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Showing: 181 - 200 of 252 results

  3. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid
    #216377
    Purpose
    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)
    Depositor
    William Weis
    Insert
    LDL receptor related protein 6 (LRP6 Human)
    Use
    Baculovirus
    Tags
    9xHis and HA signal sequence-FLAG-3C cleavage sit…
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin (LRP6)/p10 (MESD)
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216377-plasmid-map-sequence-id-433174

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  6. pJBL729

    Plasmid
    #140399
    Purpose
    Expresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-cadO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140399-plasmid-map-sequence-id-297856

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  7. p667-UBC-COQ8B-V5-miniTurbo_IDG-K

    Plasmid
    #189915
    Purpose
    Gateway destination clone of COQ8B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    COQ8B (ADCK4 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    F435S
    Promoter
    Ubiquitin
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    189915-plasmid-map-sequence-id-374112

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  10. p667-UBC-COQ8A-V5-miniTurbo_IDG-K

    Plasmid
    #189885
    Purpose
    Gateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    COQ8A (COQ8A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189885-plasmid-map-sequence-id-374487

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  12. LRP6-m5-pCS2-VSVG

    Plasmid
    #27283
    Depositor
    Xi He
    Article
    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.
    Insert
    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    Use
    Xenopus injection
    Tags
    VSVG
    Expression
    Mutation
    Changed S/T of all five PPP(S/T)P motifs to Alani…
    Promoter
    Available since
    March 3, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70703-plasmid-map-sequence-id-127765

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  15. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  16. pJK193

    Plasmid
    #71834
    Purpose
    Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Tags
    Expression
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71834-plasmid-map-sequence-id-131053

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  19. LRP6 dC-pCS2

    Plasmid
    #27258
    Depositor
    Xi He
    Article
    Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.
    Insert
    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    Use
    Xenopus injection
    Tags
    Expression
    Mammalian
    Mutation
    carboxyl intracellular region deleted Residue 14…
    Promoter
    Available since
    Feb. 10, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 181 - 200 of 252 results