We narrowed to 15,002 results for: TIM;

1. pTF261 (pFA6a-TEV-6xGly-HphMX)

   Plasmid

   #44080

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTF262 (pFA6a-PSc-6xGly-HphMX)

   Plasmid

   #44074

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTF259 (pFA6a-TEV-6xGly-KanMX)

   Plasmid

   #44092

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. human EB1 fused to monomeric RFP

   Plasmid

   #39323

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Inserts

   EB1 (MAPRE1 Human)
   mRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

   Plasmid

   #39299

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Reduced Expression GFP beta actin

   Plasmid

   #31502

   Depositor

   Tim Mitchison, Rick Horwitz

   Article

   Watanabe et al Science. 2002 Feb 8. 295(5557):1083-6.

   Insert

   beta actin (ACTB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   91-544 of enhancer region of CMV promoter deleted

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. human EB1 in PET28a for protein expression

   Plasmid

   #39297

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Article

   Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

   Plasmid

   #39300

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S16A, R17A, H18A, D19A, W23A and N25A

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

9. human EB1 in pBluescript II

   Plasmid

   #39324

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

10. human EB1-GFP mutagenized to remove terminal 7 aa (QEEQEEY) (JB162))

    Plasmid

    #39308

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-terminal 7 aa (QEEQEEY) removed

    Promoter

    CMV

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

11. human EB1-GFP mutagenized Y44 Q46 both converted to A (JB140)

    Plasmid

    #39301

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y44A and Q46A

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

12. human EB1-GFP mutagenized D88 K89 D94 K95 all converted to A (JB141)

    Plasmid

    #39302

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D88A, K89A, D94A and K95A

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

13. human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)

    Plasmid

    #39303

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q109A, W110A, K112A and K113A

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

14. human EB1-GFP mutagenized K122 D123 Y124 D125 all converted to A (JB143)

    Plasmid

    #39304

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K122A, D123A, Y124A and D125A

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. human EB1-GFP mutagenized K148 K150 K151 all converted to A (JB144)

    Plasmid

    #39305

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K148A, K150A and K151A

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

16. human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)

    Plasmid

    #39306

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E211A, K212A, E213A, R214A, D215A and Y217A

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

17. human EB1-GFP mutagenized T249 D250 E251 all converted to A (JB146)

    Plasmid

    #39307

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T249A, D250A and E251A

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. Protein Purification Optimization Kit Using SUMO Protease Elution

    Plasmid Kit

    #1000000258

    Purpose

    A validation set of 96 unique, single-domain proteins that are structurally characterized, disulfide-free, mesophilic, and designed to be diverse in topology, size, and isoelectric points.

    Depositor

    Christopher Bahl

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

19. pMitoTimer

    Plasmid

    #52659

    Purpose

    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

    Depositor

    Zhen Yan

    Article

    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

    Insert

    DsRed1-E5
    

    Use

    Dsred

    Tags

    human cytochrome c oxidase subunit VIII mitochond…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiMPH v2

    Plasmid

    #89308

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Insert

    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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