Pohl C, Meyer V, Bovenberg RAL, Nygård Y, Driessen AJM ACS Synthetic Biology. 2021 Nov 19; doi: 10.1021/acssynbio.1c00260. PubMed PMID: 34726388 .

CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mózsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygård Y, Driessen AJM Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. PubMed PMID: 34726388 .

Description

The Fungal Toolkit for Modular Cloning consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native fungal promoters, terminators, selection markers, various CDSs for transcriptional activation and DNA-binding domains, fluorescent reporters, and the AMA1 sequence for fungal autonomous replication as well as CRISPR components such as Cas9, dCas9 sequences, and single guide RNA (sgRNA) transcription units for filamentous fungi.

List of vectors in the Fungal Toolkit for Modular Cloning. Also shows transcriptional units and additional units, promoters, coding sequences, and terminators.
  • List of vectors in the Fungal Toolkit for Modular Cloning (FTK).

  • (a) Location of genetic parts in a transcription unit with their corresponding linker sequences.

  • (b) List of parts of the toolkit, containing promoters (P1), UASs (P2), UAS-compatible core promoters (P3), coding sequences with various fusion possibilities (CDS1–5), terminators (T1), complete transcription units (TUs), and additional vectors (sgRNA transcription units, flanking sequences, and AMA1 vectors).

  • Abbreviations (Pc, An, Ania, Ao, Sc) indicate the origin of the template (P. rubens, A. niger, A. nidulans, A. oryzae, S. cerevisiae, respectively).

  • (Image from Mózsik et al. ACS Synth Biol (2021) 10:11 https://doi.org/10.1021/acssynbio.1c00260, reprinted under the terms of the Creative Commons Attribution License.)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Fungal Toolkit for Modular Cloning (FTK) was a gift from Arnold Driessen (Addgene kit #1000000191).”

For your Reference section:

Modular Synthetic Biology Toolkit for Filamentous Fungi Mózsik L, Pohl C, Meyer V, Bovenberg RAL, Nygård Y, Driessen AJM ACS Synthetic Biology. 2021 Nov 19; doi: 10.1021/acssynbio.1c00260. PubMed PMID: 34726388 .

CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mózsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygård Y, Driessen AJM Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. PubMed PMID: 34726388 .

Fungal Toolkit for Modular Cloning (FTK) - #1000000191

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Spectinomycin
Ampicillin
Chloramphenicol

Inventory

Well Plasmid Resistance
A / 1 pFTK001
Spectinomycin
A / 2 pFTK002
Spectinomycin
A / 3 pFTK003
Spectinomycin
A / 4 pFTK004
Spectinomycin
A / 5 pFTK005
Spectinomycin
A / 6 pFTK006
Spectinomycin
A / 7 pFTK007
Spectinomycin
A / 8 pFTK008
Spectinomycin
A / 9 pFTK009
Spectinomycin
A / 10 pFTK010
Spectinomycin
A / 11 pFTK011
Spectinomycin
A / 12 pFTK012
Spectinomycin
B / 1 pFTK013
Spectinomycin
B / 2 pFTK014
Spectinomycin
B / 3 pFTK015
Spectinomycin
B / 4 pFTK016
Spectinomycin
B / 5 pFTK017
Spectinomycin
B / 6 pFTK018
Spectinomycin
B / 7 pFTK019
Spectinomycin
B / 8 pFTK020
Spectinomycin
B / 9 pFTK021
Spectinomycin
B / 10 pFTK022
Spectinomycin
B / 11 pFTK023
Spectinomycin
B / 12 pFTK024
Spectinomycin
C / 1 pFTK025
Spectinomycin
C / 2 pFTK026
Spectinomycin
C / 3 pFTK027
Spectinomycin
C / 4 pFTK028
Spectinomycin
C / 5 pFTK029
Spectinomycin
C / 6 pFTK030
Spectinomycin
C / 7 pFTK031
Spectinomycin
C / 8 pFTK032
Spectinomycin
C / 9 pFTK033
Spectinomycin
C / 10 pFTK034
Spectinomycin
C / 11 pFTK035
Spectinomycin
C / 12 pFTK036
Spectinomycin
D / 1 pFTK037
Spectinomycin
D / 2 pFTK038
Spectinomycin
D / 3 pFTK039
Spectinomycin
D / 4 pFTK040
Spectinomycin
D / 5 pFTK041
Spectinomycin
D / 6 pFTK042
Spectinomycin
D / 7 pFTK043
Spectinomycin
D / 8 pFTK044
Spectinomycin
D / 9 pFTK045
Spectinomycin
D / 10 pFTK046
Spectinomycin
D / 11 pFTK047
Spectinomycin
D / 12 pFTK048
Spectinomycin
E / 1 pFTK049
Spectinomycin
E / 2 pFTK050
Spectinomycin
E / 3 pFTK051
Spectinomycin
E / 4 pFTK052
Spectinomycin
E / 5 pFTK053
Spectinomycin
E / 6 pFTK054
Spectinomycin
E / 7 pFTK055
Spectinomycin
E / 8 pFTK056
Spectinomycin
E / 9 pFTK057
Spectinomycin
E / 10 pFTK058
Spectinomycin
E / 11 pFTK059
Spectinomycin
E / 12 pFTK060
Spectinomycin
F / 1 pFTK061
Spectinomycin
F / 2 pFTK062
Spectinomycin
F / 3 pFTK063
Spectinomycin
F / 4 pFTK064
Spectinomycin
F / 5 pFTK065
Spectinomycin
F / 6 pFTK066
Spectinomycin
F / 7 pFTK067
Spectinomycin
F / 8 pFTK068
Spectinomycin
F / 9 pFTK069
Spectinomycin
F / 10 pFTK070
Spectinomycin
F / 11 pFTK071
Spectinomycin
F / 12 pFTK072
Spectinomycin
G / 1 pFTK073
Spectinomycin
G / 2 pFTK074
Spectinomycin
G / 3 pFTK075
Spectinomycin
G / 4 pFTK076
Spectinomycin
G / 5 pFTK077
Spectinomycin
G / 6 pFTK078
Spectinomycin
G / 7 pFTK079
Spectinomycin
G / 8 pFTK080
Spectinomycin
G / 9 pFTK081
Spectinomycin
G / 10 pFTK082
Spectinomycin
G / 11 pFTK083
Spectinomycin
G / 12 pFTK084
Spectinomycin
H / 1 pFTK085
Spectinomycin
H / 2 pFTK086
Spectinomycin
H / 3 pFTK087
Spectinomycin
H / 4 pFTK088
Spectinomycin
H / 5 pFTK089
Spectinomycin
H / 6 pFTK090
Spectinomycin
H / 7 pFTK091
Ampicillin
H / 8 pFTK092
Ampicillin
H / 9 pFTK093
Ampicillin
H / 10 pFTK094-LM-AMA002.0
Chloramphenicol
H / 11 pLM-AMA15.0 Cas9
Chloramphenicol
H / 12 pLM-AMA18.0 dCas9-VPR
Chloramphenicol
Data calculated @ 2024-02-22

Kit Plate Map - #1000000191

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