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pcDNA 3.2/V5-DEST SNAP29-V5

Plasmid

#69821

Purpose

Expresses SNAP29-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Synaptosomal-associated protein 29 (SNAP29 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Promoter

cmv

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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IZ501: pMVP/Lenti-DEST

Plasmid

#121848

Purpose

pMVP destination vector, empty Lentivirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C3

Plasmid

#19889

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 3 (AGO3 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo DEST (w18-1)

Plasmid

#17390

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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LC301: pMVP/PB/Zeo-DEST

Plasmid

#121877

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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GLUT9-eGFP/pcDNA-DEST47

Plasmid

#18730

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pDEST40-2XHA-Src-Y419F

Plasmid

#140316

Purpose

Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Y419F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3C-3xFlag

Plasmid

#99264

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

A662C N664C

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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221 pCS 3MT DEST

Plasmid

#13070

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

6x Myc Tag

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST LIN28A

Plasmid

#43797

Depositor

Thomas Tuschl

Article

Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

Insert

LIN28A (LIN28A Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCWX-R4DESTR2-PGK-BSD

Plasmid

#114320

Purpose

Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Blasticidin resistance

Depositor

Patrick Salmon

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRW004-SM-destination-proUBQ10-Cas9

Plasmid

#104438

Purpose

Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Plant

Promoter

UBQ10

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST LIN28B

Plasmid

#43798

Depositor

Thomas Tuschl

Article

Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

Insert

LIN28B (LIN28B Human)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

Plasmid

#172535

Purpose

Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

-3.5ubb:plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F and R415H mutations obse…

Promoter

zebrafish ubb

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD-mCherry

Plasmid

#140306

Purpose

Expresses HA-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X FL

Expression

Mammalian

Mutation

contains only the SH4 and UD regions fused to mCh…

Promoter

CMV

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE- TRE-DEST-NBioTAP

Plasmid

#53568

Purpose

Lentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIB CMV/TO DEST (w320-1)

Plasmid

#17400

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C1

Plasmid

#19887

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 1 (AGO1 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD-S32

Plasmid

#140303

Purpose

Expresses Flag-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

lacks the KD region

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:mCherry-PA-Rac1

Plasmid

#169044

Purpose

Destination transgenesis vector for expressing mCherry fused with an optimized photoactivatable Rac1 protein in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

PA-Rac-1 (RAC1 Avena sativa (oat), Human)

Tags

6X His and Fused with mCherry

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

3xMnx1

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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