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Human CRISPR Activation Pooled Library (Calabrese VP64)
(Pooled Libraries #1000000110, #92377, #92378)

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  • Purpose

    This human CRISPR activation library (Calabrese VP64) is in backbone pXPR_501 (VP64). This Calabrese library activates over 18,000 human genes and is used for genome-wide activation screening.

    Each gene activated by this library is targeted by 3-6 gRNAs. These gRNAs are split among the two pools: Set A (Cat# 92377) contains gRNAs 1–3, Set B (Cat# 92378) contains gRNAs 4–6. Each pool has a unique set of gRNAs, but both pools are designed to target the same genes. The slight discrepancy in number of genes targeted between Set A and Set B is because not all genes had 6 gRNAs.

  • Vector Backbone

    pXPR_501 (Plasmid #96922), which uses VP64 to activate a gRNA-scaffold.

    Note: This library will also be available in backbone pXPR_502 (Plasmid #96923), which uses P65-HSF to activate a gRNA-scaffold.

    P65-HSF (backbone pXPR_502) is a stronger transcriptional activator than VP64 (backbone pXPR_501).

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000110
  • Two tubes, each with a half-library (Set A and B) in backbone pXPR_501 (VP64)†
    • #92377 (Set A)
    • #92378 (Set B)
 1 Pending
Pooled Library 92377 Set A, one tube, contains Human CRISPRa sgRNA library Calabrese (VP64) 1
Pooled Library 92378 Set B, one tube, contains Human CRISPRa sgRNA library Calabrese (VP64) 1
Available to academic and nonprofits only.
† A dCas9 activation plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lenti dCAS-VP64_Blast (Addgene #61425).

Library Details

  • Species
    Human
  • Genes targeted
    18,885 (Set A); 18,843 (Set B)
  • gRNAs
    56,762 (Set A); 56,476 (Set B)
  • Controls
    500 unique non-targeting controls are in each of the two subpools (Set A and Set B)
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

Please visit https://doi.org/10.1101/356626 (Link opens in a new window) for bioRxiv preprint.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Calabrese CRISPR Activation Pooled Library (Set A and B) was a gift from David Root and John Doench (Addgene #1000000110)
    Human Calabrese CRISPR Activation Pooled Library Set A was a gift from David Root and John Doench (Addgene #92377 ; http://n2t.net/addgene:92377 ; RRID:Addgene_92377)
    Human Calabrese CRISPR Activation Pooled Library Set B was a gift from David Root and John Doench (Addgene #92378 ; http://n2t.net/addgene:92378 ; RRID:Addgene_92378)

  • For your References section:

    Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities. Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG. Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8. PubMed 30575746
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