Addgene

We narrowed to 2,484 results for: GCG

Showing: 181 - 200 of 2484 results

Results per page:

Sort results by:

pLKO-tet-puro-sh-ex16b

Plasmid

#35168

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pIS1-mutant RhoA 3'UTR

Plasmid

#26090

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mutant RhoA 3'UTR (RHOA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Both miR-31 binding sites in 3’ UTR mutagenized. …

Promoter

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only
MSCV-shCasp2-IRES-GFP

Plasmid

#52061

Purpose

stable knockdown of Caspase-2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Caspase-2 shRNA (CASP2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/T0-Flag-53BP1

Plasmid

#52507

Purpose

mammalian expression vector of 53BP1

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

V128A and siRNA resistant sequence: ACGCGCTGACGAC…

Promoter

CMV/TO

Available since

March 26, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR-53BP1 1-1972 28A

Plasmid

#53447

Purpose

Recombinational donor/master vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Gateway

Tags

Expression

Mutation

I914T mutation; All 28 SQ/TQ motifs in N-term of …

Promoter

Available since

Dec. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
px335 Mettl14 sgRNA #1

Plasmid

#61515

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gcggcagctcctagctcagc

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Use

Tags

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Promoter

Available since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
pAFMW-Nbr[K276A]

Plasmid

#188597

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

K276A [AAG=>GCG]

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr[K284A]

Plasmid

#188595

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

K284A [AAG=> GCG]

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr[R272A]

Plasmid

#188594

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG]

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

Plasmid

#52505

Purpose

mammalian expression vector of 53BP1 28A mutant

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

53BP1 28A (TP53BP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

All 28 SQ/TQ motifs in N-term of 53BP1 have been …

Promoter

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSG5-HA-ERR3 S57,81,219A

Plasmid

#37850

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

Estrogen-related receptor gamma (Esrrg Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Serines 57, 81, and 219 to Alanine (AGC t…

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
pAFMW-Nbr[R280A,K284A]

Plasmid

#188598

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

R280A [CGA=>GCA], K284A [AAG=> GCG]

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK01_Rho_minprox_DsREd

Plasmid

#173489

Purpose

PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

Depositor

Barak Cohen

Article

White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Insert

Mouse Rhodopsin promoter driving DsRed reporter gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Mouse Rhodopsin

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pgRNA-CGG

Plasmid

#108248

Purpose

Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

sgRNA targeting GGCGGCGGCGGCGGCGGCGG

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFlare9A-Clip2E9 Mutant

Plasmid

#209575

Purpose

minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Clip2 (Clip2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

Promoter

CMV

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-hHSF2 shRNA

Plasmid

#118346

Purpose

This plasmid expresses shRNA against hHSF2.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF2 shRNA (HSF2 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217342

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FgH1tUTG

Plasmid

#70183

Purpose

Inducible expression of guide RNA with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

H1-Tet-sgrna cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Pmyo-3::hpo-30 gDNA (pBAB204)

Plasmid

#117385

Purpose

Allows for body-wall muscle expression of HPO-30

Depositor

Kavita Babu

Article

Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

Insert

genomic DNA of hpo-30 (833 bp)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge