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pAAV-Syn-SomaGCaMP7

Plasmid

#158759

Purpose

Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Plasmid

#158967

Purpose

Ubiquitous expression of mtON in C. elegans

Depositor

Andrew Wojtovich

Article

Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

Insert

eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Tags

GFP

Expression

Worm

Promoter

eft-3

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2T

Plasmid

#15808

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2T (UBE2T Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDESTpK7WG2mito

Plasmid

#158726

Purpose

Plant mitoTALEN vector assembly, Destination vector

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

mitochondrial presequence in front of attR1 in pk7WG2

Expression

Plant

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMT_025

Plasmid

#158579

Purpose

Lentiviral expression of a customizable open reading frame

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.AU1.V5_NGFR

Plasmid

#158235

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HA.AU1.V5

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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scFLARE1-p2A-eGFP

Plasmid

#158699

Purpose

Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE1-p2A-eGFP

Use

AAV

Tags

Flag, V5. eGFP

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR8A L3-L2

Plasmid

#158735

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF8A L1-L4

Plasmid

#158731

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NN)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF5A L1-L4

Plasmid

#158728

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last HD)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Rho*-BERKY3

Plasmid

#158220

Purpose

BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLIX403-ccdB-NatMx

Plasmid

#158563

Purpose

Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR6A L3-L2

Plasmid

#158733

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NG)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR7A L3-L2

Plasmid

#158734

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NI)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF6A L1-L4

Plasmid

#158729

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NG)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS

Plasmid

#158739

Purpose

Expresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Hemagglutinin A/TX/50/12 H3

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

G186V S219F

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ND4 right TALE–G1397 DddAtox C

Plasmid

#158097

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ND4 left TALE–G1397 DddAtox N

Plasmid

#158094

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 WC visual barcode

Plasmid

#158666

Purpose

tagging clonal populations with whole cell mTAGBFP2 fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pKL10-mRuby-iATPSnFR1.0 bb118

Plasmid

#158980

Purpose

Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

mruby-iATPSNfR1.0

Tags

mruby3

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#102557

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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