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Showing: 1 - 30 of 31294 results

  1. GreenGate

    Plasmid Kit
    #1000000036
    Purpose
    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette
    Depositor
    Jan Lohmann
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. GreenGate 2.0 Toolkit

    Plasmid Kit
    #1000000241
    Purpose
    The GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.
    Depositor
    Alexis Maizel
    Available since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  3. pGoldenGreenGate-M (pGGG-M)

    Plasmid
    #165422
    Purpose
    A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology; Level 2 golden gate (moclo) du…
    Tags
    Expression
    Mutation
    Promoter
    Contain GG cassette and MCS with LacZ for blue/wh…
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165422-plasmid-map-sequence-id-331272

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  4. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit
    #1000000109
    Purpose
    Build sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.
    Depositor
    Bob Goldstein
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.
    Depositor
    Daniel Voytas
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  10. pVE2_20_p40S_QfDBD_VP16AD_pFullLength_pcbAB_DsRed

    Plasmid
    #154242
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbAB
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Mutation
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154242-plasmid-map-sequence-id-305991

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  11. pVE2_19_p40S_QfDBD_VP16AD_pFullLength_pcbC_DsRed

    Plasmid
    #154241
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbC
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Mutation
    Promoter
    p40S (AN0465 A. nidulans)
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154241-plasmid-map-sequence-id-306043

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  19. ZP11 Eng2

    Plasmid
    #16930
    Depositor
    Randall Moon
    Insert
    eng2 (eng2a Zebrafish)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only

  23. pcDNA3-EngPD

    Plasmid
    #64039
    Purpose
    Mammalian expression of paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2
    Insert
    Pax8 paired domain (Pax8 Mouse)
    Use
    Tags
    Eng (N-terminal repressor domain of the drosophi…
    Expression
    Mammalian
    Mutation
    paired domain, aa 1-147
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pT22Ubm4Eng2bKRPC(#493)

    Plasmid
    #184091
    Purpose
    cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:
    Insert
    4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    4xMyc
    Expression
    Mutation
    ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184091-plasmid-map-sequence-id-363977

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Showing: 1 - 30 of 31294 results