-
-
PL452-EN1-LA-p2a-mNeonGreen-hGHpA-PGK-neoR-EN1-RA
Plasmid#191684PurposeFor knocking p2a-mNeonGreen into EN1 locusDepositorInsertEN1 homology arms and p2a-mNeonGreen
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA
Plasmid#191691PurposeFor knocking p2a-mTagBFP2nls-3xHAtag into EN1 locusDepositorInsertEN1 homology arms and p2a-mTagBFP2nls-3xHAtag
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pChillyBoys_gen1
Plasmid#228495PurposepACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)DepositorInsertsgroEL
groES
UseTagsExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
ZsGreen1-N1
Plasmid#54702PurposeLocalization: N1 Cloning Vector, Excitation: 493, Emission: 505DepositorTypeEmpty backboneUseTagsZsGreen1ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN12A-P1
Plasmid#27366DepositorInsertPmyc1tetO
UseGateway donor vectorTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP
Plasmid#200910Purpose3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expressionDepositorInsert3XHA_den1FnCas9_KRAB_eGFP
UseCRISPRTags3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFPExpressionMammalianMutationChanged Glutamic Acid to Arginine at 1369 positionPromoterCBHAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pDONR223_GEN1_WT
Plasmid#81844PurposeGateway Donor vector containing GEN1 , part of the Target Accelerator Plasmid Collection.DepositorInsertGEN1 (GEN1 Human)
UseGateway entry vectorTagsExpressionMutationS92TPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN12RRFuzzy3MYB
Plasmid#194441PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYBDepositorInsertgRNA array targeting CEN1, RR, Fuzzy3MYB
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
-
-
pORE303N_CEN12Fuzzy3MYB
Plasmid#194440PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYBDepositorInsertgRNA array targeting CEN1, Fuzzy3MYB
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN1
Plasmid#194439PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1DepositorInsertgRNA targeting CEN1
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_GEN1_p.L235R
Plasmid#81541PurposeGateway Donor vector containing GEN1 , part of the Target Accelerator Plasmid Collection.DepositorInsertGEN1 (GEN1 Human)
UseGateway entry vectorTagsExpressionMutationS92T; L235RPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FEN1
Plasmid#215129PurposeExpresses mCherry-FEN1 in mammalian cells from a retroviral vector.DepositorInsertFEN1 (FEN1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP
Plasmid#229227PurposeModular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.DepositorTypeEmpty backboneUseTagsmNeonGreen11ExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1hWT
Plasmid#35027DepositorInsertFEN1 (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1DA
Plasmid#35028DepositorInsertFEN1DA (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationD181A--NUCLEASE DEAD POINT MUTANTPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1mWT
Plasmid#35032DepositorInsertFEN1mWT
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1deltaP
Plasmid#35030DepositorInsertFEN1DELTAP (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutation8 AMINO ACID DELETION OF P BOX (amino acids 337–3…PromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-TALEN1
Plasmid#83368PurposePartner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectorsDepositorInsertGAPDH_TALEN1
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ZnGreen1
Plasmid#169470Purposecytosolic ZnGreen1 zinc sensor with 633 nM KdDepositorInsertZnGreen1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1082_Rad21_STOP
Plasmid#156450PurposeFor transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codonDepositorInsertCas9, RAD21 (Rad21 Mouse)
UseCRISPRTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEN1-SGC
Plasmid#39127PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsStrep tag and SET1ExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZsGreen1-H2B-6
Plasmid#56514PurposeLocalization: Nucleus/Histones, Excitation: 493, Emission: 505DepositorInsertH2B (H2BC11 Human)
UseTagsZsGreen1ExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only
