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Plasmid#167169PurposeExpresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.DepositorArticleInsertAPOBEC1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E4-T2A-Puro
Plasmid#190813PurposeExpress APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE4PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E3-T2A-Puro
Plasmid#190812PurposeExpress APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE3PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E2-T2A-Puro
Plasmid#190811PurposeExpress APOE E2 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE2PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-TAL-apobb.1-PR2-RA
Plasmid#128696PurposeRight arm of TALEN pair 2 for cleavage of apobb.1 STOP locusDepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-TAL-apobb.1-PR2-LA
Plasmid#128695PurposeLeft arm of TALEN pair 2 for cleavage of apobb.1 STOP locusDepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP
Plasmid#89062PurposeAAV-gRNA targeting the murine Apob geneDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-SEC61-FAPoptim
Plasmid#221126PurposeFAP-tagged marker for the ER (FAP optimized for yeast expression)DepositorInsertSEC61-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC63-FAPoptim
Plasmid#221116PurposeFAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertFAPoptim-SEC63
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC61-FAPoptim
Plasmid#221115PurposeFAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertSEC61-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-MFA-NFAPoptim
Plasmid#221095PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-ANP1-FAPoptim
Plasmid#221120PurposeFAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)DepositorInsertANP1-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-ERG6-FAPoptim
Plasmid#221121PurposeFAP-tagged marker for lipid droplets (FAP optimized for yeast expression)DepositorInsertERG6-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-PMA1-FAPoptim
Plasmid#221122PurposeFAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)DepositorInsertPMA1-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-RPA34-FAPoptim
Plasmid#221123PurposeFAP-tagged marker for the nucleus (FAP optimized for yeast expression)DepositorInsertRPA34-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-SEC7-FAPoptim
Plasmid#221124PurposeFAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)DepositorInsertSEC7-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-VPH1-FAPoptim
Plasmid#221125PurposeFAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)DepositorInsertVPH1-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-MFA-NFAPoptim
Plasmid#221090PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-EA-evoAPOBEC1max_NG-BlastR
Plasmid#232721PurposeC-to-T base editorDepositorArticleInsertevoAPOBEC1max H47E+S48A-nCas9(NG)-UGI-UGI
UseTagsExpressionMammalianMutationwithin evoAPOBEC1: H47E+S48A; within Cas9 D10APromoterAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-APOBEC-nCas9-Ung
Plasmid#198539PurposeExpresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-APOBEC-nCas9-Ung
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only