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We narrowed to 11,979 results for: alp

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Showing: 1981 - 2000 of 11979 results

  2. pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

    Plasmid
    #141371
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Article
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    C145A, human codon optimized
    Promoter
    EF1alpha
    Available Since
    April 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141371-plasmid-map-sequence-id-288079

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  5. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid
    #229767
    Purpose
    Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Article
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Insert
    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    Use
    Luciferase
    Tags
    SmBiT
    Mutation
    See Depositor Comments
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229767-plasmid-map-sequence-id-457423

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  8. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

    Plasmid
    #196879
    Purpose
    Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196879-plasmid-map-sequence-id-408686

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  9. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196894
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    N-gTBD-mOrange2 (NEDD1 Human)
    Use
    Cre/Lox; Dre/rox
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196894-plasmid-map-sequence-id-392229

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  10. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid
    #196881
    Purpose
    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-Cdk5rap2-CTD
    Use
    Cre/Lox; Dre/rox
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196881-plasmid-map-sequence-id-391885

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  14. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

    Plasmid
    #47457
    Purpose
    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47457-plasmid-map-sequence-id-69629

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  15. beta catenin binding mutant of alpha catenin sensor

    Plasmid
    #71711
    Purpose
    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin Δβ-cat mutant FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 48- 163
    Promoter
    CMV
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71711-plasmid-map-sequence-id-142367

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  17. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47455
    Purpose
    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    Use
    AAV
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47455-plasmid-map-sequence-id-69627

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  18. Anti-Slo1/BKAlpha maxi-K+ channel (Mouse) [L6/23R]

    Plasmid
    #128634
    Purpose
    Mammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/23.
    Depositor
    James Trimmer
    Article
    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    anti-Slo1/BKAlpha maxi-K+ channel (Mouse) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    128634-plasmid-map-sequence-id-258337

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Showing: 1981 - 2000 of 11979 results