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This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota, such as Escherichia coli, Pseudomonas putida, Cupriavidus necator, and Komagataeibacter nataicola.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota. The vectors contain resistance cassettes for kanamycin, chloramphenicol, and spectinomycin. A combinatorial library of constructs expressing GFP at 18 levels for rapid characterization of expression parts in new organisms is also included.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The openCIDAR MoClo Toolkit was a gift from Eric Young (Addgene kit #1000000227).”