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Showing: 1 - 40 of 83 results

  1. Flag-M4-AR-K313A-K318A

    Plasmid
    #171260
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys313Ala-Lys318Ala (AR-K313A-K318A)
    Depositor
    Steven Balk
    Insert
    3xFlag-tagged human androgen receptor Lys313Ala-Lys318Ala mutant (AR Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Lys313Ala-Lys318Ala (AR-K313A-K318A)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171260-plasmid-map-sequence-id-336033

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  6. pTK313

    Plasmid
    #59570
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59570-plasmid-map-sequence-id-92975

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  7. pTK317

    Plasmid
    #59571
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59571-plasmid-map-sequence-id-92976

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  8. pTK312

    Plasmid
    #59569
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP XbaI attB1 Kozak EGFP ochre attB2 AscI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59569-plasmid-map-sequence-id-92974

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  9. pCK314

    Plasmid
    #110545
    Purpose
    Modified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021
    Inserts
    Modified PrhaBAD (CRP-binding sites removed)
    yfp
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110545-plasmid-map-sequence-id-212174

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  13. pKEK3194

    Plasmid
    #234094
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Insert
    DasherGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234094-plasmid-map-sequence-id-464934

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  14. pKEK3197

    Plasmid
    #234095
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Insert
    YukonOFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234095-plasmid-map-sequence-id-464928

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  15. pKEK3198

    Plasmid
    #234096
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Insert
    aeBlue
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234096-plasmid-map-sequence-id-464932

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  16. pKEK3199

    Plasmid
    #234097
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Insert
    tspurple
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234097-plasmid-map-sequence-id-464933

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  17. pKEK3193

    Plasmid
    #234093
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Insert
    eforCP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234093-plasmid-map-sequence-id-464931

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  19. pJK317

    Plasmid
    #72472
    Purpose
    puc118 modified to contain ribosome binding site
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72472-plasmid-map-sequence-id-140449

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  20. pJK319

    Plasmid
    #71143
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to alanine; additional N-ter…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71143-plasmid-map-sequence-id-131042

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  21. pJK318

    Plasmid
    #71142
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to glutamine; additional N-t…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71142-plasmid-map-sequence-id-131041

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  22. pJK315

    Plasmid
    #71141
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to glycine; additional N-ter…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71141-plasmid-map-sequence-id-131040

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  23. pJK314

    Plasmid
    #71140
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to valine; additional N-term…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71140-plasmid-map-sequence-id-131039

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  29. pcDNA3.1_ZKSCAN1_Tau10-12K317M

    Plasmid
    #194164
    Purpose
    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 WT (MAPT Human)
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Lysine 317 to Methionine
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194164-plasmid-map-sequence-id-381805

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  30. Flag-M4-AR-K318A

    Plasmid
    #171259
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor Lysine mutant: AR-Lys318Ala (AR-K318A)
    Depositor
    Steven Balk
    Insert
    3xFlag-tagged human androgen receptor Lys318Ala mutant (AR Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Lys318Ala (AR-K318A)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171259-plasmid-map-sequence-id-336034

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  39. pcDNA3.1_Tau Authentic Alu 10-12K317M

    Plasmid
    #194168
    Purpose
    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 K317M
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Lysine 317 to Methionine
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194168-plasmid-map-sequence-id-381799

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  40. p663-UBC-miniTurbo-V5-STK31_IDG-K

    Plasmid
    #189880
    Purpose
    Gateway destination clone of STK31 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    STK31 (STK31 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189880-plasmid-map-sequence-id-374486

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Showing: 1 - 40 of 83 results