We narrowed to 2,394 results for: EFS

1. pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)

   Plasmid

   #192190

   Purpose

   HER2 CAR AAV vector PRODH2 KI (pLY080b)

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   HER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)
   

   Use

   AAV; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

   Plasmid

   #85452

   Purpose

   Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189925

   Purpose

   AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SauriABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SauriCas9 D15A

   Promoter

   EFS

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM123: pAAV-EFS-CasRx-control presgRNA

   Plasmid

   #166871

   Purpose

   AAV vector for expressing CasRx and control presgRNA for RNA-editing

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Inserts

   U6-control presgRNA
   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189923

   Purpose

   AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e V106W
   

   Use

   AAV

   Tags

   Expression

   Mutation

   nSaCas9 KKH, TadA ABE8e V106W

   Promoter

   EFS

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold

   Plasmid

   #226921

   Purpose

   Encoding SauriABE8e and sgRNA cassette on a single AAV

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   TadA8e, nSauriCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189922

   Purpose

   AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SaCas9 D10A

   Promoter

   EFS

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-Trono-EFS-Blast-P2A-TurboRFP

   Plasmid

   #228893

   Purpose

   Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid

    #99248

    Purpose

    single-vector system with EGFP reporter for use in CROPseq knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

    Plasmid

    #212962

    Purpose

    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_non-targeting spacer
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid

    #212963

    Purpose

    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_GFP targeting spacer
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189924

    Purpose

    AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e V106W
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SaCas9 D10A, TadA ABE8e V106W

    Promoter

    EFS

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223159

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid

    #52054

    Purpose

    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)

    Depositor

    Jesse Rinehart

    Inserts

    SepRS
    EF-Sep
    5x tRNA Sep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Promoter

    Ptac

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME EFS-rtTA/rtTA3 (JDW 1230)

    Plasmid

    #224525

    Purpose

    A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EFS-rtTA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

    Plasmid

    #223149

    Purpose

    Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDF0336 pAAV EFS-HvsCas7-11 for dual vector

    Plasmid

    #172515

    Purpose

    Encodes HvsCas7-11 mammalian expression casette in AAV backbone

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV EFS-HvsCas7-11 for dual vector
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLY101-RetroEFS-HER2-28BBz-PRODH2-WPRE

    Plasmid

    #192202

    Purpose

    Retro-HER2CAR-PRODH2

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Retro-HER2CAR-PRODH2 (ERBB2 Synthetic)
    

    Use

    Retroviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA

    Plasmid

    #113686

    Purpose

    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    KRAB

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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