We narrowed to 27,413 results for: CAT;

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3 gRNA concatemer

Plasmid

#84880

Purpose

Empty concatmer vector in which 3 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only
LLP784_SnoopCatcher-DNMT3A-CO

Plasmid

#211777

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Spy-EZH2

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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InterCatch-1xL

Plasmid

#186907

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-(GGGGS)1-SpyCatcher003-sfCherry1-10

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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USPL1cat-pETM30

Plasmid

#85768

Purpose

bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514) (USPL1 Human)

Tags

GST and His

Expression

Bacterial

Mutation

catalytic domain (aa 212-514)

Promoter

T7-lac

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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LLP787_SnoopCatcher-EZH2-FL-CO

Plasmid

#211780

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP780_SpyCatcher-KRAB-CO

Plasmid

#211773

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-KRAB

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

Plasmid

#116963

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET26b(+)-ATI_QconCAT_1

Plasmid

#163957

Purpose

Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

Depositor

Stefan Tenzer

Article

Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

Insert

ATI QconCAT

Tags

polyhistidine tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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mPlum-CathespinB-6

Plasmid

#55955

Purpose

Localization: Lysosomes, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

CathespinB (Ctsb Rat)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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258/-2.5CAT

Plasmid

#25431

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter/Enhancer (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

Plasmid

#116965

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCAT.015

Plasmid

#119558

Purpose

Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-CathespinB-6

Plasmid

#55467

Purpose

Localization: Lysosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CathespinB

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
ACS2 Truncated

Plasmid

#13748

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Truncated Acetyl CoA Sythetase 2 (Acss1 Mouse)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 39–682

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
USPL1cat-pEYFP_C1

Plasmid

#85766

Purpose

mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514) (USPL1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

catalytic domain (aa 212-514)

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC

Plasmid

#116964

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Ecat1

Plasmid

#13351

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell associated transcript 1 (2410004A20Rik Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Tags

6x His tag and SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-Ecat8

Plasmid

#13357

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell associated transcript 8 (Tdrd12 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
mApple-CathespinB-6

Plasmid

#54871

Purpose

Localization: Lysosomes, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

CathespinB

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only