We narrowed to 1,578 results for: nps

1. pCMV-EPN-01(ΔI)

   Plasmid

   #80048

   Purpose

   Expression of EPN-01 assembly mutant L33R

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN-01(L33R)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   L33R

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Puro 2.0

   Plasmid

   #24970

   Depositor

   Tyler Jacks

   Article

   Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIP-AMPKa1_KD

   Plasmid

   #79011

   Purpose

   For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47R

   Promoter

   pCAGGs

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-Ub

   Plasmid

   #11928

   Purpose

   Used for in vivo monitoring of ubiquitin localization.

   Depositor

   Nico Dantuma

   Article

   Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9-GFP_sg_mTfeb

   Plasmid

   #79006

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   sgTfeb (Tfeb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIP-Flag-TfebAA

   Plasmid

   #79014

   Purpose

   For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Tfeb (Tfeb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S142A, S211A

   Promoter

   pCAGGs

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas9-GFP_sg_mAMPKa1

   Plasmid

   #79004

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa1 (Prkaa1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCIP-caTfeb

   Plasmid

   #79013

   Purpose

   For mammalian expression of a shortened, constitutively active version of Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Tfeb (TFEB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Removed first ~400 bp from 5' end (N-terminu…

   Promoter

   pCAGGs

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pLVE-eGFP

   Plasmid

   #52581

   Purpose

   Lentiviral transfer vector driving expression of eGFP in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. UbG76V-eGFP-V5His_20aa

    Plasmid

    #23970

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_20aa
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Promoter

    Available since

    March 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Ub[9]-mRFP[1]

    Plasmid

    #20249

    Depositor

    Nico Dantuma

    Article

    Salomons et al Mol Cell Biol. 2009 Jan 21. ():.

    Insert

    ubiquitin C (UBC Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    changed Stop codon 686 to Glycine

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-EPN-01(ΔL1)

    Plasmid

    #80049

    Purpose

    Expression of EPN-01 mutated in TSG101 binding site.

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01(deltaPTAP)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PTAP10AAAA

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-EPN-01*(ΔM)

    Plasmid

    #80040

    Purpose

    Expression or G2A mutant of EPN-01*

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01*(G2A)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Gly2 mutated to Ala

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-EPN-01(ΔL2)

    Plasmid

    #80050

    Purpose

    Expression of EPN-01 mutated in ALIX binding site.

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01(deltaYP)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    YP37AA

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-EPN-01*(ΔL1)

    Plasmid

    #80042

    Purpose

    Expression of PTAP L domain mutant EPN-01*

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    pCMV-EPN-01*(ΔL2)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PTAP sequence in p6 mutated to Ala

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-EPN-01*(ΔL2)

    Plasmid

    #80043

    Purpose

    Expression of YPxL L domain mutantd EPN-01*

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01*(L33R)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    YP37 in p6 mutated to AA

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-EPN-01*-(ΔLF)

    Plasmid

    #80045

    Purpose

    Expression of Vpr binding mutant EPN-01*

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01*(L33R)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Vpr binding site LF45 in p6 mutated

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-EPN-01(ΔL1+L2)

    Plasmid

    #80051

    Purpose

    Expression of EPN-01 mutated in both L domains

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01(deltaPTAP/deltaYP)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PTAP10AAAA, YP37AA

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-EPN-01*(ΔI)

    Plasmid

    #80041

    Purpose

    Expression of EPN-01 assembly mutant L33R

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-01*(L33R)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    changed Leu33 in I3-01 to Ala

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. U6-shRNA pgkCre

    Plasmid

    #24971

    Depositor

    Tyler Jacks

    Article

    Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

    Insert

    shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge