We narrowed to 4,323 results for: Mcc;

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pSB278 - pL0_pT16H2 (pro + 5U)

Plasmid

#203889

Purpose

T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T16H2 promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1224 - pL0_pT3R (pro + 5U)

Plasmid

#203892

Purpose

T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T3R promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1215 - pL0_pDAT (pro + 5U)

Plasmid

#203895

Purpose

DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

DAT promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI or BsaI cut site for MoClo domes…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB55 - pL0_CrGLK (CDS)

Plasmid

#203903

Purpose

MoClo compatible L0 plasmid containing the CrGLK coding sequence

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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3020_pETcon-SARS-CoV-2-RBD_N501Y

Plasmid

#184405

Purpose

yeast surface display of the SARS-CoV-2 Alpha variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV(WT)-mouse-full-length-CHMP3-HA

Plasmid

#154175

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-CHMP3(147)-HA

Plasmid

#154177

Purpose

expresses mouse CHMP3(147) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 147

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZmat3.52.6/Cre

Plasmid

#167854

Purpose

Expresses Cre recombinase and an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSMT3-FBF2 SS/Y

Plasmid

#166973

Purpose

Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575…

Promoter

T7

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSMT3-FBF2 AS/Y

Plasmid

#166974

Purpose

Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575…

Promoter

T7

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ282 (TsCas12a)

Plasmid

#138114

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized TsCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

TsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ283 (MlCas12a)

Plasmid

#138115

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MlCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

MlCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ287 (BsCas12a)

Plasmid

#138123

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1-ORF24-2xStrep

Plasmid

#138454

Purpose

Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1-ORF24 (BcRF1, ORF24 EBV/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

BcRF1 amino acids 1-178 fused to ORF24 amino acid…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-UL87-ORF24-2xStrep

Plasmid

#138455

Purpose

Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87-ORF24 (UL87, ORF24 HCMV/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

UL87 amino acids 1-248 fused to ORF24 amino acids…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ-His6-K2P4.1B-mCherry-StreptagII

Plasmid

#158744

Purpose

Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

Depositor

Arthur Laganowsky

Article

Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

Insert

K2P4.1-B (KCNK4 Human)

Tags

His6 and mCherry with Streptag II

Expression

Yeast

Mutation

Residues 1-290 and N-linked glycosylation sites r…

Promoter

AOX1

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-AGO1 ts2

Plasmid

#115851

Purpose

AGO1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO1 shRNA (AGO1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-AGO2 ts3

Plasmid

#115855

Purpose

AGO2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO2 shRNA (AGO2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMDC32-FIT2-N[80]A

Plasmid

#96992

Purpose

Express mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Expression

Plant

Mutation

Amino acid residue 80 was mutated (N[80]A). Mutat…

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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GPR152-Tango

Plasmid

#66328

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR152 (GPR152 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only