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pAdCMV/V5-DEST-STAT3C-3xFlag

Plasmid

#99264

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

A662C N664C

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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GLUT9-eGFP/pcDNA-DEST47

Plasmid

#18730

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pFRT/FLAG/HA-DEST LIN28A

Plasmid

#43797

Depositor

Thomas Tuschl

Article

Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

Insert

LIN28A (LIN28A Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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221 pCS 3MT DEST

Plasmid

#13070

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

6x Myc Tag

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD-mCherry

Plasmid

#140306

Purpose

Expresses HA-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X FL

Expression

Mammalian

Mutation

contains only the SH4 and UD regions fused to mCh…

Promoter

CMV

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-N (TRP1)

Plasmid

#177797

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST LIN28B

Plasmid

#43798

Depositor

Thomas Tuschl

Article

Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

Insert

LIN28B (LIN28B Human)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

Plasmid

#172535

Purpose

Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

-3.5ubb:plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F and R415H mutations obse…

Promoter

zebrafish ubb

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRW004-SM-destination-proUBQ10-Cas9

Plasmid

#104438

Purpose

Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Plant

Promoter

UBQ10

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD-S32

Plasmid

#140303

Purpose

Expresses Flag-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

lacks the KD region

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE- TRE-DEST-NBioTAP

Plasmid

#53568

Purpose

Lentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIB CMV/TO DEST (w320-1)

Plasmid

#17400

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C1

Plasmid

#19887

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 1 (AGO1 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:mCherry-PA-Rac1

Plasmid

#169044

Purpose

Destination transgenesis vector for expressing mCherry fused with an optimized photoactivatable Rac1 protein in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

PA-Rac-1 (RAC1 Avena sativa (oat), Human)

Tags

6X His and Fused with mCherry

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

3xMnx1

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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370 pTol2-lmo2:CherryDest

Plasmid

#73489

Purpose

Tol2 vector with lmo2 promoter upstream of Cherry, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

lmo2 promoter:mCherry-attR1-attR2

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UBA/UIM-mut

Plasmid

#187152

Purpose

mammalian cell expression of FLAG-HUWE1 M1328A/F1330A/A1376G/S1382A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

M1328A/F1330A/A1376G/S1382A

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-S32-KD

Plasmid

#140299

Purpose

Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

lacks the UD region

Promoter

CMV

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S32-KD

Plasmid

#140311

Purpose

Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Contains only the SH3, SH2, and KD regions

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C4

Plasmid

#19890

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 4 (AGO4 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT-TO-GFP-PALB2 KR

Plasmid

#71107

Purpose

mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

GFP

Expression

Mammalian

Mutation

first 8 Lysine residues mutated to Arginine (K14-…

Promoter

CMV/TO

Available Since

Dec. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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